Global ETD Search
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Showing 1 to 20 of 95 for “"RAPD"”.
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Use of RAPD markers in plant varietal development
DNA based molecular markers have played an important role in plant varietal development. With the discovery of polymerase chain reaction (PCR), very low or single copy sequences can act as molecular markers because during the PCR, DNA is multiplied to such an extent that it can be easily visualized …
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Random amplified polymorphic DNA (RAPD) analysis of Bacillus sphaericus
… I have used random amplified polymorphic DNA (RAPD) fingerprinting to determine the phenetic relationships among 31 pathogenic and 14 nonpathogenic strains of B. sphaericus. DNA Bands in agarose gel migrating the same distance were verified as being homologous using PCR-generated probes made …
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Applications of RAPD Markers in Soybean: Genetic Diversity and Linkage Analysis
To determine the efficacy of the pollen tube pathway transformation procedure plasmids carrying the bar and gus genes were applied to cut stigmas. Approximately 5000 seeds were produced from treated flowers. No plants derived from seeds produced by flowers treated with the plasmid carrying the bar …
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RAPD markers for ascochyta resistance, phylogenetic studies and cultivar identification In lentil
Random amplified polymorphic DNA (RAPD) markers were used in three genetic studies in lentil (Lens culinaris Medikus). The first study involved development of RAPD markers linked with genes for resistance to ascochyta blight, caused by Ascochyta fabae f. sp. lentis Gossen et al. Seventy F2-derived …
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Molecular systematics and population genetics of Hypoxylon truncatum sensu Miller using RAPD
… using random amplified polymorphic DNA markers (RAPD). A simple, rapid method was developed to isolate DNA from fungal tissue. This method described involves treating ground mycelium with a lysis buffer containing SDS, followed by precipitation of contaminants using CsCl. Using DNA isolated by …
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Genotipificação de amostras sorotipificáveis e não sorotipificáveis de Actinobacillus pleuropneumoniae através de RAPD
… de A. pleuropneumpnoae, a técnica de RAPD foi avaliada na genotipificação de amostras sorotipificáveis e não sorotipificáveis do agente. Foram utilizados amostras ATCC dos 12 sorotipos e amostras dos sorotipos, 1, 3, 5a, 5b, 7, 11 e 12 isolados no Brasil. Os primers OPG e OPG-19, …
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The use of RAPD to detect genetic variation in populations of Liatris aspera Michx.
1 PDF file (viii, 54 pages)
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Isolasi dan karakteristik bakteri endofit dari akar tanaman kentang (Solamun tuberosum L.) menggunakan primer penanda RAPD
… dengan penanda DNA. Teknik PCR dengan penanda RAPD (random amplified polymorphic DNA) telah dipergunakan secara luas dalam studi variasi genetik bakteri. Penelitian ini bertujuan untuk mengetahui hasil isolasi bakteri endofit dari akar tanaman kentang (Solanum tuberosum L.) dan mengetahui …
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Isolasi dan karakteristik bakteri endofit dari umbi tanaman kentang (Solanum tuberosum. L) menggunakan primer PCR-RAPD
… berdasarkan pola pita DNA dari teknik PCR-RAPD. Penelitian ini dilaksanakan tanggal 8 Januari 2012 sampai 27 Juli 2012 yang bertempat di Laboratorium Biomolekuler dan Genetika Universitas Islam Negeri Malang. Metode yang digunakan adalah deskriftif eksploratif. Dimana sampel bakteri endofit …
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Morphometric, Isozyme and Random Amplified Polymorphic DNA (RAPD) : Analysis of SAROTHERODON MOSSAMBICUS Populations in Java, Indonesia
… of the 3 techniques (morphometric, isozyme and RAPD) in revealing differences within and among 9 Javanese populations. The data were also used to estimate the rate of divergence among the populations.</p> <p>Morphometric analysis showed significant size and shape differences among the …
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RAPD-PCR DNA fingerprinting on sequential isolates of Histoplasma capsulatum from AIDS patients failing fluconazole therapy
This document only includes an excerpt of the corresponding thesis or dissertation. To request a digital scan of the full text, please contact the Ruth Lilly Medical Library's Interlibrary Loan Department (rlmlill@iu.edu).
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Investigation into the use of molecular methods to distinguish between species of Caladenia subgenus Calonema
… first time. The analyses were performed using 17 RAPD and 10 ISSR primers on 30 taxa representing the three spider orchid groups (the dilatata, patersonii and reticulata groups) yielding 135 RAPD and 63 ISSR polymorphic markers. The average number of polymorphic markers produced from 17 RAPD and …
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Development of DNA markers and the genetic analysis of seven populations of the freshwater mussel Lampsilis radiata siliquoidea (Bivalvia: Unionidae) in north central Minnesota
… Minnesota. Random amplified polymorphic DNA (RAPD), heteroduplex, and single-stranded conformational polymorphism (SSCP) analyses were performed using nuclear DNA markers. We assayed the populations for variation using a single repetitive 18 base pair RAPD primer and two loci that flanked …
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Control of barley yellow dwarf virus (BYDV) in oat and wheat and identification of a RAPD marker associated with BYDV tolerance in oat
Populations of F$\sb2$ and F$\sb3$ plants from intraspecific spring oat (Avena sativa L.) crosses were grown in the greenhouse using a modified single-seed descent method with 100 plants per 15 cm pot. Subpopulations were inoculated with the BYDV-PAV-IL strain (1) in the F$\sb2$ generations only, …
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Phylogenetic and antibiotic resistance variance amongst mastitis causing E. coli : the key to effective control
… method (Random Amplification of Polymorphic DNA; RAPD) and an antibiogram based method, were used. A dairy farm milking approximately 1000 Holstein cows in the Darling area, Western Cape Province, was selected for this study. The study was conducted over a period of 48 months and mastitis samples …
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