Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 47 for “"RBPs"”.
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Genetic screens of RNA binding proteins identify a role for N6-methyladenosine in promoting B cell differentiation
… is mediated in part by RNA binding proteins (RBPs). Roles for RBPs have emerged in the immune system but remain largely unexplored. My project utilised high-throughput genetic screens to identify roles for RBPs in lymphocytes. Initially, I generated custom sgRNA libraries targeting RBPs to …
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Biochemical and functional characterization of human RNA binding proteins
… approximately one thousand RNA binding proteins (RBPs) that direct RNA splicing, cleavage and polyadenylation, localization, translation, and degradation. Despite the central role of RBPs in RNA processing and gene expression, they have been less well studied than DNA binding proteins, in part due …
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A Census of Human RNA-Binding Proteins and Characterization of the DEDDh RNA Exonuclease NEF-sp in the 3' End Maturation of 28S Ribosomal RNA
… stability and translation. RNA-binding proteins (RBPs) and ribonucleoprotein (RNP) complexes coordinate these processes. RBPs are central to cellular metabolism and their role in human diseases has been widely studied. Recent large-scale quantitative methods such as nextgeneration sequencing and …
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Multiple regulatory layers in the establishment of Rbfox2 splicing networks
… splicing is mediated by RNA binding proteins (RBPs) recognizing short sequence motifs in nascent transcripts. The Rbfox RBPs are highly conserved splicing factors that regulate tissue-specific exon inclusion by binding the RNA motif UGCAUG. We sought to comprehensively define the Rbfox2 …
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The evolution, modifications and interactions of proteins and RNAs
… modifications (PTMs) in RNA-binding proteins (RBPs). By comparing occurrences of PTMs, I observe that RBPs significantly undergo more PTMs than non-RBPs. I also found that within RBPs, PTMs are more frequently targeted at regions that directly interact with RNA compared to regions that do not. …
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Co-regulation of the Smooth Muscle Cell Alternative Splicing Programme by the RNA-Binding Proteins RBPMS, RBFOX2, MBNL1, and QKI
… between RBPMS and other RNAbinding proteins (RBPs) in this regulatory network remain unclear. In this study, I investigated RBFOX2, MBNL1, and QKI as potential co-regulators of RBPMS in VSMC AS regulation. RBFOX2 was identified as a key candidate due to enrichment of its GCAUG binding motifs …
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Investigating the Role of RNA-Binding Proteins in Tumor Response to DNA Damage-Inducing Chemotherapy
… studies indicate that RNA-binding proteins (RBPs) appear to play a particularly important role in cancer, and may contribute to chemoresistance, but the field is still at an early stage and nothing has yet progressed towards translational utility. This is at least partially due to a lack of …
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Biochemical and Functional Characterization of Novel RNA-binding Proteins Interacting with SMN in Motor Neuron-derived Cells
… between SMN and two RNA-binding proteins (RBPs) known to be components of axonal RNA granules. More specifically, we demonstrated that SMN interacts with HuD and SERBP1 in a direct fashion in foci-like structures along neurites of motor neuron-derived cells. We have also demonstrated that …
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RNA binding proteins ZFP36 and ZFP36L1 limit CD8+ T cell differentiation and effector function
… is mediated in part by RNA binding proteins (RBPs). The ZFP36 family of RBPs are key regulators of gene expression in the immune system. The founding member ZFP36 is a well-known regulator of cytokine mRNA stability. Paralogs ZFP36L1 and ZFP36L2 act redundantly during thymopoiesis and in …
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Inferring Transcriptional and Post-Transcriptional Network Structure by Exploiting Natural Sequence Variation
… binding specificity of RNA-binding proteins (RBPs). We first analyzed a recent compendium of in vivo mRNA binding data to model the sequence specificity of 45 yeast RBPs in the form of a position- specific affinity matrix (PSAM). We were able to recover known consensus nucleotide sequences for …
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Mechanisms of Dysregulated Alternative Splicing in Cardiac and Skeletal Muscle under Diabetic Conditions
… and controls RNA-binding proteins (RBPs) CELF1 and RBFOX2 that are involved in developmentally regulated AS in the heart. Since PKC is chronically activated in diabetes, we hypothesized that aberrant PKC activity alters CELF1 and RBFOX2 and disrupts AS. To test this hypothesis, we …
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Etablierung eines quantitativen transienten Expressionssystems zur funktionellen Charakterisierung von RNA-Bindeproteinen in der posttranskriptionellen Genregulation
RNA-Bindeproteine (RBPs) regulieren eine Vielzahl von zellulären Anpassungsprozessen. Da sie stark durch exogene Faktoren beeinflusst werden, binden sie häufig unterschiedliche Zieltranskripte. Zur Identifizierung der Zieltranskripte in vivo wurde individual nucleotide crosslinking and …
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Computational analysis of cooperation and competition mechanisms between RNA-binding proteins
… forming this network, RNA-binding proteins (RBPs) play key roles in post-transcriptional regulation of gene expression (PTR). Understanding RBPs behavior is therefore crucial given their participation in all stages of the mRNA life cycle. Although an increasing amount of work has highlighted …
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Principles of HuR-RNA targeting, interaction dynamics, and functional outcomes
… logic of gene expression. RNA-binding proteins (RBPs) and non-coding RNAs are the regulatory molecules primary responsible for interaction with target mRNAs and thereby regulating post-transcriptional processes eventually influencing characteristics of the encoded protein. Many of the mRNA …
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Functional characterization of the RNA binding protein RALY
… high variety of cells. The RNAbinding proteins (RBPs) play crucial roles in this variability by regulating many steps of biological processes regarding RNA metabolism. The heterogeneous nuclear ribonucleoproteins (hnRNPs) belong to big family of RBPs involved in many aspects of RNA metabolism …
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CELF1, PTBP1, and RBFOX2-mediated alternative splicing regulation in cardiovascular diseases
… that dysregulation of the RNA binding proteins (RBPs) CELF1, PTBP1, and RBFOX2 contribute to AS changes. Utilizing genome-wide approaches, we identified extensive changes in AS patterns in T1D mouse hearts. We discovered that many aberrantly spliced genes in T1D hearts have CELF1 and PTBP1 …
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Exploiting network-based approaches for understanding gene regulation and function
… regulatory networks formed by RBPs. I discuss the sequence attributes and functional processes associated with RBPs, methods used for the construction of the networks formed by them and finally examine the structure and dynamics of these networks based on recent publicly …
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Integrative analysis of transcriptomic data to elucidate regulators of pre-mRNA processing
… resource for investigating APA regulation by RBPs. Finally, Chapter 6 introduces the Comparative Analysis of Alternative RNA Processing (CAARP), a lightweight framework and repository of reproducible analysis scripts in the form of iPython notebooks that support flexible and extensible …
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Precise Temporal Regulation of Ribonucleoprotein Complexes during the Drosophila Maternal-to-Zygotic Transition
… transition (MZT). RNA-binding proteins (RBPs) tightly regulate the translation, stability, and localization of maternal mRNAs through post-transcriptional regulatory mechanisms. For example, in the Drosophila melanogaster embryo, the Smaug (SMG) RBP forms ribonucleoprotein complexes with …
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Uncovering the Roles of Human C2H2-Zinc Finger Protein ZNF121 in Gene Regulation
… for its mRNA targets. RNA-binding proteins (RBPs) bind transcripts to exert regulatory effects, and many DNA-binding C2H2-zinc finger proteins (ZFPs) have recently been shown to be RBPs with roles in mRNA biology. I identified C2H2-ZFP, ZNF121, as a YTHDF2 binding partner in affinity …
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