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Showing 1 to 7 of 7 for “"REPSA"”.
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REPSA Directed Assessment of Native Cleavage Resistance of DNA to Type IIS Restriction Endonucleases and Modification of REPSA for High Temperature Application
… Endonuclease Protection and Selection Assay (REPSA) to work in high temperature conditions for the discovery of new DNA-binding proteins in thermophiles (HT-REPSA). We utilized <em>Thermus thermophilus </em>(HB-8/ATCC 27634/DSM 579) as a test organism due to its amenable nature in a laboratory …
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Analysis of FokI Cleavage Resistance Observed in DNA Sequences Generated via the Combinatorial Selection Method, REPSA
… including the combinatorial selection method, REPSA. FokI employment in the REPSA procedure has demonstrated the tendency to select for an unmodified sequence that possesses the recognition site yet is refractory to cleavage by the enzyme. Sequencing of the cleavage resistant DNA has revealed …
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Explorations of Thermus thermophilus HB8 Transcription Factors TTHA1437 and TTHA1719 using the Combinatorial Selection Method REPSA
… Protection, Selection, and Amplification (REPSA) has been successful for identifying and validating consensus binding sequences in <em>T. thermophilus </em>HB8 with previously studied TFs. Here REPSA was explored as a technique and method for identifying TTHA1437 and TTHA1719 TFs in <em>T. …
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Intrinsic Cleavage Inhibition by Thermophilic Type II-S Restriction Endonucleases
… Protection, Selection, and Amplification (REPSA). However, laboratories generated evidence that intrinsically cleavage refractory sequences exist. Understanding the pervasiveness of these sequences and their potential effects on the utilization of IISREs can be observed and analyzed through …
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Specificity of triple helix formation
… Protection, Selection and Amplification’ or REPSA. REPSA has<br/>been used to select for DNA templates that are bound by the 9mer TFO containing<br/>six bis-amino-U residues. Fourteen of the sequences which emerged from<br/>REPSA were chosen for footprinting with TFOs containing BAU, …
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Discovery of a DNA-binding Consensus and Potential Genomic Regulatory Binding Sites for the Thermus thermophilus HB8 Transcriptional Regulator TTHA1359
… Protection, Selection, and Amplification (REPSA) has been applied to study TTHA1359, one of the four CRP/FNR superfamily TFs in the model organism <em>T. thermophilus</em> HB8. A TTHA1359-binding consensus, 5’-A(T/A)TGT(G/A)A(N<sub>6</sub>)T(C/T)ACA(A/T)T-3’, was identified using REPSA to …
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Determination and Dissection of DNA-binding Specificity for the Thermus thermophilus HB8 Transcriptional Regulator TTHB099
… Protection, Selection and Amplification (REPSA), we identified the preferred DNA-binding motif for TTHB099, 5'-TGT(A/g)n(t/c)c(t/c)(a/g)g(a/g)n(T/c)ACA-3', and mapped potential binding sites, and regulated genes within the <em>T. thermophilus</em> HB8 genome. Comparisons with expression …