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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.

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Showing 1 to 20 of 121 for “"Quantitative Polymerase Chain Reaction"”.

  1. Evaluation of a Paratuberculosis Quantitative Polymerase Chain Reaction Assay with Microscopic Correlation

    Tyler, Ronald Dale Jr.Virginia Tech2012

    … were tested with a commercial paratuberculosis quantitative polymerase chain reaction (qPCR) assay and had a sensitivity of 92%. To determine assay specificity, 21 FFPE tissues from animals without gastrointestinal disease combined with 13 FFPE tissues from non-ruminant animals (frog, dove, …

    vt Repository record

  2. Determination of soybean cultivar resistance to soybean cyst nematode with quantitative polymerase chain reaction

    Lopez Nicora, Horacio D.University of Illinois at Urbana-Champaign2010

    … of soybean roots by H. glycines with real-time quantitative Polymerase Chain Reaction (qPCR), a prelude to differentiation of resistance levels in soybean cultivars. Two experiments were conducted. In the first one, a consistent inoculation method was developed using to provide active …

    uiuc Repository record

  3. Optimization of automated immunomagnetic separation for the detection of escherichia coli 0157: H7 by quantitative polymerase chain reaction

    Khan, AyeshaUniversity of Central Lancashire2006

    … Carriage of the organism through the food chain can result in contamination of raw meat, dairy products, crops and produce during production. Numerous outbreaks of the diseases have made it essential to produce detection methods that are rapid, sensitive and specific, especially as the …

    cent-lancashire Repository record

  4. Verifying the Deletion of Growth Hormone Receptor Using a Quantitative Polymerase Chain Reaction at the mRNA Level in Tissue-Specific GHR-/- Mice

    Wang, XinyueOhio University2012

    Growth Hormone (GH) inhibits insulin action, and high level GH can result insulin resistance and diabetes in both mice and humans. GH receptor (GHR) gene disrupted mice (GHR-/- mice) have been genetically modified to lack the GHR and GH action. GHR-/- mice are extremely insulin sensitive and have …

    ohiolink Repository record

  5. Environmental DNA is an Effective Method to Monitor Species in Various Freshwater Habitats

    Mullen, Kayleigh2021

    … (detection of multiple species in one reaction) via quantitative Polymerase Chain Reaction. Results from this study, overall, showed that eDNA methods can produce results that reliably reflect target species’ presence across a large catchment area in an efficient manner.</p>

    central-wash Repository record

  6. Examination of gene deletion as a mechanism of RBM5 downregulation in tobacco smoke-associated lung cancer

    Hunt, Sarah C.Laurentian University of Sudbury2017

    … tissue, RBM5 gene copy number was quantified by quantitative polymerase chain reaction. Although deletions were detected in the lung specimens, RBM5 gene copy number was not significantly decreased in lung tumours. RBM5 deletions were detected in the non-tumour DNA of smokers, but not in the …

    laurentian Repository record

  7. Ethylene response and phytohormone-mediated regulation of gene expression in Komagataeibacter xylinus ATCC 53582

    Augimeri, Richard VincentUniversity of Ontario Institute of Technology2016

    … using pellicle assays and reverse transcription quantitative polymerase chain reaction (RT-qPCR), respectively. Ethylene enhanced cellulose yield by upregulating bcsA and bcsB expression, while IAA decreased cellulose yield by downregulating bcsA. Differential gene expression within the bacterial …

    uoit Repository record

  8. Arousal patterns, fungal loads, and wing damage in a surviving population of little brown bats (Myotis lucifugus)

    Daly, Brooke A.2024

    … load (<em>n</em> = 56) was evaluated through quantitative polymerase chain reaction (qPCR). High autumn temperatures (>10°C) favored growth of the fungus, while mid-hibernation temperature (3.2°C) was unfavorable. The mean torpor bout length (TBL) was similar to bats uninfected by WNS (16.8 …

    emich Repository record

  9. An experimental investigation into the mechanisms of bacterial evolution

    Wan, ZhenmaoThe Graduate School and University Center of The City University of New York2001

    … I will develop a growth assay utilizing the quantitative polymerase chain reaction (qPCR) to provide real-time enumeration of genetic marker abundance within bacterial populations. Second, I will focus on horizontal gene transfer in <em>E. coli</em> occurring through a process called …

    cuny-grad Repository record

  10. Diversity of the marine cyanobacterium Trichodesmium : characterization of the Woods Hole culture collection and quantification of field populations

