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Showing 1 to 16 of 16 for “"Pyruvate oxidase"”.
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Pyruvate Oxidase: Mechanism of Lipid Activation
Made available in DSpace on 2014-12-14T04:27:40Z (GMT). No. of bitstreams: 1 7803940.pdf: 8298143 bytes, checksum: 073b2078a6d81cca2e575b541f2d2262 (MD5) Previous issue date: 1977
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Ligand-Binding Properties of Escherichia Coli Pyruvate Oxidase
Made available in DSpace on 2014-12-13T20:10:33Z (GMT). No. of bitstreams: 1 7913567.pdf: 6615798 bytes, checksum: e50e91608fa1b94872aa9b0ce11516a0 (MD5) Previous issue date: 1978
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Further Studies on Pyruvate-Oxidase of Escherichia Coli
Made available in DSpace on 2014-12-10T23:24:29Z (GMT). No. of bitstreams: 1 7317365.pdf: 5052932 bytes, checksum: 5a7a3040a6ad64e91771a4c21382558b (MD5) Previous issue date: 1972
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Circular dichroism studies of Escherichia coli pyruvate oxidase and secondary structure and lipid binding properties of the alpha peptide from pyruvate oxidase
Pyruvate oxidase, a tetrameric enzyme consisting of four identicle subunits, undergoes a change in conformation with the binding of TPP and pyruvate. Circular dichroism measurements show the change in secondary structure with this ligand induced conformational change. Circular dichroism spectra …
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Studies on the Lipid-Protein Interactions of Pyruvate Oxidase
Made available in DSpace on 2014-12-13T20:10:35Z (GMT). No. of bitstreams: 1 7913605.pdf: 4194774 bytes, checksum: 833d642fed06417e585b36180ccb9408 (MD5) Previous issue date: 1978
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Studies on the Pyruvate Oxidase System of Escherichia Coli
Made available in DSpace on 2014-12-09T17:36:05Z (GMT). No. of bitstreams: 1 6901326.pdf: 4553325 bytes, checksum: 8e10e025f68eedbe3e69c5b0cfa404c4 (MD5) Previous issue date: 1968
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Pyruvate Oxidase and the Cytochrome D Terminal Oxidase of Escherichia Coli
… aerobic electron transport system were studied, pyruvate oxidase and the cytochrome d terminal oxidase. Three general subjects were treated: (i) the active site structure and catalytic mechanism of pyruvate oxidase, (ii) the role of pyruvate oxidase in the E. coli aerobic electron transport …
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Studies on the Proteolytic Activation of the Membrane Enzyme, Pyruvate Oxidase
Pyruvate oxidase is a peripheral membrane flavoenzyme which has been purified to homogeneity from Escherichia coli. The enzyme is a tetramer of four apparently identical subunits with a molecular weight of 60,000 each. Amino acid analysis reveals that only 46% of the amino acid residues are …
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Genetic and Biochemical Characterization of the Lipid-Protein Interactions of Pyruvate Oxidase
The pyruvate oxidase structural gene (poxB) of Escherichia coli was cloned into derivatives of plasmid pBR322. The gene was first cloned into a cosmid vector by selection for the tetracycline resistance determinant of a closely linked Tn 10 insertion since no direct selection was available. …
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Studies on the Flavin Binding Properties of Native and Protease-Activated Pyruvate Oxidase From Escherichia Coli
Pyruvate oxidase, a tetrameric enzyme consisting of four identical subunits, dissociates into apoenzyme monomers and free FAD when treated with acid ammonium sulfate in the presence of high concentrations of potassium bromide. Reconstitution of the native enzymatically-active protein can be …
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Studies on the Mechanism of the Pyruvate Oxidase Flavoprotein of Escherichia Coli (Rapid Kinetics, Biochemical Software, Lipid-Activated Enzyme)
The pyruvate oxidase flavoprotein catalyzes the oxidative decarboxylation of pyruvate to acetate and carbon dioxide. Flavin adenine dinucleotide and thiamin pyrophosphate are cofactors. The enzymatic activity of the purfied flavoprotein is very low in the absence of lipids. Following reduction by …
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Investigations into the biosynthesis and mode of action of the phosphonate antibiotic dehydrophos
… acetylphosphonate, a structural analogue of pyruvate, and known inhibitor of pyruvate dehydrogenase and pyruvate oxidase. To identify genes necessary for the biosynthesis of this unusual compound we screened a fosmid library of S. luridus for the presence of the phosphoenolpyruvate mutase …
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Development of Electrochemical Biosensors for Potential Liver Disease Detections of ALT & AST and Application of Ionic Liquid into Biosensing-Modified Electrodes
… blood. A glutamate biosensor with glutamate oxidase (GluOx) immobilization was desirable to the quantifications of both aminotransferase activities sequentially using a single biosensor design. The kinetic-controlled measurement was undertaken. In this approach, AST was measured accurately …
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Biochemical sensing using Siloxane polymer waveguides
… of continuously monitoring glucose, lactate and pyruvate concentrations in the brain fluid, hence the contribution to the current trend in the advancement of portable lab-on-chip technologies for the deployment of point-of-care diagnostic tools. A novel recognition layer has been developed based …
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Metabolic engineering and modelling of Escherichia coli for the production of succinate
… acetate kinase, phospho-acetyltransferase and pyruvate oxidase were knocked out. This resulted in a significant decrease in acetate production but not in the total elimination. Several alternatives such as citrate lyase and acetate CoA-transferase were evaluated, but without success. The …