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Showing 1 to 20 of 28 for “"RB51"”.
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Expression of Bacillus Anthracis Protective Antigen in Vaccine Strain Brucella Abortus Rb51
… against brucellosis. B. abortus strain RB51 has been shown to be useful in eliciting protective cell mediated immunity and humoral responses against Brucella in cattle and other animal species. Since the protective antigen (PA) of B. anthracis is known to induce protective antibodies, it …
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Brucella abortus RB51 vaccine: Testing its Spectrum of Protective and Curative Characteristics
… official brucellosis vaccine in the US is strain RB51 (RB51). It elicits protective cell-mediated immunity (CMI) against BA infections. Mycobacterium avium subspecies paratuberculosis (MAP) causes paratuberculosis in ruminants. It is a slow growing intracellular parasite that requires CMI for its …
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Expression and Localization of Green Fluorescent Protein in B. abortus strain RB51
… and undulant fever in human. B. abortus strain RB51 (Strain RB51) is the official vaccine for bovine brucellosis in the USA. B. abortus strain RB51 can be used as a vector for the over-expression of its own (homologous) as well as heterologous protective antigens. The immune system can detect …
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NOVEL STRATEGIES TO DEVELOP BETTER BRUCELLOSIS VACCINES USING BRUCELLA ABORTUS RB51 AND B. NEOTOMAE
… species in some hosts. B. abortus strain RB51 is a stable rough attenuated mutant which is used as a licensed live vaccine for bovine brucellosis in the United States and several other countries. Previous studies have shown that the wboA gene, which encodes a glycosyltransferase required …
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The behavior and effects of Brucella abortus rough strain RB51 in mice and cattle
Brucella abortus st. RB51 is a rough mutant of smooth st. 2308 devoid of O-side chain and resistant to rifampin. The purpose of this investigation was to study the behavior and effects of viable st. RB51 organisms in inoculated mice and cattle and to further substantiate the lack of O-side chain …
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Development of Brucella abortus RB51 as a Vaccine to Protect Against Brucellosis and Anthrax
… against brucellosis. B. abortus strain RB51 has been shown to be useful in eliciting protective CMI and antibody responses against Brucella in cattle and other animal species. Since the protective antigen (PA) of B. anthracis is known to induce antibodies, the pag gene encoding PA was …
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Development and testing of recombinant B. abortus RB51 vaccine strains carrying M. tuberculosis protective antigens
… the efficacy of the B. abortus vaccine strain RB51 as a platform for expression of M. tuberculosis antigens (Ag85B, ESAT6 and Rv2660c) and induction of a protective immune response against M. tuberculosis and B. abortus challenge in mice. Here we report the construction of two recombinant …
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Immune mechanisms in murine brucellosis: studies with strain RB51, a rough mutant of Brucella abortus
… smooth strain 2308. B. abortus rough strain RB51 does not induce an o-side chain specific antibody response and yet protects mice against smooth strain 2308. Passive transfer experiments using serum and nylon wool enriched T cells obtained from micevaccinated with strain 19 and strain RB51 …
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The Humoral Immune Response of Elks (Cervus elaphus nelsoni) and Mice to Vaccination with Brucella abortus Strain RB51
Vaccine Brucella abortus strain RB51, unlike the wild strain 2308 and another vaccine strain (strain 19) does not induce anti-O-chain antibodies. An efficacious vaccine strain that fails to produce an O-chain and thus a lack of an anti-O-chain humoral response greatly simplifies identification of …
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Brucella abortus Strain RB51 Outer Membrane Vesicles as a Vaccine Against Brucellosis in a Murine Model
… cattle and causes brucellosis. B. abortus strain RB51 is a live, attenuated vaccine licensed for cattle. However, there is no available vaccine to prevent human brucellosis. Outer membrane vesicles have been tested as potential vaccines to prevent diseases caused by bacterial species. OMV are …
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Approaches towards vaccine development against Neospora caninum
