Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"Quadruplex DNA"”.
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Development of nanobodies as tools to investigate G-quadruplex DNA secondary structures
Guanine-rich sequences in DNA can fold into four-stranded alternative secondary structures called G-quadruplexes (G4). G4s are suggested to be involved in key genome functions such as transcription, genome stability and epigenetic regulation, together with numerous connections to cancer biology. …
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Interactions of small molecules with duplex DNA and lesion containing G-quadruplex DNA
… to study their interactions with duplex DNA. The nickel complexes were found to selectively oxidize guanines in bulged DNA structures in the presence of oxidant and notably NiCR-2H oxidizes guanines more efficiently than NiCR. According to 1 H NMR studies, NiCR-2H binds strongly to the N7 …
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Study of DNA damage on DNA G-quadruplexes and biophysical evaluation of the effects of modified bases (lesions) on their conformation and stability
<p>Exposure of DNA to reactive oxygen species (ROS) results in the modified nucleobases (lesions) as well as strand scissions under physiological conditions. Due to its lowest oxidation potential (1.29 eV), guanine is the most easily oxidisable nucleobase. Furthermore, it has been observed that the …
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Synthesis and biophysical evaluation of thiazole orange derivatives as DNA binding ligands
<p>Guanine-rich telomeric DNA at the end of chromosomes can form a unique DNA tertiary structure - G-quadruplex, which is known to inhibit the binding of telomerase to telomeric regions in cancer cells and thus regulate unrestricted cancer cell growth. Hence, G-quadruplex DNA has recently become a …
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Porphyrin Cross-Linkers for Generating Soluble Molecularly Imprinted Polymers from Polyethyleneimine
… for the preparation of soluble MIPs targeting quadruplex DNA, specifically the human telomeric repeat (TTAGGG)<sub>4</sub>. To do so we have synthesized a series of <em>meso</em>-substituted, water soluble, tetracationic pyridinium porphyrins which we have successfully coupled to …
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The synthesis of macrocyclic molecules related to telomestatin
G-quadruplex DNA represents an attractive target for the development of anti-cancer therapeutic agents as it plays important roles in transcriptional regulation and the maintenance of telomeric length. Oxazole-containing macrocycles such as telomestatin are considered to be a promissing new class …
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NOVEL APPROACHES FOR CONTROLLING TARGET SELECTIVITY AND PHARMACOLOGICAL PROPERTIES OF PLATINUM-INTERCALATOR-BASED ANTICANCER AGENTS
Traditional DNA-targeted anticancer agents, such as platinum-based therapies, have been a mainstay in the treatment of aggressive solid malignancies in the clinical setting. Unfortunately, due to multifactorial drug resistance and systemic toxicity the clinical efficacy of these drugs is severely …
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Characterization of inter-genomic and telomeric G-quadruplex structures through atomic force microscopy
The G-quadruplex DNA structure, a secondary folding motif of DNA, is found throughout the genome. The dynamics of these structures has been seen to affect processes such as telomerase elongation and regulation of gene transcription. Typical investigation techniques require these structures to be …
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IMPROVED SYNTHESIS OF 3-ARYL-ISOXAZOLES AS INTERMEDIATES FOR NOVEL G-QUADRUPLEX BINDING ANTI-TUMOR AGENTS
As a promising new target for chemotherapy G-quadruplexes (G4) have drawn great interest from the scientific community. Current chemotherapeutic agents exhibit broad toxicity to patients; G4 has the potential to be selectively targeted by novel chemotherapeutic agents that exhibit toxicity specific …
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IMPROVED SYNTHESIS OF 3-ARYL-ISOXAZOLES AS INTERMEDIATES FOR NOVEL G-QUADRUPLEX BINDING ANTI-TUMOR AGENTS
As a promising new target for chemotherapy G-quadruplexes (G4) have drawn great interest from the scientific community. Current chemotherapeutic agents exhibit broad toxicity to patients; G4 has the potential to be selectively targeted by novel chemotherapeutic agents that exhibit toxicity specific …
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Evaluation of telomerase activity and telomerase inhibitors in Head and Neck cancer
… evaluation of a series of small molecule G–quadruplex DNA binding agents as potential telomerase inhibitors. A total of 19 telomerase inhibitors were identified but of these, only 4 were specific inhibitors of telomerase. These compounds also caused toxicity to cell lines following a 2 hour …
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Characterizing and targeting the genomic consequences of ATRX deficiency in glioma
… glioma. Loss of ATRX gives rise to abnormal G-quadruplex DNA secondary structures at GC-rich sites of the genome, such as telomeric and pericentromeric regions, enhancing replication stress and genomic instability. These mutations are mutually exclusive with TERT promoter mutations, promoting …
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Studies on Genomic G-Quadruplexes
The double-helical structure of DNA is well known, and the basis of modern genetics and molecular biology. However, DNA is polymorphic, and can adopt a variety of different structures. In this dissertation, I focus on a four-stranded structure that can be formed by guanine-rich sequences. These are …
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G-Triple Chemiluminescent DNAZyme Based Biosensor for the Detection of Calcium(II) Ions in Solution and the Biological Functionality of Kinase Enzymes and the Rationale for their Employment as Promising Drug Targets, Substrate Specific Inhibition of P38alpha
G-quadruplex DNA structures, which are formed from planar stacks of G-tetrads and follow Hoogsteen hydrogen bonding, have recently received attention as recognition elements in bioanalytical assays. DNAzymes are functionally active, synthetic single-stranded DNA molecules that are capable of …
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Interaction with biomolecules and biological activity of metal complexes and silver atomic quantum clusters
En este trabajo se exploran los mecanismos a través de los cuales ejercen su actividad biológica compuestos de diversa naturaleza con potencial actividad anticancerígena. Este tipo de estudios constituyen las primeras etapas básicas en la investigación contra el cáncer, ya que tienen como objetivo …
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The effect of replication impediments on differentiation
… endoderm while replication was perturbed using DNA damaging agents, low dose hydroxyurea and G-quadruplex secondary structure stabilisation. DNA damage was induced both in the undifferentiated state and during differentiation. When damage was induced during differentiation, greater levels of …
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Crystallographic studies of interactions between ligands and DNA oligonucleotides
… One are two out of only five crystallographic quadruplex/ligand structures reported to date. In both structures daunomycin molecules stack onto a terminal G quartet preventing the G4 quadruplex from destacking and unwinding. The number of interacting ligand molecules depends on the quadruplex …
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Vulnerability to G - Quadruplexes in BRCA2 - Null Medulloblastoma: A Protective Role for the PIF1 Helicase
… with biallelic mutations in the essential DNA repair factor BRCA2 are highly predisposed to develop the most common pediatric brain cancer medulloblastoma (MB) before three years of age. Medulloblastoma is often the first malignancy in these children who have a high likelihood of developing …