Global ETD Search
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Showing 1 to 20 of 30 for “"R gene"”.
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Maize R gene Rxo1 Confers Disease Resistance on Pepper and Nicotiana benthamiana
… destructive diseases on pepper. Due to limited genetic resistance resources in host species, a promising strategy for controlling BS disease is to transfer nonhost disease resistance (R) genes from other plant species into pepper plants to confer broad-spectrum and durable resistance. A maize R …
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Investigating R gene evolution by meiotic recombination using synthetic gene clusters in Arabidopsis
Plant gene families organized as linked clusters are capable of evolving by a process of unequal crossing-over. This results in the formation of chimeric genes that may impart a novel function. However, the frequency and functional consequences of these unequal cross-over events are poorly …
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Characterization of a novel tetracycline resistance gene from the Bacteroides transposons Tn4351 and Tn4400
A tetracycline resistance ($\rm \sp{*}Tc\sp{r}$) gene that was originally found on two Bactetoides transposons, Tn4351 and Tn4400, confers resistance on Escherichia coli, but only when the cells are grown aerobically. I first showed that the product of the $\rm \sp{*}Tc\sp{r}$ gene is a 44 kDa …
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Dissecting Transcriptional Regulation of Rpp8 in Arabidopsis thaliana
… plant growth. However, little is known about R gene regulation under defense-inducing conditions. The interaction between the oomycete Hyaloperonospora parasitica and the model plant Arabidopsis thaliana provides an excellent model system to explore R gene regulation. My research focuses on …
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Rice Blast Disease in the U.S. and Africa: Determination of Pathogen Diversity and the Identification of Resistance Genes for Disease Management
… as determination of variation in the avirulence gene AVRPiz-t in isolates from Africa. Also, evaluation of blast resistance genes in the interspecific rice germplasm “New Rice for Africa” NERICA was done using F2 progeny of the cross of U.S. susceptible cultivar M204 and NERICA 12. The U.S. …
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Inheritance of virulence in the root rot pathogen Phytophthora sojae
… avirulence (Avr) and host resistance (R)-genes determine the disease outcome. The Avr3a mRNA transcript level is variable among P. sojae strains and determines virulence towards the R-gene Rps3a. To study the inheritance of virulence, genetic crosses and self-fertilizations were performed. …
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A Central Regulatory Locus Controls Tetracycline Regulated Functions on a Bacteroides Tetracycline Resistant Conjugal Chromosomal Element
The genes necessary for regulated Plf activity on the Tc$\sp{\rm r}$ element were localized to an 8 kbp region. This region was known to contain the Tc$\sp{\rm r}$ gene, tetQ, which had already been sequenced. Sequencing of an additional 5 kbp downstream of tetQ has revealed two additional genes. …
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Analyzing the Importance of Phosphorylation and Ubiquitination of Amino Acids in the Enhancer of Rudimentary Protein, ERH
The enhancer of rudimentary gene, e(r), has been found to posses certain amino acids with the potential for phosphorylation or ubiquitination. All of these amino acids are highly conserved among species. It is possible that any of these sites might play a role in Enhancer of Rudimenary Homolog …
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Cucurbit Downy Mildew (Pseudoperonospora cubensis): Cucumber Resistance
… conducted, comparing the relative expression of genes encoding a basic PR-1 protein, a cytosolic ascorbate peroxidase protein and three resistance (R) gene proteins, in nineteen cultivars. All of the selected genes were analyzed using real-time PCR. The relative expression levels of the R-genes …
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REGULATION OF DEFENSE RESPONSES MEDIATED BY BON1 AND BAP2 IN ARABIDOPSIS THALIANA
… these threats could enable the production of genetically modified plants with enhanced innate immunity without the use of chemicals to which insects and microbes are renown for developing resistances to. Plants have three main defense mechanisms to guard against bacteria: physical tissue …
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Dissection of Regulatory Networks Mediating Resistance and Susceptibility of Arabidopsis thaliana to the Downy Mildew Pathogen Hyaloperonospora parasitica
… triggered by the Arabidopsis thaliana R gene, RPP7, which encodes a coiled-coil nucleotide binding-leucine-rich repeat (CC-NB-LRR) protein that activates race-specific resistance to the downy mildew pathogen, Hyaloperonospora parasitica (Hpa). Previously-published genetic epistasis tests …
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Genetic characterization of a dominant susceptible Rpp1 allele and analysis of quantitative resistance to Asian soybean rust
… to P. pachyrhizi has been identified as single R gene-conferred, and seven Rpp (Rpp1-Rpp7; Resistance to Phakopsora pachyrhizi 1-7) gene loci have been identified and mapped (Hartman et al. 2012). Although multiple Rpp genes have been mapped, none have been cloned and validated, and the molecular …
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Data Mining for Selected Genes of Agronomic Interest from the Oil Palm Genome Using a Comparative Genomics Approach
Data mining of genes related to agronomic traits and transcription factors in plant genomes is essential to provide information on target genes for breeders. Taking advantage of the availability of completed genome sequences and the gene model available in a public database, the comparative genomic …
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Serotype Association and Regulation of the Hyaluronate Lyase Gene of <i>Streptococcus pyogenes</i>
<p><em>S. pyogenes</em> expresses many virulence factors, controlled by a complex regulatory network. These include hyaluronate lyase, an enzyme that degrades hyaluronic acid, a major component of the human extracellular matrix. Paradoxically, hyaluronic acid is also the sole component of the …
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Genes and mechanisms in Arabidopsis innate immunity against Leptosphaeria maculans
… was dependent on TIR-NB-LRR resistance genes (RLM1Col and RLM2Ler), which were independent of signalling components previously associated to all TIR-NB-LRR resistance genes. RLM1Col was found to be responsible for L. maculans induced callose depositions. A segregant analysis of the …
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Identification of <i>Phytophthora capsici</i> nuclear effector targets to bolster host immune signalling and resistance pathways
… responses. To enhance plant immunity and to generate new resistances in crops, it is essential to develop new strategies.<br/><br/>Synthetic biology approaches emerged as a tool to bolster plant immunity. There is a great deal of evidence that modification of plant proteins can affect …
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Molecular biology and biochemistry of brain tumours
… releasing hormone (GHRH) receptor (GHRH-R) gene was also determined. Finally, defects in the ß-catenin gene, an important component of the developmental pathway, in a sub-set of craniopharyngiomas were investigated. Reverse transcription-polymerase chain reaction (RT-PCR), restriction …
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Dynamic properties of the constitutive centromere associated network of proteins
Ensuring the proper transmission of genetic material across generations is fundamental to the propagation of cellular life. In each mitotic cell division, a series of highly regulated processes ensure that the genetic material of a dividing cell is properly duplicated and segregated to produce two …
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Using Synthetic Gene Clusters to Model Resistance Gene Evolution by Meiotic Recombination in Arabidopsis thaliana
… of constitutively expressed resistance (R) genes. R genes recognize pathogen gene products as signals of invasion. We are interested in how plants evolve R genes to keep pace with rapidly evolving pathogen populations. The mechanisms that drive the evolution of new R genes are poorly …
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Amplification of the DFR1 gene in Saccaromyces cerevisiae
… employed to study amplification of t,he DHF'R gene DFB,1 ) in Sa<,:;charoillYCB§. .Q~~Yi...S!i<;1~. . This system consists of a series of yeast strains containing a casset,te which encodes t he yeast, D..ERl gene ttghtly linked tjO a f usion of the yeast 1EU2. regulat,ory region wi tJ1 the LAQZ …
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