Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 67 for “"QH426 Genetics"”.
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Comparative genomics of nematodes: Caenorhabditis elegans as a tool to study the Haemonchus contortus genome
The genome of the Trichostrongylid nematode parasite of small ruminants Haemonchus contortus is being sequenced at the Pathogen Sequencing Unit of the Wellcome Trust Sanger Institute, Cambridge, UK. Currently, in excess of 800 Mb of genomic sequence is available for this on-going project …
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Genetic studies with Bordetella pertussis
Summary available: p.18
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Manipulation of second messengers by ectopic expression of receptors in transgenic Drosophila
The combination of physiology and genetics affords opportunities to perform experiments with greater precision, and with less risk of artefact, than heretofore possible. This is illustrated here by demonstrating the selective manipulation of the second messengers cyclic GMP (cGMP), cyclic AMP …
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Sequence selectivity of the resolvase catalytic domain: implications for Z-resolvase design
The extent of sequence specificity of the Tn3 resolvase catalytic domain was investigated by creating libraries of Tn3 site I variants in which all of the central 16 bp were systematically randomised in overlapping 4 bp blocks and recombination deficient and recombination proficient site I variants …
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Understanding the pathogenesis of myotonic dystrophy type 1
To identify the full range of targets and the pathogenic consequences, we sought to mimic the pathogenesis of myotonic dystrophy type 1 with temporal and spatial control: temporal to reproduce the developmental pathogenesis of the congenital form, and spatial to isolate tissue specific pathology. …
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In vivo studies of repressors of RNA polymerase III transcription
RNA polymerase III (Pol III) is responsible for transcribing a relatively small but vital set of genes, including 5S rRNA and tRNAs. Pol III transcription has been shown to be upregulated in transformed and cancer cells, suggesting an important role in cell growth and proliferation. Its tight …
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Development of targeted gene delivery vectors to assess cardiac overexpresion of ACE2 in vivo
The renin angiotensin system is often maninpulated clinically for the treatment of hypertension and heart failure. This pathway is of major clinical importance and it is thus a major target for therapy. The incidence of cardiovascular diseases continues to increase worldwide, highlighting the need …
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Functional genomics in the stroke-prone spontaneously hypertensive rat: genome wide and candidate gene analysis
The stroke-prone spontaneously hypertensive rat (SHRSP) is an inbred model of hypertension. Renal microarrays and functional genomic strategies investigated chromosome 2 candidate hypertension genes, focussing on the oxidative-stress defence gene, glutathione s-transferse mu type 1 (Gstm1). …
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Dissecting the binding mechanisms of transcription factors to DNA using a statistical thermodynamics framework.
At the heart of gene regulation are Transcription Factors (TFs), proteins which bind to DNA in a sequence specific manner and drive the activation or repression of genes. Statistical thermodynamics has shown to be a promising avenue to describe the binding mechanisms of TFs. Here, I present …
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Cell Signalling and MicroRNAs: Regulation and Evolution
Cell signalling is among the most studied topics in modern biology, it allows cells to communicate with each other and their environment and thus, orchestrates the entire functioning of organisms. Despite being studied for more than a century, the field of cell signalling has recently evolved as …
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Considering the impact of smoking on DNA methylation in Understanding Society
Smoking is a huge issue for social health and consequently there has been much research considering the relationship between tobacco use and many biological processes. One interesting field of study has identified epigenetic signatures of smoking using DNA methylation profiles. Up to this point, …
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Identification and analysis of Arabidopsis thiamine pyrophosphate transporters
Thiamine pyrophosphate (TPP) serves as a cofactor in universal metabolic pathways, including glycolysis, the pentose phosphate pathway (PPP) and the tricarboxylic acid cycle; moreover, it is essential for the proper functioning of all organisms. Recently, several steps of the plant thiamine …
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Identifying the molecular components that matter: a statistical modelling approach to linking functional genomics data to cell physiology
Functional genomics technologies, in which thousands of mRNAs, proteins, or metabolites can be measured in single experiments, have contributed to reshape biological investigations. One of the most important issues in the analysis of the generated large datasets is the selection of relatively small …
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Causes and consequences of inbreeding in the banded mongoose
Inbreeding and inbreeding depression have been studied since the days of Darwin and yet we are still making important discoveries today. These discoveries can inform conservation practices as inbreeding depression can contribute to population extinction. Inbreeding is also an important subject in …
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Biochemical and structural studies of histone and associated proteins from chick and human nuclei
Chromatin is the complex of DNA, histones and non-histone proteins that make up chromosomes and the region of dispersed chromosomes during the interphases and S phase of the cell cycle. It is found inside cell nuclei in eukaryotic cells. There are many important proteins in the nuclei which are …
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Linkage mapping and genetic analysis of Trypanosoma brucei
Trypanosoma brucei is a protozoan parasite of major public health and economic importance in sub-Saharan Africa, where it is the causative agent of sleeping sickness in man and Nagana in cattle. The complete genome sequence of T.brucei is now available and the diploid genetic system has recently …
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Implementation, adaptation and evaluation of statistical analysis techniques for next generation sequencing data
Deep sequencing is a new high‐throughput sequencing technology intended to lower the cost of DNA sequencing further than what was previously thought possible using standard methods. Analysis of sequencing data such as SAGE (serial analysis of gene expression) and microarray data has been a popular …
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Drosophila as a model for the Anopheles Malpighian tubule
The insect Malpighian tubule is involved in osmoregulation, detoxification and immune function, physiological processes which are essential for insect development and survival. As the Malpighian tubules contain many ion channels and transporters, they could be an effective tissue for targeting with …
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Iron-dependent regulation of gene expression in Corynebacterium pseudotuberculosis
This study set out to analyse C. pseudotuberculosis within an environment relevant to that which would be encountered within its natural host. The impact of the availability of iron within the growth environment of numerous bacteria has been widely reported, and an equivalent investigation was …
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The role of the chromatin protein HMGN3 in cytokine induced gene expression
HMGN proteins modulate chromatin structure by binding to chromatin, unfolding nucleosome arrays and altering the pattern of histone modifications. HMGN3 is unique amongst the HMGN family as two splice variants are produced from the HMGN3 transcript. HMGN3 was shown to regulate TNFα-induced …
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