Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"RAD-seq"”.
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Linkage mapping in tetraploid blackberry (Rubus spp.) using high-throughput genomic sequencing and restriction-site associated DNA sequencing (RAD-seq)
… among homologous and homeologous chromosomes. Traditional techniques for studying linkage in tetraploid species, including Rubus, have made use of limited numbers of scorable markers – such as expressed sequence tag simple sequence repeats (EST-SSRs) or amplified fragment length polymorphisms …
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Population Genetic Structure of Ixodes pacificus Ticks and Detection of Their Pathogens In-Silico
… public health crises. The movement and subsequent adaptation to the new environment by ticks can be modeled using genetic information and can be used to inform public health agencies of the risk of encountering ticks. Using Restriction-site DNA sequencing (RAD-seq) (Chapter 2), tick …
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Understanding gender determination in dioecious Amaranthus weeds palmer amaranth (Amaranthus palmeri) and waterhemp (Amaranthus tuberculatus)
… work, we use restriction-site associated DNA sequencing (RAD- Seq) to investigate the genetic basis of sex determination in A. tuberculatus and A. palmeri. For each species, approximately 200 plants of each sex were sampled and used to create RAD-Seq libraries. The resulting libraries were …
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Assessing the genetic diversity and structure of spotted hyena (Crocuta Crocuta) populations across Africa
… 55 whole genome and restriction site-associated sequences were analysed using Heterozygosity, PCA analysis, Admixture proportions, Phylogenetic analysis and D-statistics. The results showed that the mean heterozygosity of spotted hyena was 0.00094 (RAD-seq) and 0.0011 (WGS) ranging from 0.0006 to …
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Genetic Diversity of Blue Wildebeest (Connochaetes taurinus) in the Liuwa Plain of Zambia
<p>Advances in DNA sequencing are enabling researchers to identify and genotype large sets of single nucleotide polymorphisms (SNPs) in non-model organisms to answer questions concerning ecology, evolution, and conservation. Analysis of SNP allele frequencies can provide insight into the amount of …
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Landscape genomics of tropical high altitude plant species
… patterns that will be observed as a consequence depend on explicit geographical features, such as topography and latitude. Over the first decades of phylogeography, the effect of climate history and geography on species genomes was examined at low resolution with DNA sequences and other …
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Sex chromosome evolution of papaya: Dynamic structural and expression changes and identification of associated traits
… used to create restriction-site associated DNA sequencing (RAD-seq) libraries. A total of 228 RAD-seq markers were mapped to 9 major and 2 minor linkage groups. Previous studies have shown that the Y chromosome experiences severe recombination suppression along the sex determining region. The …
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Genomic and Phenotypic Analyses of Polychaete Sibling Species <i>Platynereis dumerilii</i> and <i>Platynereis massiliensis</i> in Relation to Ocean Acidification
… carbon dioxide emissions and the subsequent uptake of CO<sub>2</sub> by the sea, is leading to a decrease in the pH of the oceans, a process known as Ocean Acidification. One of the main challenges of the current research on climate change is to determine how marine species respond to …
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Genetic and morphometric variation of two focal snapper species (Lutjanus bohar and Lutjanus gibbus) in the Chagos Archipelago
… might adapt to environmental change and degradation. Three separate approaches were used to carry out this investigation: 43 individual L. bohar were screened for genetic variation at the cytochrome c oxidase subunit 1 (CO1) mitochondrial DNA (mtDNA) gene. Results show overall high mtDNA …
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Genomic characterizations of Xanthomonas cucurbitae and using comparative genomics to predict novel microbe-associated molecular patterns in Xanthomonas
… quality X. cucurbitae genome assembly, an RNA-seq analysis was carried out to assess virulence characteristics of the pathogen. By analyzing the X. cucurbitae transcriptome, we observed behavioral changes between nutrient-sufficient and host-mimicking conditions, as well as the upregulation of …
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The colonisation of canola crops by the diamondback moth, Plutella xylostella L., in southern Australia
… in Australian P. xylostella was assessed using RAD-seq, first using nine field and laboratory populations to refine methods, then in 59 field populations collected throughout southern Australia in two separate years from wild and cultivated brassicas. A statistically powerful genome-wide SNP …
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The winds of change: A study of post-glacial dispersal in pacific-northwest conifers
… mertensiana), across the PNW using the new RAD-Seq Genotyping-by-Sequencing method and combined the results with new paleoecological data from the same region to reconstruct the past population dynamics that resulted in their current distribution. The third principal chapter (Chapter 4) aims …
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Adapting to salinity: The effects of salinity on population structure and offspring survival in Lucania parva
… related to osmoregulation. In chapter 2, I used RAD-Seq data from 10 populations across Florida to examine the levels of population structure between freshwater and saltwater populations and the effects of distance on population-wide FST. Here, I found good evidence that differences in salinity …
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Introgression, Population Structure, and Systematics of the Sphagnum capillifolium complex
… capillifolium complex using whole genome resequencing data. The species tree phylogeny was generally well supported but phylogenetic discordance among genomic regions was prevalent, especially at nodes in the backbone. Alternative topologies for each of the backbone nodes were not random, …
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Early patterns of Andean diversification, genomic and color patterns variation across the Metallura tyrianthina complex of the Northern Andes
… mediante un muestreo reducido del genoma (NextRAD) y valoraciones cuantitativas de coloración. Esto nos permitió encontrar evidencia significativa de procesos de divergencia temprana en presencia de flujo genético. En primer lugar, la evidencia mostró que los ciclos glaciales del Cuaternario …