Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 39 for “"Quantitative mass spectrometry"”.
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Analysis of Human Protein Complexes by Quantitative Mass Spectrometry
… chromatography (SEC) combined with tandem mass spectrometry. Combining these approaches has enabled an in-depth analysis of protein complexes in U2OS cells, including those involving post-translationally modified proteins and protein isoforms. The data presented in this thesis provide a …
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Protein Set for Normalization of Quantitative Mass Spectrometry Data
Mass spectrometry has been recognized as a prominent analytical technique for peptide and protein identification and quantitation. With the advent of soft ionization methods, such as electrospray ionization and matrix assisted laser desorption/ionization, mass spectrometry has opened a new era for …
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Picosecond Infrared Lasers (PIRL): Applications in Biodiagnostics and Towards Quantitative Mass Spectrometry
… exploited for a new form of spatially resolved mass spectrometry with the prospect of near instant biodiagnostics with mass spectroscopic sensitivity. The ion source for imaging mass spectrometer was based on PIRL ablation with an electrospray pick up source to entrain the ablated species. This …
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An investigation of the gas phase thermolysis of hexaborane (10) by quantitative mass spectrometry
… a gaseous reaction mixture to be sampled by a mass spectrometer. Samples from the reaction mixture flow into the spectrometer under a regime of viscous flow in an excess background of helium. This technique preserves the composition of the sample as it passes from the reaction vessel into the …
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Quantitative mass spectrometry-based approaches for characterizing the immunopeptidome and tyrosine phosphoproteome in cancer
… analyses stem from innovations in the field of mass spectrometry (MS), an analytical method which allows for the sequencing, identification, and quantification of peptides and proteins in complex biological mixtures. MS enables a molecular and systems-wide understanding of the cell state, …
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Quantitative mass spectrometry analysis of the early signaling dynamics of the epidermal growth factor receptor
… targeted inhibition of Src and P13K create a quantitative mechanistic explanation for EGFR signaling. Lastly, inhibition of the network with clinically relevant tyrosines kinase inhibitors reveals temporally distinct effects of inhibitors in early signaling. Combination of broad kinase and …
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A flexible and versatile framework for statistical design and analysis of quantitative mass spectrometry-based proteomic experiments
<p>Quantitative mass spectrometry (MS)-based proteomics is an indispensable technology for biological and clinical research. As the proteomics field grows, MS-based proteomic workflows are becoming more complex and diverse. The accuracy and the throughput of the MS measurements and of the signal …
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Development and Application of a quantitative Mass spectrometry based Platform for Thermodynamic Analysis of Protein interaction Networks
… protein -protein interactions are generally not quantitative and largely limited to certain types of interactions such as binary and strong binding interactions. They also have high false-positive and false-negative rates. Described here is the development of and application of mass …
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Identification of a novel metastasis enhancer, CDCP1, and analysis of its functions during melanoma metastasis
… may contribute to tumor metastasis. I applied quantitative mass spectrometry technology to first identify plasma proteins that are expressed at different levels in melanoma cells with high versus low metastatic abilities. Using SILAC (stable isotope labeling with amino acids in culture) coupled …
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Tissue- specific DNA methylation profiles in newborns
… DMR and H19 was measured in all tissues using quantitative mass spectrometry. ANOVA testing showed tissue-specific differences in DNA methylation of IGF2 DMR (p value 0.002) and H19 (p value 0.001) mainly due to a higher methylation of IGF2 DMR in Wharton jelly (mean 0.65, sd 0.14) and a lower …
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Differential expression profiling of proteomes of pathogenic and commensal strains of Staphylococcus aureus using SILAC
… expressed proteins was carried out using a quantitative mass spectrometry (MS)-based proteomic approach, known as stable isotope labeling of amino acids in cell culture (SILAC). Four commensal and pathogenic strains each were grown in the SILAC minimal media (RPMI 1640), containing light …
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Innate Immune Sensing and Signaling of Cytosolic DNA
… activation. Through biochemical purification and quantitative mass spectrometry, we identified the enzyme that synthesizes cGAMP in a DNA dependent manner. This enzyme, which we named cyclic GMP-AMP synthase (cGAS), turned out to be the long sought-after cytosolic DNA sensor. Structural and …
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The identification of the mTOR-regulated phosphoproteome and a mediator of feedback inhibition to P13K-Akt
… we defined the mTOR-regulated phosphoproteome by quantitative mass spectrometry and characterized the primary sequence motif specificity of mTOR using positional scanning peptide libraries. We found that the phosphorylation response to insulin is largely mTOR-dependent and that mTOR regulates the …
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Enabling tools for biological analysis : technologies for the study of protein dynamics, detection and interaction
… integrated the identification of proteins with quantitative mass spectrometry (SILAC). Using these arrays we have probed changes in the phosphorylation state of cells in response to activation of the Erb1 and Erb2 receptors. Using our microarray platform we were able to further probe the …
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The oocyte-to-embryo transition : regulation of oocyte maturation and egg activation in Drosophila
… processes of maturation and activation through quantitative mass spectrometry. Pairwise comparison of protein levels between pre- and post- maturation oocytes (stage 10 vs. stage 14 oocytes) or pre- and post-activation eggs (stage 14 vs. unfertilized eggs) identified candidate proteins up- and …
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Building the Human Argonaute 2 Interaction Network
… through immunoprecipitation and a novel semi-quantitative mass spectrometry technique known as the Normalized Spectral Index Method (SINQ). Stringent screening of mass spectrometry results identified interactions with the TRNC6 proteins, and AGO3. Most cytoplasmic interacting partners were …
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Developing a novel Drosophila model to study Menin biological function
… larval brain and adult head lysates. Label-free quantitative mass spectrometry of optimised immunoprecipitates identified Menin as the most enriched protein in the larval brain comparison, with Drosophila orthologues of the majority of established mammalian Menin partners detected in the dataset. …
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INTEGRATION OF REDOX-REGULATED SIGNALING AND METABOLISM IN HEAD AND NECK CANCER
… differences between SCC-61 and rSCC-61 using quantitative mass spectrometry and complementary validation assays. We identified broad changes in signaling and metabolic pathways indicating mesenchymal-to-epithelial transition, increased DNA repair, and increased expression of antioxidant …
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Analyzing Sumo-2/3 Modification In Regulation Of Breast Cancer Progression And Mitotic Chromosome Segregation
… epitope peptide elution (SUMO-2/3-ePIPE) and quantitative mass spectrometry, 66 SUMO-2/3 targets were identified, of which 15 targets are upregulated/downregulated in 66cl4 compared to 168FARN and 45 substrates are linked to cancer. About ten of these substrates were validated using in vitro …
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The Role of the Mammalian Target of Rapamycin (mTOR) in Cytotoxic T Lymphocytes
… in CTL.<br/>We thus utilised high resolution quantitative mass spectrometry to define the mTOR regulated CTL proteome and map the abundance and isoform expression of more than 6700 proteins in CTL. The data provide unbiased analysis of how mTORC1 reprograms the transcriptional and proteome …
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