Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"RAW 264.7 cells"”.
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Phytochemical and pharmacological studies on some endemic Yucatecan medicinal plants
… The initial cytotoxic evaluation against HeLa cells at two fixed concentrations (100 and 33.3 µg/mL) identified the root extracts f J. flammea, S. foetidissimum subsp. gaumeri and S. yucatanensis, and the stem/bank extract of S. adiantoides as the most active extracts. The crude extract of …
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The significance of cell-surface α2,3-linked Sialic Acid in Osteoclasts
<p>Osteoclasts are giant, multinucleated cells that, alongside osteoblasts, are central to maintaining physiologically healthy bone. The functions of osteoclasts and osteoblasts-degrading and depositing bone matrix, respectively-are paired in healthy bone tissue, thereby yielding no net bone loss …
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Phenotypic characterisation of wild type and mutant strains of adherent invasive Escherichia coli and Shigella, in vitro, ex vivo and in vivo models
… Murine cell line cultures in vitro (the RAW 264.7 macrophage cell line) and ex vivo (intestinal organoids derived from), were used together with in vivo (Galleria moth larvae) models to evaluate and ascertain the role of these genes in the pathogenesis of AIEC and Shigella.;The …
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Effect of Scutellaria Baicalensis on Reduction of Radiation/chemotherapy induced Inflammation
… changes, respectively. For in vitro study, RAW 264.7 cells were untreated or treated with S for 30min before exposure to 100ng/ml lipopolysaccharide(LPS). At the indicated time points following LPS stimulation, cells were harvested. To examine effect of S on transcription of the various …
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Multi-omic Approaches to Investigate the Inflammatory Response in Activated Macrophage Cells
… of cultured murine macrophages (RAW 264.7 cells) subjected to<br/>inflammatory stimuli. Cells were treated with immune modulators that either<br/>modelled bacterial infection (Kdo2-Lipid A) or reflected the signals produced by<br/>damaged or dying cells during clearance (ATP). …
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The role of AcrA in the antibiotic resistance and virulence of Salmonella enterica serovar Typhimurium
… ability of S. Typhimurium to infect INT-407 and RAW 264.7 cells \(in vitro\) as previously published for AcrB and TolC mutants. The virulence defect of the mutants could not be rescued by addition of supernatant from an infection of INT-407 cells with SL1344 or addition of media conditioned by …
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Propriedades anti-inflamatórias e antioxidantes dos extratos aquosos das folhas de Turnera subulata Sm. E Anacardium occidentale L.
… model of using lipopolysaccharide macrophage RAW 264.7 cells. Therefore, we quantified the inflammatory response markers in the lineage, as well as its ability to modulate the MAPKs (p38, ERK½ and JNK). In addition, MTT and SRB viability tests, TNF-α and IL-1β modulation evaluation were …
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Differenziamento macrofagico: gli effetti dei polimeri sintetici
… and the effect of their interaction with cells, in particular with the cell populations involved in the inflammatory responses, i.e. monocyte/macrophages. In consideration of what above said, the aim of this study is the comprehension of the in vitro effect of PAI, PVA and PU on three cell …
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Development and Evaluation Of HIV gp120 Responsive Microbicide Formulation for the Prevention of HIV Sexual Transmission
… keratinocytes (VK2/E6E7) and murine macrophage [RAW 264.7 (TIB 71)] cell lines. 𝐶+𝑀𝑅𝑃 average size and ζ-potential were 1130±15.72 nm [PDI = 0.153] and 15.1±0.55 mV, (n=3). Similarly, 𝐶−𝑀𝑅𝑃 average size and ζ-potential were 1089±23.33 nm (n=3) and -14.2±0.25 mV (n=3). Tenofovir (TFV) …
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Evaluation of the antioxidant activity of extracts and flavonol glycosides isolated from the seed coats of coloured beans (Phaseolus vulgaris L.)
… crude extracts in LPS-induced murine macrophage RAW 264.7 cells showed both anti- and pro-inflammatory effects. Extracts of Windbreaker and Eclipse decreased TNF-α levels, suggesting anti-inflammatory properties, while other varieties showed increased levels of TNF-α production, or …
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Untersuchung von Zinksignalen während Aktivierung und Reifung in Monozyten
… tumor necrosis factor-alpha secreting cells, and were essential for LPS-induced signal transduction. LPS-induced zinc signals were repetitive and derived from intracellular stores in Raw 264.7 cells. This was partially mediated by tyrosine kinases, as demonstrated by a screening with 16 …
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Chemokinaktivität induziert durch Interleukin-6
… IL-6 induces directly chemotactic migration of T-cells. In the study presented here the biological properties of IL-6 which are crucial for the migration of monocytes have been analyzed in detail. The first crucial step for leukocyte recruitment is the activation of integrins. Here it is shown …
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Development of versatile drug delivery strategies using PLGA nanoparticles
… strategies to deliver various drugs to targeted cells were examined.<br/><br/>A NP formulation for the intracellular delivery of plasmid DNA was developed, whereby the DNA was encapsulated within PLGA NP by combining salting out and emulsion-evaporation processes. This process reduced the …
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Phagosome proteomes in activated macrophages
… foreign material such as microbes or apoptotic cells to form phagosomes which then fuse with endosome and finally lysosomes, where the particles are finally degraded. My project aims at investigating the phagosome functions regulated in activated macrophages and further exploring the mechanism …
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Fumarate Inhibits TNF-α Release
… TNF-α released from the mouse monocyte cell line RAW 264.7, with no effect on cell viability or reactive oxygen species (ROS) generation. Monomethyl fumarate (MMF) and DMF showed minimal effects on cell viability or ROS generation. We found that DMF at 10µM is anti-inflammatory with minimal …
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Development and Evaluation of 3D-Printed Cardiovascular Stents
… effects on the human umbilical vein endothelial cells (HUVECs). The controlled release of RSL from the stent enhanced nitric oxide (NO) production, lowered the levels tumor necrosis factor (TNF)-α, and alleviated H2O2-induced oxidative stress in HUVECs. The RSL-loaded 3D stents displayed maximum …
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Studies of fluorescent imaging for mRNA detection in living cells
… focuses on the study of imaging mRNA in living cells. To achieve this research objective, three approaches have been utilized:: 1) Imaging of a transgenic mRNA tagged by multiple repeats of malachite green: MG) binding aptamer.: 2) Imaging of inducible nitric oxide synthase: iNOS) mRNA by …
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Turn-on fluorescent probes for detecting nitric oxide in biology
… Cu(II) Complexes that Can Be Trapped in Living Cells: A series of symmetrical, fluorescein-derived ligands appended with two derivatized 2- methyl-8-aminoquinolines were prepared and spectroscopically characterized. The ligands …