Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"REP-PCR"”.
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American Alligator Fecal Coliform and Pathogen Identification, Genotypic Fingerprinting, and REP-PCR Bacterial Source Tracking in South Carolina
… The second component evaluated the ability of Repetitive Extragenic Palindromic PCR (REP-PCR) to produce identifiable, unique, and stable genomic fingerprints from alligator fecal coliform and potential pathogenic bacteria. The final component determined if alligator fecal coliform and …
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Caracterização da microbiota lática do Queijo Minas artesanal produzido na região do Serro, Minas Gerais, por métodos cultura dependente e independente
… isolados (n = 176) que foram caracterizados por Repetitive extragenic palindromic-PCR (Rep-PCR) e identificados por sequenciamento da região V1-V3 do 16S rRNA. Em paralelo, as amostras foram submetidas a extração de DNA total para caracterização da diversidade microbiana por Rep-PCR, Denaturing …
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A Genotypic Comparison of Staphylococcus lugdunensis Clinical Isolates of Diverse Geographic Origin
… arbitrarily primed polymerase chain reaction (AP-PCR), inter repetitive sequence polymerase chain reaction (REP-PCR), and southern hybridization.|PFGE together with southern hybridization data proved superior to both types of PCR based methods. PFGE produced distinct banding patterns that were …
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Análise da variabilidade de Magnaporthe grisea no Estado de Santa Catarina
… SC. Os isolados foram analisados através de rep-PCR baseado no transposon Pot-2. Para a identificação das raças, um subgrupo de isolados foi inoculado na série internacional de cultivares diferenciais de arroz. As raças identificadas tiveram seu espectro de virulência avaliado em um grupo de …
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An evaluation of DNA fingerprinting methods for subtyping Salmonella
… (RFLP) analysis, and polymerase chain reaction (PCR) fingerprinting methods were evaluated. Plasmid analysis, including plasmid profiles and plasmid fingerprints have been shown to be useful primarily in short-term investigations of disease outbreak. However, plasmid profiles or possession of …
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Molecular Characteristics and Dissemination Mechanism of Vancomycin-Resistant Enterococci: A Tertiary vs. Non-Tertiary Health Care Hospitals
… (MIC)s of vancomycin and teicoplanin, multiplex PCR for resistance genotype, structural analysis of Tn1546, multilocus sequence typing (MLST), virulence factor (esp), and dendrogram of repetitive extragenic palindromic PCR (rep-PCR) were analyzed in all VREF isolates. All the isolates harboured …
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Enumeration, isolation, identification and probiotic characterisation of lactic acid bacteria from Nigerian human breast milk
… was carried out by grouping the isolates using repetitive sequence based PCR (rep-PCR). A total of nineteen groups were generated from the rep-PCR DNA profiles. This was followed by genotypic identification using 16S rRNA gene sequencing. The 16S rRNA gene sequencing identified LAB and non-LAB. …
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Technological features and ability to produce nisin by Lactococcus lactis subsp. lactis bv. diacetylactis obtained from dairy environment
… and dyacetil production, despite specific PCR assays), fingerprinted by rep-PCR and characterized for their technological potential: lactofermentation patterns, extracellular proteolytic activity, acidification capacity, and resistance to NaCl. Subsequently, the strains were subjected to …
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Molekulargenetische Untersuchungen zur Persistenz und Genomvariabilität von Escherichia-coli-Isolaten von Patientinnen mit chronisch rezidivierenden Harnwegsinfektionen
… untersucht. Weiterhin wurde mit Hilfe der Rep-PCR und der Pulsfeldgelelektrophorese ein Identitäts-Screening durchgeführt, so daß sämtliche E. coli-Isolate zu 46 Stuhl- und 16 Urinklonen zusammengefaßt werden konnten. Alle untersuchten Gene fanden sich bei den Urinklonen häufiger als bei …
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Identification and characterisation of cephalosporins and carbapenem-resistant Klebsiella pneumoniae isolates from Misrata, Libya
… and carbapenems respectively, have been reported from several countries globally and recently among Libyan combatants who have been transferred to European countries for advanced medical care. However, there is a lack of data about their presence in Misrata and in Libya in general. This …
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Probiotic characterization of Bifidobacterium spp. isolated from commercial fermented milk in the UK
… recovery of the bifidobacteria and ease of preparation, it was concluded that TOS-MUP is the medium of choice for determination of total viable counts of bifidobacteria in fermented milks. The result of enumeration study indicated that most of the tested products (22 out of total 24) on …
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Characterization of lactic acid bacteria from Nono, a Nigerian naturally fermented milk product
… system. Genotypic methods including repetitive element sequence-based polymerase chain reaction (rep-PCR) and sequencing of the 16S rRNA, phenylalanyl-tRNA synthase a-subunit (pheS) and RNA polymerase, alpha subunit (rpoA) genes were used to identify the bacteria. The sequences were …
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Developing starter cultures for the optimisation of cassava (Manihot esculenta Crantz) fermentation
… Fermented cassava pieces were examined to detect reported bacteria (Bacillus, Clostridium spp.) associated with softening by conventional cultural and non-cultural methodology; to detect pectinolytic activity by viscometry and dinitrosalicylic acid reagent colorimetric methods; to evaluate …
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Genetic Characterization and Manipulation of Solvent -Producing Clostridia
… are not found within the plasmids pTJ1 and pTJ2. PCR data suggested that these sequences were not present in the parent C. beijerinckii SA2. Results of rep-PCR experiments indicated that C. beijerinckii SA2TJ1, C. beijerinckii SA2TJ1 sol, C. beijerinckii SA2TJ2, and C. beijerinckii SA2TJ2sol are …
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Evolution of Pseudomonas aeruginosa in the Cystic Fibrosis Lung
… patterns of genomic changes in 49 strains representing 16 longitudinal series that ranged up to 25 months in duration. A subset of these contained mutators, operationally defined as producing RifR mutants at a frequency one order of magnitude or greater than the population median …
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Evolution of Pseudomonas aeruginosa in the Cystic Fibrosis Lung
… patterns of genomic changes in 49 strains representing 16 longitudinal series that ranged up to 25 months in duration. A subset of these contained mutators, operationally defined as producing RifR mutants at a frequency one order of magnitude or greater than the population median …
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Characterization of probiotic Lactobacillus spp. isolates from commercial fermented milks
… targeted lactobacilli and also simplicity of preparation, MRS-Clindamycin was chosen as the best medium for enumeration of probiotic Lactobacillus in fermented milks. The results of enumeration of lactobacilli showed that 22 out of a total 36 tested products contained more than 106 colony …