Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 7 of 7 for “"Quantitative reverse transcription polymerase chain reaction (qRT-PCR)"”.
-
Analysis of the Immunomodulatory Properties of Mycobacterium Cell Wall Fraction on the Cytokine Production of Peripheral Blood Mononuclear Cells of Healthy Dogs
… enzyme-linked immunosorbent assays (ELISA) and quantitative reverse-transcription polymerase chain reaction (qRT-PCR) for quantifying cytokine protein and mRNA transcripts, respectively. PBMCs were isolated from eight healthy dogs and cells were cultured with MCWF. A significant increase of …
-
FEEDLOT PERFORMANCE AND IMMUNOLOGY OF BEEF STEERS FED A LOW VITAMIN A DIET AND SELECTED FOR ADH1C GENOTYPE
… (PBMC) proliferation and stimulation assays, and quantitative reverse transcription polymerase chain reaction (qRT-PCR) examination of cytokine expression were conducted and compared. Vitamin A did not affect any of the measured parameters, and any significant (P < 0.05) pen and genotype effects …
-
Investigation of Cellular Responses Activated by Mechanical Compression in Equine Chondrocytes: Device Design, Construction and Testing
… outlined for companion use with our device. Quantitative reverse transcription polymerase chain reaction (QRT-PCR) revealed changes in expression of collagen II and matrix metalloproteinase-3 under dynamic compression for 24 hr. Equine chondrocytes compressed for 48 hr showed lower PG …
-
Osteogenesis During Early Healing Around Titanium and Titanium Zirconium Implants: Immunohistochemistry and mRNA Expression of Bone Markers in Miniature Pigs
… of bone healing around dental implants in vivo. Quantitative reverse transcription polymerase chain reaction (QRT-PCR) is a method that can be utilized to evaluate the early stages of peri-implant wound healing at molecular level by analyzing the level of gene transcription. In addition, the …
-
3D Bioprinting Meniscus for Future Tissue Replacement in Osteoarthritis Affected Knee Joints
… assay, immunohistochemical analysis, and quantitative reverse transcription polymerase chain reaction (qRT PCR). The work also comprised an in vivo assay of 3D bioprinted constructs transplanted subcutaneously in mice to enable the evaluation of tissue formation in a realistic milieu. …
-
Repeated Zolpidem Treatment Effects on Sedative Tolerance, Withdrawal, mRNA Levels, and Protein Expression
… field. Messenger RNA levels were measured by quantitative real time quantitative reverse transcription polymerase chain reaction (qRT-PCR) and protein expression was measured by western blot. The surface and intracellular proteins were separated using bissulfosuccinimidyl suberate (BS3) …
-
Functional genomics of plant compensatory responses
… of plant compensatory responses using suite of quantitative and molecular techniques. As a first step, QTL mapping was combined with results from a microarray analysis to identify potential candidate genes viz., G6PDH1 and invertase. These genes were then characterized using recombinant DNA …