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Showing 1 to 20 of 52 for “"RAW 264.7"”.
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In vitro Differenzierung von Monozyten der Zelllinine RAW 264.7 zu Osteoklasten, deren Charakterisierung und Wechselwirkung mit Osteoblasten
… Schlussfolgerungen: 1. Monozyten der Zelllinie RAW 264.7 lassen sich, wie bereits in der Literatur beschrieben, durch Zugabe von M-CSF und RANKL zu osteoklastenähnlichen Zellen differenzieren. 2. Die Osteoklastogenese lässt sich anhand der Veränderung verschiedener osteoklastenspezifischer …
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The in vitro effects of diallyl trisulfide on osteoclast formation and function in RAW 264.7 murine macrophages
… differentiation and function by utilising RAW 264.7 murine macrophages. In this study, various methods were carried out to establish the effects of DATS on osteoclast formation and function in RAW 264.7 murine macrophages. Using a resazurin assay, 1-5 µM of DATS was shown not to affect the …
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Phagosomal escape, sopravvivenza intracellulare e formazione di varianti di piccole colonie di Staphylococcus aureus USA300 nei macrofagi murini RAW 264.7
… sono stati utilizzati macrofagi murini RAW 264.7 infettati con il ceppo di S. aureus ATCC BAA-1556, appartenente al linaege USA300, e trattati con 32 mg/L di eritromicina e/o 20 mM di carnosina. Gli SCV sono stati isolati in tutte le condizioni sperimentali, ma solo quelli ottenuti …
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Phytochemical and pharmacological studies on some endemic Yucatecan medicinal plants
… (IC50 = 28.61 ± 2.27 µg/mL). When tested against RAW 264.7 cells, the water fraction also showed significant activity (IC50 = 10.60 ± 1.83 µg/mL). The water fraction was subjected to chromatographic fractionation using open silica gel columns resulting in the isolation of a saponin as the most …
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The significance of cell-surface α2,3-linked Sialic Acid in Osteoclasts
… however, we were unable to reproduce-in RAW 264.7 cells-the morphological changes previously seen in primary bone marrow macrophages following cellsurface a2,3-linked sialic acid ablation. Due to inherent variation between replicate experiments arising from the use of RAW 264.7 cells, …
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Phenotypic characterisation of wild type and mutant strains of adherent invasive Escherichia coli and Shigella, in vitro, ex vivo and in vivo models
… Murine cell line cultures in vitro (the RAW 264.7 macrophage cell line) and ex vivo (intestinal organoids derived from), were used together with in vivo (Galleria moth larvae) models to evaluate and ascertain the role of these genes in the pathogenesis of AIEC and Shigella.;The …
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Differenziamento macrofagico: gli effetti dei polimeri sintetici
… U937 pro-monocytes, THP-1 monocytes and RAW 264.7 macrophages. Cytotoxicity was evaluated by measuring the rate of viability with MTT, Neutral Red and morphological analysis at light microscope in time-course dependent experiments. The influence of these polymers on monocyte/macrophage …
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Development and Evaluation Of HIV gp120 Responsive Microbicide Formulation for the Prevention of HIV Sexual Transmission
… keratinocytes (VK2/E6E7) and murine macrophage [RAW 264.7 (TIB 71)] cell lines. 𝐶+𝑀𝑅𝑃 average size and ζ-potential were 1130±15.72 nm [PDI = 0.153] and 15.1±0.55 mV, (n=3). Similarly, 𝐶−𝑀𝑅𝑃 average size and ζ-potential were 1089±23.33 nm (n=3) and -14.2±0.25 mV (n=3). Tenofovir (TFV) …
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Effect of blueberry polyphenols on osteoclast
… markers for activity and differentiation. RAW 264.7 murine macrophage cells were cultured, differentiated into osteoclasts and treated with varying blueberry polyphenols doses. Levels of Tartrate Resistant Acid Phosphatases (TRAP), Nuclear factor of activated T-cells, cytoplasmic 1 …
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Cell Toxicity and Uptake of RRR-Alpha-Tocopheryl Polyethylene Glycol 1000 Succinate (TPGS) by Various Cell ines <em>In Vitro</em>.
