Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 140 for “"Quantitative Real-Time PCR"”.
-
Quantitative Real-Time PCR Analysis of Colchicine Pathway and Rhizome Developmental Genes
… and rhizome development, respectively. Using quantitative real-time polymerase chain reaction (qRT-PCR), a comparative analysis of gene expression in G. superba may reveal differential gene expression of selected candidate genes. Reference genes were selected and analyzed for normalization of …
-
Chemoreceptors From the Hawk Moth Manduca Sexta L
… in Genbank databases and the results of RT-PCR and quantitative real-time PCR experiments show MsOr1 is expressed only in male antennae suggesting a male-specific function for this receptor. In situ hybridization revealed MsOr1 was expressed exclusively in a single cell associated with …
-
MicroRNAs: Tissue Expression and Role in 3T3-L1 Pre-Adipocyte Differentiation
… microarrays and confirmed by northern blot and quantitative real time PCR. Several microRNAs, including let-7, were up-regulated at the late stage of 3T3-L1 adipogenesis. let-7 expression specifically increased during the late stage of 3T3-L1 differentiation and ectopic introduction of let-7 in …
-
The Role of Exogenous Somatotropin, Ovariectomy and Extracellular Matrix in Bovine Mammary Gland Development
… for histological staining or snap frozen for quantitative real-time PCR. We examined 13 genes that work in conjunction with the extracellular matrix to regulate mammary proliferation and morphogenesis. Administration of GH, while impacting composition of MFP, had no effect on expression of the …
-
Expression Of Nicotinic Acetylcholine Receptor mRNA As a Function Of Age In Whole Hippocampus Preparations From Wistar Rats
… transcription reaction, then analyzed with quantitative real-time PCR to determine the relative levels of the mRNAs the cells were expressing at the age when the samples were obtained. The relative expression of the levels of RNA were then compared across age groups by subunits and across …
-
Promotion of Hair Cell Fate by Atoh1 and MicroRNA-183 Family
… miRNA-183 family is known to be expressed at the time of hair cell differentiation, and mutations in miR-96 (one member of the family) can cause deafness both in mouse and human. I hypothesize that combining Atoh1 with miR-183 family is efficient in promoting hair cell fate. Our goal is to …
-
The characterisation of calpain-like proteins in Trypanosoma brucei
… and bloodstream-stage of T. brucei using quantitative real time PCR and identified the differential expression of some of the calpain genes. We also present a comprehensive analysis of the subcellular localisation of selected members of this protein family in trypanosomes. Here, of …
-
The Effects of Angiogenic Growth Factors on Mesenchymal Stem Cell Proliferation and Differentiation to Vascular Cell Fates
… were determined by cell cycle analysis, quantitative real-time PCR (RT-qPCR) analysis, and flow cytometry. bFGF significantly stimulated MSC proliferation and inhibited expression of mural cell markers, with no apparent effect on endothelial marker expression. VEGF alone or in combination …
-
Sex Determination And Differentiation In The Common Snapping Turtle - A Reptile With Temperature-Dependent Sex Determination
… with TSD. </p> <p> I used differential-display PCR to clone a candidate gene involved in TSD, the cold-inducible RNA binding protein (Cirbp). The temporal and spatial patterns of Cirbp mRNA and protein expression during and after sex determination were determined using quantitative real-time …
-
Prevalence of Batrachochytrium Dendrobatidis in an Amphibian Communities of Central Texas and Tamaulipas, Mexico
… of the pathogen was assessed by using a Taqman quantitative real time PCR (qPCR) assay in a highly sensitive approach to detection. Interestingly, there is a low prevalence for Bd in Central Texas in comparison with previous studies conducted in this area. All samples tested from Tamaulipas were …
-
Hypoxic Stress Induced Gene Modulation in Medaka (Oryzias latipes) and Xiphophorus maculatus
… to profile hypoxia. In this study, we employed quantitative Real-Time PCR (qRT-PCR) to assess modulated gene expression in four tissues (brain, liver, gill, and fin) of Oryzias latipes and Xiphophorus maculatus. We analyzed six potential gene targets that had previously been shown in microarray …
-
Microbial Source Tracking of Bacteroides to Identify Human and Bovine Fecal Loading in McDaniel Lake
… identification of fecal sources. Using quantitative real-time PCR (qPCR), human-associated and bovine-associated Bacteroides DNA, as well as total Bacteroides DNA can be quantified and concentrations of fecal contamination in lakes and streams can be determined. Amplification control …
-
Quantitative analysis of individual flue-cured tobacco seed tissues reveals Tobacco mosaic virus infection in embryos
… sample and synthesized into cDNA for analysis. A quantitative real-time PCR (RT-qPCR) assay was developed to quantify viral titers in each component, while endpoint PCR confirmed RT- qPCR results and established a threshold viral cycle (Ct) value. Endpoint PCR results revealed viral accumulation …
-
An investigation of Fusarium verticillioides infection in maize using physiological and molecular approaches
… infection and showed that leakage increased over time in both control and infected samples with no significant difference observed between the two groups. Biochemical characterisation by analysing superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR) antioxidant enzymes showed …
-
Calcium homeostasis in Escherichia coli
… in calcium transport, and (iii) performing quantitative analysis of a specific subset of 15 genes to elucidate their possible involvement in calcium transport and homeostasis. The results of the systematic research approach corroborate existing knowledge regarding the involvement of genes …
-
Attachment Ability and Melanoma Inhibitory Activity mRNA Expression Level Changes in Murine B16-F10 Melanoma Cells Post Nanosecond Electric Pulses
… levels were measured using the cDNA temple via quantitative real-time PCR, with β–actin as the internal control. The experiment was repeated three times (n=3). The results show that a single pulse did not affect the cell attachment ability, cell morphology, and the MIA mRNA expression levels …
-
High throughput profiling of transcription factors involved in soybean root growth under water deficit
… 2 of water-stressed and well-watered roots using quantitative real-time PCR. 186 root- and stress-related TFs were selected to identify their specific expression patterns in root regions 1 and 2 of well-watered and water-stressed soybean seedlings at four time points (5h, 12h, 24h, and 48h) after …
-
Floral induction in Brachypodium distachyon
… model system to study the biology of temperate cereals. The present work investigates the “mechanisms” of flowering time in Brachypodium (community standard line Bd21) by analyzing a group of core genes, mostly transcription factors, generally known to be functionally involved in the determination …
-
Prediction of age from DNA
… between 1 and 96 years old was measured using quantitative real time PCR. The causes for the presence of large variation in telomere lengths in the population were further investigated. The age prediction accuracies were low even after dividing samples into non-related Europeans, males and …
-
Plasticity of the heat shock response and development of thermotolerance during embryonic development of Lake Whitefish (Coregonus clupeaformis)
… of heat shock and post-heat shock recovery time can modulate the heat shock response to a subsequent more severe stressor; 2) determine if this interplay is the same at different embryonic stages; and 3) assess whether transient heat shocks confer protection to the embryo. These objectives …
Page 1 of 7