Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 4 of 4 for “"Quench flow"”.
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Conformational Analysis of Disordered Proteins Using H/D Exchange Mass Spectrometry
… when manual pipetting is used to add label and quench buffers to protein samples. A quench flow apparatus was used to extend the lower time scale limit of exposure times to 42 milliseconds. When CBP and ACTR were labeled using the quench flow apparatus, analyses revealed subtle exchange …
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The molecular basis of nucleotide recognition for T7 DNA polymerase
… step with a rate comparable to kpol measured by quench-flow experiments. Correct nucleotide binding caused an increase in fluorescence, suggesting that the conformational change of the fingers domain delivers incoming nucleotide to a misaligned status even for a correct nucleotide with each of …
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The influence of heat transfer limitations on the properties of PET yarn produced by melt spinning
… form the filament bundle. During this process a quench air stream is blown across the filament bundle to aid in cooling the molten polymer. Here, heat transfer limitations may cause inter-filament property variations, which will adversely affect the quality of the yarn. This thesis focuses on the …
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Biochemical mechanisms for selectivity governing RNA and DNA replication
Enzymes that catalyze DNA and RNA replication are biologically important and provide examples to understand enzyme specificity as the correct substrate and alternate substrates are well known. The DNA polymerase from bacteriophage T7 has been a model system for understanding high-fidelity DNA …