… clinical applications. Cytotoxicity assays with RAW 264.7 and LNCaP cells incubated overnight with TPGS or α-TS at concentrations ≥ 12.4 μM suggest that α-TS is more cytotoxic than TPGS. Macrophages were found to be more sensitive than LNCaP cells when treated with similar concentrations of α-TS. …
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Role of an Adenylyl Cyclase Isoform in Alcohol's Effect on Cyclic AMP Regulated Gene Expression in Mammalian Cells
… immune cell lines endogenously expressing AC7, RAW 264.7 and BV-2, to further elucidate the role of AC7 in the effect of ethanol on cAMP regulated gene expression. To examine these objectives, time-lapse fluorescent resonance energy transfer (FRET) and cAMP accumulation assays were used to …
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Effect of Scutellaria Baicalensis on Reduction of Radiation/chemotherapy induced Inflammation
… changes, respectively. For in vitro study, RAW 264.7 cells were untreated or treated with S for 30min before exposure to 100ng/ml lipopolysaccharide(LPS). At the indicated time points following LPS stimulation, cells were harvested. To examine effect of S on transcription of the various …
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Fumarate Inhibits TNF-α Release
… TNF-α released from the mouse monocyte cell line RAW 264.7, with no effect on cell viability or reactive oxygen species (ROS) generation. Monomethyl fumarate (MMF) and DMF showed minimal effects on cell viability or ROS generation. We found that DMF at 10µM is anti-inflammatory with minimal …
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Development of versatile drug delivery strategies using PLGA nanoparticles
… syndrome. 02-8-NANA conjugated NP demonstrated RAW 264.7 monocyte cell targeting and internalisation. Co-incubation of these particles in RAW 264.7 cells induced inhibition of the LPS- stimulated release of pro-inflammatory cytokines. Furthermore, 02-8-NANA conjugated NP loaded with camptothecin …
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Multi-omic Approaches to Investigate the Inflammatory Response in Activated Macrophage Cells
… of cultured murine macrophages (RAW 264.7 cells) subjected to<br/>inflammatory stimuli. Cells were treated with immune modulators that either<br/>modelled bacterial infection (Kdo2-Lipid A) or reflected the signals produced by<br/>damaged or dying cells during clearance (ATP). …
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Biomolecules that impact phenotypic transitions in fibroblasts and macrophages
… CS-A, CS-B, CS-C, and CS-E were cultured with RAW 264.7 macrophages of five different phenotypes.</p>
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Utilização da quitosana como adjuvante as drogas antituberculoses e sua avaliação in vitro contra Mycobacterium tuberculosis
… do método utilizando brometo (MTT), em células Raw 264.7. Para os compostos estudados foram realizadas análises morfológicas e físico-químicas. Os resultados obtidos da CMI para o compósito QS/EMB 1:1 indicaram uma melhor atividade antimicobacteriana quando comparada aos demais compostos …
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The role of AcrA in the antibiotic resistance and virulence of Salmonella enterica serovar Typhimurium
… ability of S. Typhimurium to infect INT-407 and RAW 264.7 cells \(in vitro\) as previously published for AcrB and TolC mutants. The virulence defect of the mutants could not be rescued by addition of supernatant from an infection of INT-407 cells with SL1344 or addition of media conditioned by …
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Role of TRB3 in Glucose Enhancing Effect on LPS-induced NO Production
… 생성 증폭 반응에서 TRB3의 역할을 확인하고자 했다. 포도당 농도를 달리한 조건하에서 Raw 264.7 세포에서 TLR4 하위단계인 MyD88 경로 그리고 TRIF 경로의 specific agonist를 이용하여 iNOS, NO, NF-κB 그리고 IFN-β의 발현에 미치는 TRB3의 영향을 확인 하였다. 선행연구결과와 동일하게 고포도당 조건에서 LPS를 통한 NO생성은 증폭이 되고, 저포도당 조건에서는 감소하는 작용을 보였다. 이때 TRB3의 발현은 저포도당 조건에서 증가하였다. 염증반응을 매개하는 NO생성 조절인자인 …
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Studies of fluorescent imaging for mRNA detection in living cells
… PNA probes to detect the iNOS mRNA in living RAW 264.7 mouse macrophage cells. A probe constitutes of an antisense 23-mer fluorescein: FAM) labeled antisense PNA and a 17-mer Dabcylplus labeled complementary DNA was used. The fluorescence of the FAM was quenched when the two strands hybridized …
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