    Hynes, Annette MichelleMassachusetts Institute of Technology2009

    … clade had similar pigmentation. I developed a quantitative polymerase chain reaction (qPCR) method to distinguish between these two clades. In field populations of the Atlantic and Pacific Oceans, the qPCR method revealed that clade II Trichodesmium spp. were more prominent than clade I in the …

    mit Repository record

  11. Influence of Petroleum Deposit Geometry on Local Gradient of Electron Acceptors and Microbial Catabolic Potential

    Singh, GargiVirginia Tech2012

    … electron acceptor and catabolic conditions via quantitative polymerase chain reaction of dsrA (sulfate-reduction), mcrA (methanogenesis), and cat23 (oxygenation of aromatics) genes in column cores suggested more extensive anaerobic conditions induced by the sheet relative to the ball geometry. …

    vt Repository record

  12. Aspects on local recurrence after rectal cancer surgery

    Kodeda, Karl2012

    … with array-comparative genomic hybridisation and quantitative polymerase chain reaction. Results: The majority of studied patients were symptomatic when diagnosed with local recurrence, deemed incurable, not well palliated and had a poor outcome. Aspects on the surgical management could partly …

    goteborg Repository record

  13. Variations of Vibrio Populations in Pacific White Shrimp, <i>Litopenaeus vannamei</i>, Aquaculture Raceways

    Berutti, Tracy Rene2018

    … yields. This study performed real-time qPCR (quantitative polymerase chain reaction) using primers targeting 16s rDNA sequences for total bacteria, the <em>Vibrio</em> genus, and <em>Vibrio parahaemolyticus</em> in aquaculture tanks over the growing season. Total DNA concentrations in each …

    usm Repository record

  14. Repair of DNA-protein crosslinks in mammalian cells

    Chesner, Lisa2018

    … assay called strand-specific primer extension-quantitative polymerase chain reaction (SSPE-qPCR) used to study the repair of DNA-protein crosslinks in mammalian cells. DNA-protein crosslinks (DPCs) are bulky lesions which disrupt important cell processes such as transcription and replication. …

    umn Repository record

  15. Persistence of Bacteroides ovatus under simulated sunlight irradiation

    Dong, ShengkunUniversity of Illinois at Urbana-Champaign2014

    … propidium monoazide (PMA) treatment and quantitative polymerase chain reaction (qPCR) detection, the decay rates of B. ovatus were determined in the presence of exogenous photosensitizers and salinity up to 39.5 parts per thousand at 27oC. UVB was found to be important for B.ovatus decay, …

    uiuc Repository record

  16. Effect of Soil Type, Composting, and Antibiotic Use on Fate of Antibiotic Resistance Genes and Microbial Community Composition in Dairy and Beef Manure Applied Soils

    Pankow, Christine AnnVirginia Tech2017

    … through novel metagenomic techniques and quantitative polymerase chain reaction of sul1, tet(W), and 16S rRNA gene, while the microbial community composition was determined via 16S rRNA amplicon sequencing. The results indicated that while prior antibiotic administration elevated the …

    vt Repository record

  17. Effect of wastewater colloids on membrane removal of microconstituent antibiotic resistance genes

    Riquelme Breazeal, Maria VirginiaVirginia Tech2011

    … (β-lactam resistance) ARGs were tracked by quantitative polymerase chain reaction through a cascade of membrane filtration steps. To gain insight into potential associations occurring between ARGs and colloidal material, the wastewater colloids were characterized by scanning electron …

    vt Repository record

  18. Biodegradation of QACs in lake and river water and effects on the microbial community

    Joseph, Frantz2025

    … and generally increased with repeated amendment. Quantitative polymerase chain reaction (qPCR) results showed a higher abundance of several ARGs (qacF, mexB, and sul1) in Mississippi River water than in Trout Lake samples. ARGs also tended to increase in abundance with repeated QAC amendments and …

    umn Repository record

  19. Managing Phomopsis Stem Canker of Sunflower Using Improved Diagnosis and Quantification of the Causal Pathogens

    Olson, Taylor Rae2017

    … of the disease with effective diagnostics, two quantitative polymerase chain reaction (qPCR) assays were designed from the translation elongation factor region (EF1-α) of <em>D. helianthi</em> and <em>D. gulyae</em>. The specificity of the two qPCR assays was validated by examining the assays …

    sdstate Repository record

  20. CaV3.2 Channels and BKCa-Mediated Feedback in Vascular Smooth Muscle

    Hashad, Ahmed MohamedCumming School of Medicine2018

    … pressure myography, immunohistochemistry, quantitative polymerase chain reaction (qPCR) and computational modeling. In initial work, perforated patch electrophysiology was used in concert with Ca2+ imaging to illustrate the coordinated interplay between CaV3.2 and two other Ca2+ permeable …

    calgary Repository record

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