Global ETD Search
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Showing 1 to 20 of 20 for “"RAPD-PCR"”.
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RAPD-PCR DNA fingerprinting on sequential isolates of Histoplasma capsulatum from AIDS patients failing fluconazole therapy
This document only includes an excerpt of the corresponding thesis or dissertation. To request a digital scan of the full text, please contact the Ruth Lilly Medical Library's Interlibrary Loan Department (rlmlill@iu.edu).
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The optimisation of protocols for the extraction of nuclear DNA from animal bone forPCR.
… Polymorphic DNA- Polymerase Chain Reaction (RAPD-PCR) profiles of different species. A modified Chelex method was selected as the preferred extraction procedure and RAPD-PCR profiles were created for several species using a number of different primers. Individuality between closely related …
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Extra-pair paternity within the female-defense polygyny of the lizard, Anolis carolinensis: Evidence of alternative mating strategies
… a resident male's territory were collected and RAPD-PCR was used to determine the paternity of hatchlings. Of the 48 hatchlings from 26 females, resident territorial males fathered 52% of hatchlings; 15% were fathered by a male whose territory bordered that of the resident male and 21% were …
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Phenotypic and genotypic characterisation of Clostridium Difficile
… in the developed world. Hypervirulent strains (PCR ribotype 027) of C. difficile which produce enhanced levels of toxins have also been associated with other characteristics such as a greater rate of sporulation and resistance to fluoroquinolones. Infection due to C. difficile PCR ribotype 027 …
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Impact of a Historical Translocation on Populations of White-Tailed Deer Odocoileus virginianus
<p>Randomly amplified polymorphic DNA (RAPD) analysis was used to study the impact of a historical translocation event on populations of white-tailed deer in Georgia. The technique may also serve as a model by which management practices for other rare species can be studied. Populations from an …
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Development of Predictive Modeling for the Reduction of Escherichia Coli During the Curing Time of American Type Cheese
… of the bacterial isolates was checked by RAPD-PCR, which confirmed that the organism found in cheese milk were the same as the bacteria in the curing cheese. Enumerations of surviving cells showed several forms of declining curves, including classic first-order declines, lag phases and …
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Élelmiszer-biztonsági szempontból jelentős baktériumok kimutatása, PCR-alapú molekuláris azonosítása és tipizálása
… Munkám során célul tűztem ki olyan PCR-alapú molekuláris biológiai módszer kidolgozását, amelynek segítségével egy lépésben valósítható meg az E. coli faj, valamint a faj O157:H7 szerotípusú törzseinek kimutatása. Célom volt továbbá a két legjelentősebb termofil Campylobacter faj (C. …
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Effect of Pollution on Genetic Diversity in the Southern California Bight
… polymorphisms generated from genomic DNA by RAPD-PCR (Randomly Amplified Polymorphic DNA - Polymerase Chain Reaction). All populations exhibited a large amount of genetic diversity and were genetically similar to each other. However, several different measures of diversity indicated that for …
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Infektionen und Komplikationen nach Anlage einer Perkutanen Endoskopischen Gastrostomie (PEG)-Sonde : eine prospektive interdisziplinäre Studie
… nachgewiesen werden. Nach Typisierung durch RAPD-PCR konnte gezeigt werden, dass es sich in allen Fällen bei den Isolaten eines Patienten um genetisch identische Keime handelt. <br>Unsere Ergebnisse liefern erstmals genauere Erkenntnisse über Art und Herkunft der Erreger von Wundinfekten nach …
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Biochemical Separation of Geographical Strains of Plum Curculio, Conotrachelus nenuphar (Herbst) (Coleoptera: Curculionidae), and Evaluation of Olfactory Attractants in Virginia Orchards
… in orchards is difficult and often ineffective. RAPD-PCR assay was an effective method for separation of the geographical strains. Of the tested primers four, OPE 01, OPE 03, OPE 04, and OPE 07, gave 21 amplimers that are useful for distinguishing individuals from the univoltine and multivoltine …
