Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"RAPD markers"”.
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Use of RAPD markers in plant varietal development
DNA based molecular markers have played an important role in plant varietal development. With the discovery of polymerase chain reaction (PCR), very low or single copy sequences can act as molecular markers because during the PCR, DNA is multiplied to such an extent that it can be easily visualized …
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Applications of RAPD Markers in Soybean: Genetic Diversity and Linkage Analysis
To determine the efficacy of the pollen tube pathway transformation procedure plasmids carrying the bar and gus genes were applied to cut stigmas. Approximately 5000 seeds were produced from treated flowers. No plants derived from seeds produced by flowers treated with the plasmid carrying the bar …
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RAPD markers for ascochyta resistance, phylogenetic studies and cultivar identification In lentil
Random amplified polymorphic DNA (RAPD) markers were used in three genetic studies in lentil (Lens culinaris Medikus). The first study involved development of RAPD markers linked with genes for resistance to ascochyta blight, caused by Ascochyta fabae f. sp. lentis Gossen et al. Seventy F2-derived …
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Identification and Utilization of Diverse Germplasm for Soybean Yield Improvement
… selection for agronomic potential. Specific RAPD markers were identified that were present in the high yielding lines and their exotic parent, but not in the adapted parent. The yield increases over the adapted parent and unique RAPD markers provide solid evidence that exotic germplasm can …
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Genetic Diversity and Camptothecin Variation in Camptotheca Decaisne
… of Camptotheca and to identify taxa with RAPD markers; (2) to determine the camptothecin (CPT) variations within and among different populations and taxa, with tissues, season, and age; and (3) to determine the effects of environmental stress on the camptothecin production in Camptotheca. …
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Characterization of Resistance to Gray Leaf Spot [Pyricularia Grisea (Cooke) Sacc.] in Perennial Ryegrass and Tall Fescue
… (BSA) with random amplified polymorphic DNA (RAPD) markers to identify DNA markers associated with resistance to gray leaf spot. Although polymorphic bands were identified between the resistant and susceptible bulks, these markers were not confirmed on the entire population to be significantly …
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Factors affecting VA-mycorrhizal community structure in the Namib Dune Field: and the population biology of an ectomycorrhizal Basidiomycete: Suillus granulatus
… of S. granulatus single spore isolates using RAPD markers showed that a post-meiotic mitosis in the basidium produces heterokaryotic spores. Secondary homothallism provides an effective means for long distance dispersal, and may account for the broad geographic range of this ectomycorrhizal …
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Genetic diversity and interspecific relationships in Banksia L.f., (Proteaceae) / Tina Louise Maguire.
… techniques. Random amplified polymorphic DNA (RAPD) markers are assessed for their usefulness at various taxonomic levels within the genus. The results indicate a close relationship between Banksia and Dryandra, which are sister genera in the tribe Banksiae, family Proteaceae.
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Methods to Improve Marker Assisted Selection (Mas) for the Sugary Enhancer1 (Se1) Trait in Maize
SSRs and RAPDs from regions previously associated with kernel sugars on chromosomes 2, 3 and 6 were explored as additional mapping strategies. A new population of 308 F2:3 families was derived from near-isogenic inbreds IL451b (su1 Se1) X IL451b (su1 se1). The population was grown in replicated …
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Investigation into the use of molecular methods to distinguish between species of Caladenia subgenus Calonema
… first time. The analyses were performed using 17 RAPD and 10 ISSR primers on 30 taxa representing the three spider orchid groups (the dilatata, patersonii and reticulata groups) yielding 135 RAPD and 63 ISSR polymorphic markers. The average number of polymorphic markers produced from 17 RAPD and …
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Utilization of tissue culture methods and molecular markers for improvement of Solanum phureja Juz. & Buk.
… selection of genetically diverse parents using RAPD markers and to develop high yielding diploid potato germplasm that may be instrumental in new cultivar development were addressed. Genetic diversity among in vitro plantlets of S. phureja monoploids (which represent DM maternal genotypes) and …
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Embryogenic response of potato anther culture to colchicine
… among regenerated anther-derived plants based on RAPD markers, was also conducted. Anther culture of a hybrid between <i>Solanum chacoense</i> and <i>S. phureja</i> (clone CP2) was conducted with five colchicine treatments (0, 25, 50, 100, and 200 mg/L) for 24 h. Anthers containing late …
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Isolasi dan karakteristik bakteri endofit dari akar tanaman kentang (Solamun tuberosum L.) menggunakan primer penanda RAPD
… dengan penanda DNA. Teknik PCR dengan penanda RAPD (random amplified polymorphic DNA) telah dipergunakan secara luas dalam studi variasi genetik bakteri. Penelitian ini bertujuan untuk mengetahui hasil isolasi bakteri endofit dari akar tanaman kentang (Solanum tuberosum L.) dan mengetahui …
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Systematics and reproductive biology of the genus Morus L. (Moraceae)
… I applied randomly amplified polymorphic DNA (RAPD) markers and microsatellites. The Morus species were moderately ([Theta]II = 0.079; RAPD data) to highly differentiated genetically (F[subscript]ST = 0.233; microsatellite data). Analysis of genetic structure suggested interspecific gene flow …
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Studies of verticillium wilt and characterization of candidate verticillium wilt resistance genes in the mint species Mentha longifolia (L) Huds
… utilizing randomly amplified polymorphic DNA (RAPD) markers. The accessions were found to be diverse regarding all observed traits and the South African accessions in particular were shown to possess unique features. Most importantly, highly wilt-resistant and highly wiltsusceptible accessions …
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Genetic Characterization of Zambian Native Cattle Breeds
… and randomly amplified polymorphic DNA (RAPD) markers, respectively. A total of 100 animals, 25 from each of the four breeds associated with different tribes and region of Zambia, were used in the molecular analysis research. Additionally, 10 Holstein x Jersey crossbred animals were used …
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The relationship between fruit color traits and anthocyanin genes in diploid Fragaria
… mapped randomly amplified polymorphic DNA (RAPD) markers and morphological markers. The F3H gene was linked without recombination to the c locus in linkage group I, while the other five candidate genes mapped to different linkage groups. These results suggest that the wild type allele (C) of …