Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 196 for “"Quantitative PCR"”.
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Development and Validation of Quantitative PCR Assays for DNA-Based Newborn Screening of 22q11.2 Deletion Syndrome, Spinal Muscular Atrophy, Severe Combined Immunodeficiency and Congenital Cytomegalovirus Infection
… on the OpenArray system and in multiplexed qPCR reactions. SNP detection of homozygous SMN1 deletions in SMA, CNV detection in the 22q11.2 critical region, and quantification of the SCID biomarker, T-cell receptor excision circles (TRECs) and CMV were all required for disease confirmation. …
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Role of the Intron 13 Polypyrimidine Tract in Soluble Flt-1 Expression
… and sFlt-1 protein and mRNA were compared using quantitative PCR, RNA hybridization analysis, and protein immunoblotting. Results from quantitative PCR showed that purine substitutions were associated with 120 to 350 fold decreases in Flt-1 mRNA (normalized against neor), consistent with less …
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Effects of Heterodera Glycines Population Densities on Fusarium Solani F. Sp. Glycines Colonization of Soybean Roots and Development of Sudden Death Syndrome
… explored, relative quantification with real-time quantitative PCR (QPCR), to estimate fungal colonization of soybean roots. QPCR was more sensitive than the plating bioassays at detecting the fungus: Fsg DNA was detected in 371 out of 372 root samples with QPCR, while CFU were only detected in …
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Regional whole plant and molecular response of Kochia scoparia to glyphosate
… and assess the level of resistance. Then PCR, quantitative PCR, sequencing, and immunoblotting techniques were used to determine the mechanism responsible for glyphosate resistance. Sequence of the EPSPS binding site proline confirmed that amino acid substitution at that residue was not …
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Molecular Characterization of Early Dedifferentiation in Newt Forelimb Regeneration
… throughout limb and tail regeneration. We used quantitative PCR (qPCR) to validate the expression of a subset of these genes [β-catenin, wntless, dapper, thymosin-β 4 (Tβ4), and thymosin-β 10/15 (Tβ10/15)] in regenerating limb and tail tissue, as well as in differentiating newt myoblasts. We …
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TOWARDS AN UNDERSTANDING OF GENETIC SELECTION IN BREAST CANCER
… regions. Stable DNA targets were confirmed by quantitative PCR in six breast cancer cell lines. Stable regions contained 9,463 protein coding genes, of which expression of 7,489 were analyzed. We found 78% (5804/7489) of genes had both stable copy number and expression. The breast cancer genome …
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Aquaporin 3 Water Channel Protein Gene Expression in Squalis Acanthias Dogfish Shark
… has 73.8% amino acid identity to human AQP3. Quantitative PCR experiments suggest that constitutive AQP3 expression in the esophagus and kidney may be regulated by external salinity, but the role of AQP3 in water homeostasis of marine elasmobranchs such as Squalus acanthias is yet to be …
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Investigating the epidemiology of Terrapene herpesvirus 1 in freeranging eastern box turtle populations
… populations are less defined. In this thesis, a quantitative PCR was developed that detected a 58 base pair segment of the DNA polymerase gene segment of Terrapene herpesvirus 1 (Order: Herpesvirales; Family: Herpesviridae; Subfamily: Alphaherpesvirinae; Genus: Scutavirus). This assay was used to …
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The role of the cytochrome B and cytochrome C oxidase III genes in the immune response of the South African abalone, Haliotis midae
… study sought to confirm these results by semi-quantitative PCR and to further elucidate the roles of these genes, and thus the electron transport system, in the abalone immune response.
