Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"RBL-2H3"”.
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Mechanical Deformation of the Extracellular Matrix Mediates Cellular Activation and Mechanotransduction
… loading (5% and/or 10% peak strain) had on 1) RBL-2H3 cell degranulation; 2) the influences RBL-2H3 cells have on fibroblast function; and 3) age-dependent differences in cardiac fibroblast gene expression. In addition, we examined the differences in ECM remodeling behavior by neonatal and …
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Determining the Roles of Q-SNAREs in Mast Cell Tumor Necrosis Factor Exocytosis
… used Stx2 KO BMMCs as well as Stx3 and Stx11 KD RBL-2H3 cells in secretion assays to determine the consequences of depleting these isoforms on TNF secretion. The resulting data indicates that while Stx2 KO does not impact either TNF secretory pathway, Stx3 KD partially inhibited pre-formed TNF …
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Vitamin E und der vesikuläre Transport : Untersuchungen zu den genregulatorischen Funktionen von Vitamin E mittels Microarray- und real time PCR-Analysen in der Maus und funktionellen in vitro Assays in RBL-2H3 Zellen
… Assays in der sekretorischen Mastzelllinie RBL-2H3 gezeigt werden. Die Inkubation der Zellen mit α-Tocopherol resultierte in einer konzentrationsabhängigen Erhöhung der PMA/Ionomycin-stimulierten Sekretion der β-Hexosaminidase. Eine erhöhte Expression ausgewählter Gene, die an der …
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Anti-oxidant Action and Anti-aging Activity of Phenylpropanoid Compounds
… acid release and prostaglandin E₂ assay in RBL 2H3 mast cells were measured and NIH 3T3 cell was used for collagen synthesis assay. Balb-c mouse were used to measure collagen assay, MPO assay, PLA₂ activity and lipid peroxidation in vivo system. Result: In DPPH radical scavenging activity, …
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Herstellung und Charakterisierung monoklonaler Antikörper gegen die Anaphylatoxin-Rezeptoren
… Antikörper gegen den humanen C5aR wurden RBL-2H3-Transfektanten eingesetzt, die den Rezeptor auf der Oberfläche exprimieren. Nach Bestimmung der Rezeptorspezifität wurden acht monoklonale Antikörper identifiziert, die den C5aR immunhistochemisch erkannten. Anhand ihrer Reaktivität gegen …
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Polymorphisms of High-affinity IgE Receptor Genes in Patients with Aspirin-Intolerant Chronic Urticaria
… activity than that with the -344C allele in RBL-2H3 cell line (p < 0.001). Transcription factor Myc-associated zinc finger protein (MAZ) was bound to the FcεRIα -344C allele probe (-354/-334) with the higher affinity than that of the -344T allele probe. There were no significant differences …
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Effect of phosphorylcholine-based small molecule analogues of the immunomodulatory nematode product ES-62 on mast cell function
… these molecules prevented degranulation in RBL-2H3 cells which is a rat mast cell line. In addition, these molecules reduced levels of IL-6 and TNF-α produced by BMMC activated by cross-linking bound IgE or ionomycin/PMA. Finally, immunofluorescent staining showed that SMAs 63 and 64 and in …
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Unraveling the Molecular Mechanisms of Munc18 and Munc13 in Mast Cell Exocytosis
… thoroughly. Using Rat Basophilic Leukemia-2H3 (RBL-2H3) mast cells, the structure and interactive properties of Munc18 and Munc13 in respect to mast cell exocytosis were examined. Through knockdown and rescue approaches, results indicate that Munc18 protein is crucial for exocytosis of mast …
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Airway Inflammatory States in Response to Environmental Pollutants and the Influence of Oxidative Balance
Asthma continues to grow as a major health issue worldwide. Epidemiologic evidence points to a link between urban air pollution and exacerbation of asthma cases. The pathophysiology of airway inflammation and hyperresponsiveness in relation to air pollution is not well understood. Based upon …