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Genetic Characterization of Zambian Native Cattle Breeds
… and randomly amplified polymorphic DNA (RAPD) markers, respectively. A total of 100 animals, 25 from each of the four breeds associated with different tribes and region of Zambia, were used in the molecular analysis research. Additionally, 10 Holstein x Jersey crossbred animals were used …
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Combined Roles of Glandular-haired Alfalfa and Natural Enemies in Alfalfa Pest Managment in Virginia
… was surveyed at both study sites using RAPD-PCR to establish or eliminate the possibility that differences in parasitization levels between the Piedmont and southwestern regions could be attributed to the presence of different parasitoid strains. Most of the detected phenotypic variation …
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Intraspecific Variability within Globodera tabacum solanacearum and Selection for Virulence Against Flue-Cured Tobacco
… characterized by DNA fingerprinting using the RAPD-PCR technique. Reproducible differences in fragment patterns allowed similar differentiation of the isolates and species with each primer. Hierarchical cluster analysis was performed to illustrate the relatedness between nematode isolates and …
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Enumeration and discrimination of thermophilic bacilli in milk powder
… powder and their enumeration using real-time PCR methodology based on the 16S rRNA gene or the spoOA gene. Further modifications to these methods allow discrimination between live and dead cells and between spores and vegetative cells. The former is important because the majority of vegetative …
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Estudo de cepas de Yersinia pestis isoladas durante epizootia no Foco da Chapada do Araripe, Pernambuco, Brasil
… de Y. pestis por diferentes técnicas, como a RAPD-PCR, PCR-ribotipagem e RFLP-IS100, revelaram, na maioria das vezes, padrões genômicos idênticos entre as cepas, independentemente das fontes, procedência e ano. Recentemente, um estudo utilizando MLVA (análise de múltiplos locos do número …
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Maíz Bt : seguimiento de la resistencia de Sesamia nonagrioides (Lepidoptera: Noctuidae) y Ostrinia nubilalis (Lepidoptera: Crambidae) y efectos en artrópodos depredadores
… de S. nonagrioides mediante la técnica de RAPD-PCR, ya que el conocimiento de aquélla es importante para aplicar estrategias de manejo de la resistencia adecuadas. Se analizaron dos poblaciones del valle del Ebro, dos de la zona centro de la Península y dos de Badajoz. Los índices de …
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Clinical Application of S.salivarius 24SMBc in the Prevention of Recurrent Otitis Media in Paediatric Age
… per valutare la produzione di batteriocine e RAPD-PCR per distinguere il genotipo di S.salivarius 24SMBc da quello di altri ceppi appartenenti alla specie S.salivarius. Lo step seguente, finalizzato alla valutazione della colonizzazione di S.salivarius 24SMBc nelle alte vie respiratorie dei …
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REDUCING ANTIMICROBIAL RESISTANCE RISKS AND ANTIBIOTIC USAGE IN DAIRY FARMING: MICROBIOLOGICAL INSIGHTS AND ALTERNATIVE STRATEGIES
… pasture systems. The study revealed that RS-PCR analysis of S. aureus identified 12 genotypes within 8 clusters, with GTRI being the most prevalent (61%) and GTB at 14%. High penicillin resistance (69%), especially in CLR strains (88%), was observed, along with 51% resistance to amoxicillin …
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Isolasi dan karakteristik bakteri endofit dari umbi tanaman kentang (Solanum tuberosum. L) menggunakan primer PCR-RAPD
… endofit berdasarkan pola pita DNA dari teknik PCR-RAPD. Penelitian ini dilaksanakan tanggal 8 Januari 2012 sampai 27 Juli 2012 yang bertempat di Laboratorium Biomolekuler dan Genetika Universitas Islam Negeri Malang. Metode yang digunakan adalah deskriftif eksploratif. Dimana sampel bakteri …
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Untersuchungen zur Infektionsbiologie und Heritabilität von Infektionsparametern entomopathogener Nematoden bei Larven der Maikäfer (Melolontha spp.) sowie zur Charakterisierung der Nematoden durch DNA-Fingerprinting
… unterscheiden lassen, wurden die Methoden der RAPD-PCR (random amplified polymorphic DNA - polymerase chain reaction) und der ISSR-PCR (inter simple sequence repeats) angewandt. Die mittels der RAPD- bzw. der ISSR-PCR-Analyse ermittelte durchschnittliche genetische Distanz zwischen den Arten H. …