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Geobiology of marine magnetotactic bacteria
… marine basin, was used as a model system for quantitative community studies. Magnetiteproducing MTB predominate slightly above the oxic-anoxic interface and greigiteproducing MTB predominate in sulfidic waters. A quantitative PCR (QPCR) assay was developed and applied to enumerate four major …
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Exploring Novel Immunodiagnostics for Prion Disease
… isoform-specific antibodies. Here, we describe a quantitative PCR method to explore prion protein epitope variability and availability by leveraging proximity ligation technology (PLA). We use a repertoire of nine known monoclonal anti-PrP antibodies to assess differences in prion protein …
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Elucidating the Function of Krüppel Homolog 1 (Kr-h1) Associated Proteins (KAPs) in Aedes aegypti Reproduction Through RNA Interference-Mediated Downregulation
… of DP-1, accompanied by evaluations using quantitative PCR (qPCR), Western blotting (WB), co-immunoprecipitation (Co-IP), follicle length measurement, and egg counting to assess the role of DP-1 in reproductive functions. For the first time, the inhibition of DP-1 expression was found to …
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Genomics-Based Characterization of the Microbial Community and Plasmidome of the Regina Wastewater Treatment Facility and the Surrounding Aquatic Environment
… like high-throughput metagenomic sequencing and quantitative PCR to gain more information about the microbial communities in a wastewater treatment environment. This thesis focuses on three aspects of the microbial communities in the Regina WWTP: (1) the composition of the activated sludge …
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Tongue dorsum swab processing for the detection of tuberculosis with Cepheid Xpert® MTB/RIF Ultra
… results have come from studies that used manual quantitative PCR (qPCR) to analyze swabs. Studies using the automated Cepheid Xpert® MTB/RIF Ultra qPCR test (Xpert Ultra) to analyze tongue swabs have yielded more modest results. Using tongue swabs collected from TB-negative volunteers and spiked …
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Microvessel structure formation in a 3D perfused co-culture of rat hepatocytes and liver endothelial cells
… -7% on day 1 to -12% on day 13, as determined by quantitative PCR on genomic DNA. The maintenance and morphogenesis of endothelial cells were not observed in parallel 2D collagen gel sandwich cultures. Immunohistochemistry further confirmed the presence of sinusoidal endothelia within the 3D …
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Host Specific Microbial Source Tracking and Quantification Using a Partial 16SrRNA Sequence of Bacteroides on the Finley River, a Tributary of the James River in Southwest Missouri
… and genotypic strategies. In this study, quantitative PCR was used to evaluate water samples for species specific (cow and human) Bacteroides in the Finley River. The Finley River is susceptible to impacts from both bovine and humans as it flows through farmland and urban areas. Water …
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The Evolution of Sex Determination and the DMRT1 Gene in the Japanese Gecko (Gekko japonicus)
… of the <em>Dmrt1</em> gene was assessed using quantitative PCR (qPCR). I was not able to quantify the use of the <em>Dmrt1</em> gene between males and females. However, my study does not allow me to exclude the possibility that <em>G. japonicus</em> may use a GSD system with a TSD override, …
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Dynamic DNA Nanotechnology for Probing Single Nucleotide Variants and DNA Modifications
… design, and the polymerase chain reaction (PCR) has been built, it still faces practical problems. For example, the natural DNA is usually in double-stranded form whereas most hybridization probes aim for single-stranded targets; the concentration of extracted DNA samples is totally unknown …
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Quantification of multiple waterborne pathogens in drinking water, drainage channels, and surface waters in Kampala, Uganda over seven months of seasonal variation
… and public health. In this study, microfluidic quantitative PCR (MFQPCR) was used to assess the presence of human enteric pathogens in protected springs, a public tap, drainage channels, and surface water in Kampala, Uganda from November 2014 to May 2015. Because waterborne disease incidence in …
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Expression of dsr messenger RNA by sulfate reducing bacteria with varying substrate and temperature
… nucleic acids and measured biomass, then used quantitative PCR to measure the number of transcripts of dsr mRNA per cell. We find a clear relationship between dsr expression and the rate of sulfate reduction per cell. Considering the difficulty involved in determining sulfate reduction rates in …
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