… and SRS2 were expressed in B. abortus strain RB51 to create recombinant vaccine strains. C57BL/6 mice were vaccinated with either the recombinant strains or the irradiated tachyzoites. Antigen specific IgG2a and IgG1 responses and high levels of interferon gamma and IL-10 were induced by …
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The ability of TLR agonists to upregulate Brucella abortus strain RB51 mediated protection in a murine respiratory model
… vaccine for humans. Brucella abortus strain RB51, approved for use in cattle, provides protection by initiating a strong T-helper 1 (Th1) type response is a candidate vaccine. Based on a model for aerosol exposure mice were vaccinated intranasally (IN) with strain RB51 and challenged IN with …
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Induction of Protection, Antibodies and Cell Mediated Immune Responses by Brucella Abortus Strain RB51, Ochrobactrum Anthropi and Recombinants Thereof
… vaccines. In this research, B. abortus strain RB51 and a closely-related, nonpathogenic Ochrobactrum anthropi (strain 49237) bacterium were used to study the immune response against brucellosis in mice. Both O. anthropi strain 49237 and recombinant strain 49237 expressing Brucella protective …
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The pregnant mouse model of brucellosis: the pathology and protection studies comparing Brucella abortus strains 2308, 19 and RB51
… one virulent (2308) and two attenuated (19 and RB51) strains of B. abortus in the pregnant mouse. When 10<sup>5.7</sup> strain 2308 organisms were administered intraperitoneally to BALB/c mice in midgestation, by 9 days post-inoculation they consistently produced a severe, necro-suppurative …
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Unraveling the host innate immune response to a respiratory model of Brucella abortus
… Protection by B. abortus rough vaccine strains RB51 and RB51SOD is through strong CD4⁺ Th₁ and CD8⁺ Tc₁ adaptive immunity. However, limited information is available on how they stimulate innate immunity. This knowledge is critical for improving these vaccines for their potential use in humans. …
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Mathematical models of immune responses following vaccination with application to Brucella infection
… Brucella abortus vaccine strain 19 and strain RB51. Both vaccines have been shown to be effective, but the mechanisms of immune responses following vaccination with either of the vaccines are not understood yet. In this thesis we analyze the immunological data obtained through vaccination with …
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Intracellular growth of Brucella abortus and B. melitensis in murine macrophage-like cell lines and partial characterization of a biologically active extract from B. abortus strain RB51
… mutant. Vaccination with live B. abortus strain RB51 protects both mice and cattle against challenge with virulent strain 2308. As B. abortus is a facultative intracellular pathogen, the host's cell-mediated immunity is assumed to be important in the clearance of the bacteria. Therefore, selected …
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Rough leucine auxotrophic strains of <i>Brucella</i> expressing <i>Salmonella</i> flagellin C conjugated gonadotropins, an immunocontraceptive brucellosis vaccine for feral swine population control
… a proven stable strain of <i>B. abortus</i> RB51 (a USDA approved cattle vaccine) was used to investigate its effects on fertility in mice when expressing fliC-Gonadotropins. Strains <i>B. abortus</i> RB51 ΔleuB pNS4-trcD-fliC-porcineFSHβ (RB51LFSHβ) and <i>B. abortus</i> RB51 ΔleuB …
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Genetic and Immunological Analyses of a Brucella abortus Protein Exhibiting Lectin-like Properties
… of this protein in B. abortus vaccine strain RB51 was achieved using Brucella groE and sodC promoters as well as its own promoter. Protection and clearance studies were performed in mice to determine the role of this protein in Brucella immunity and pathogenesis. Inoculation with either strain …
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Evaluation of the Aging Immune System Using a Mouse Model of Brucella Infection
… also vaccinated with vaccine B. abortus strain RB51 over-expressing Cu-Zn superoxide dismutase (SOD) and then challenged with B. abortus strain 2308 to determine the effect of vaccination in old vs. young mice. Specific IgG1 and IgG2a response to Brucella antigens were also evaluated to …
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