Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"REV1"”.
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The mutation spectra of 4-nitroquinoline N-oxide-induced frameshift reversion ofhis4-38 in REV1 and rev1-1 strains of Saccharomyces cerevisiae
… in Saccharomyces cerevisiae strains XY729 (REV1) and XY760 (rev1-1). These two strains differ in their ability to repair DNA. The REV1 gene is involved in error-prone repair of DNA. The two strains differed in their sensitivity to the lethal effects of 4NQO. To control for this difference …
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DNA damage tolerance and mutagenesis : the regulation of S. cerevisiae Rev1
… polymerases. Specifically, the TLS polymerases, Rev1, Pol [zeta], and Pol [eta], perform TLS in Saccharomyces cerevisiae. In addition to their contribution to cellular survival after DNA damage, Rev1 and Pol [zeta] are responsible for the majority of spontaneous and damage-induced mutagenesis. …
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Novel regulatory mechanisms of mutagenic translesion DNA synthesis : characterization of REV1 in Saccharomyces cerevisiae
Cells are constantly subjected to DNA damage from endogenous and exogenous sources. Spontaneous DNA damage alone accounts for -30,000 DNA lesions per day in a mammalian cell. Cells are also exposed to an enormous variety of environmental agents that can cause a wide range of modified bases and …
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The mutation spectra of frameshift reversion ofhis4-38 in REV1 andrev1-1 strains of Saccharomyces cerevisiae
… in Saccharomyces cerevisiae strains XY729 (REV1) and XY760 (rev1-1). To accomplish this, two methods were employed, the double-strand repair of gapped plasmids, and the polymerase chain reaction (PCR). Both approaches permitted the recovery of DNA sequence alterations from an unmodified …
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Genetic interactions of repriming and translesion synthesis
… interesting group of hits were RAD18, UBE2A and REV1 - three genes with key roles in DNA damage tolerance through translesion synthesis which appeared as hits depleted in PRIMPOL-/- cells in screens undertaken with cisplatin treatment. To further probe this interaction, REV1-/- PRIMPOL-/- cells …
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Structure-Based Drug Design in Medicinal Chemistry I. Development of Translesion Synthesis Inhibitors II. Synthesis and Biological Evaluation of Sulfonyl Piperazine Derivatives for LpxH Inhibition
… promoting mutagenic DNA synthesis. Since Rev1/Pol ζ-mediated translesion synthesis plays an important role in cisplatin-induced mutations, Rev1/Pol ζ interface is an attracted target for inhibition of TLS by small molecules. An ELISA assay has identified two promising hits for inhibition …
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Structure-Guided Design of Novel Therapeutics Targeting Translesion DNA Synthesis and Lipid A Biosynthesis
… called translesion DNA synthesis (TLS). The Rev1 polymerase orchestrates this pathway by recruiting one of three inserter polymerases and the extender polymerase (Pol ζ) to bypass the lesion. Here we report the discovery and characterization of an inhibitor of the protein-protein interaction …
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Protein and Ligand Dynamics in Drug Development and Resistance
… on this project demonstrated that inhibition of Rev1, an important scaffold in the translesion synthesis process, by the compound JH-RE-06 sensitizes cancer cells to cisplatin chemotherapy and prevents drug resistance. Surprisingly, we found that this inhibition occurs through inhibitor-induced …
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!Development of small molecule therapeutics against anti-infectious and anti-cancer drug resistance via structure-based drug design
… and promotes the formation of mutagenic DNA. Rev1/Pol ζ–mediated TLS plays an important role in cisplatin-induced mutations, and thus, the Rev1/Pol ζ interface is an attractive target for small-molecule TLS inhibitors. Herein, we aimed to develop TLS inhibitors as potential anticancer agents …
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Translesion synthesis and mutations: On the mutagenic properties of the two DNA lesions, 8-oxo-G and Pt-GG, and the functions of Y-family DNA polymerases and Rev3L on the bypass of each of the DNA lesions in mammalian cells
… DNA polymerases Pol eta, Pol kappa, Pol iota, Rev1 and Rev3L on bypassing each of the lesions 8-oxo-G and Pt-GG. My study takes advantage of the Next Generation Sequencing (NGS) technology to determine mutagenic effects of the DNA lesions in vivo and effects of translesion DNA polymerases on …
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The identification of novel factors required for the development of primordial germ cells
… the translesion synthesis (TLS) factor Rev1 as a key factor involved in PGCLC development. Furthermore, we validated this requirement in vivo and showed that absence of Rev1 causes a numeric reduction in PGCs during the migratory period of PGC development. We also identified additional …
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Regulación de la rutas de tolerancia a daños replicativos dependientes de RAD6, RAD5 y RAD52 durante el ciclo celular en Saccharomyces cerevisiae
… de PCNA mediada por Rad6/Rad18 y las polimerasas Rev1/Pol z) para reparar las lesiones de ssDNA a través de una función no recombinogénica, y en consecuencia son necesarias para la mutagénesis inducida por daño en el ADN. Específicamente, se requieren Rad52, Rad51 y Rad57, pero no Rad54, para la …
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Regulation of DNA damage tolerance : studies of the translesion synthesis DNA ploymerase eta in Saccharomyces cerevisiae
… DNA. Another eukaryotic TLS polymerase is Rev1, which is thought to play a structural role in TLS by binding the other TLS polymerases. In mammals, Rev and pol eta robustly interact. Here, I show that any physical interaction between Rev and pol eta in S. cerevisiae is too weak or …
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The effect of replication impediments on differentiation
… specific, mechanism. This also occurred in REV1-/- cells, a specialised polymerase known to play a role in G-quadruplex-processing. This work has led to the conclusion that replication impediments are able to alter the course of definitive endoderm differentiation in human induced …
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Molekularbiologische und bioinformatische Analyse des Proteins KIAA0592/RISP und des dazugehörigen Gens
… auf eine C-terminal deletierte Rev-Variante (Rev1-54) übertragen. Trotzdem konnte durch diese Substitution die Funktionalität von Rev nicht wiederhergestellt werden. Eine Crm1-Rekrutierung an Rev ist offensichtlich nicht alleine ausreichend, ein funktionales RRE-mRNA exportierendes Rev-Protein …
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DNA Translesion Synthesis Factors are Essential for Mammalian Embryonic Germ Cell Development
… of TLS initiation (Pcna<sup>K164R/K164R</sup> or Rev1<sup>-/-</sup>) or extension (Rev7<sup>-/-</sup>) there is a >150-fold reduction in PGC number. Consistent with a role for TLS, PGCs in mutant embryos accumulate unresolved DNA damage and have abnormal cell cycle kinetics which are found to be …