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Showing 1 to 20 of 57 for “"Pyruvate kinase."”.
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Identification of Novel Allosteric Regulators of Human Erythrocyte Pyruvate Kinase
Erythrocyte pyruvate kinase (R-PK) is a key glycolytic enzyme catalyzing the transphosphorylation of phosphoenolpyruvate (PEP) and ADP to pyruvate and ATP respectively3,4. The substrate PEP and product pyruvate of this reaction are involved in a number of energetic and biosynthetic pathways; hence …
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The purification and properties of pyruvate kinase from baker's yeast
Thesis (M.S.)--Michigan State University. Dept. of Biochemistry, 1966
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The role of pyruvate kinase regulation in tumor growth and metabolism
… unique anabolic needs of proliferating cells. Pyruvate kinase, the enzyme catalyzing the final step of glycolysis, is an important point of regulation. Mammals have four pyruvate kinase isoforms, and one in particular, the M2 isoform, is preferentially expressed in proliferative tissues, …
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Clinical review and experimental evaluation of tumour M2-pyruvate kinase in pancreatic cancer
… Tumour M2-PK, a dimeric form of M2 isoenzyme of pyruvate kinase, in GI cancer and carried out a meta-analysis of the clinical data on pancreatic cancer. Experimental work evaluated the measurement of M2-pyruvate kinase in human pancreatic cancer cell lines with altered microenvironment (hypoxia, …
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Two isoforms of pyruvate kinase enzymes in Pseudomonas aeruginosa with distinct functional and structural properties
In most organisms, phosphofructokinase (PFK) and pyruvate kinase (PK) are the key glycolytic regulatory enzymes. However, the opportunistic human pathogen, Pseudomonas aeruginosa, relies entirely on the Entner-Doudoroff pathway for glycolysis, and consequently, does not encode a PFK homologue. It …
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Examining the roles of the pyruvate kinase isoforms, PKMI1 and PKM2, in systemic metabolism and tumor development
… product generates the PKM1 and PKM2 isoforms of pyruvate kinase, and PKM2 expression is closely linked to embryogenesis, tissue regeneration, and cancer. PKM1 expression, on the other hand, is restricted mostly to skeletal muscle, heart, and brain. To interrogate the functional requirement for …
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Regulation of pyruvate kinase and glycerol-3-phosphate dehydrogenase in the freeze tolerant goldenrod gall fly: role in polyol cryoprotectant production
… strategy of cold hardiness. This study examines pyruvate kinase (PK) and glycerol-3-phosphate dehydrogenase (G3PDH) that were purified from gall fly larvae acclimated to 5oC (control) and −15oC (freeze-exposed). The roles that these enzymes play in polyol cryoprotectant synthesis and metabolic …
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Physiological studies of bitter pit in apple
… from the field study, the relationship between pyruvate kinase activity, fruit cation concentration and bitter pit was investigated. Pyruvate kinase activity during early fruit growth was higher in fruit which developed 100% bitter pit after storage (MgCl, spray + bag), than in fruit that …
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The Performance of Circulating Biomarkers in the Prediction of Response to Neoadjuvant Therapy in Patients with Oesophago-gastric Cancer
… surgery. Levels of circulating biomarkers M2-Pyruvate kinase, alkaline phosphatase, CA19-9, CEA and CA 72-4 were measured in patients before and after administering the first cycle of chemotherapy. Binary logistic regression analysis was performed to assess the ability of biomarkers to predict …
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Defining the contributors to mammalian cell mass
… properties of two glycolytic enzymes, pyruvate kinase and enolase. Although pyruvate kinase isoform M2 (PKM2) expression enables proliferation in some contexts, we demonstrate that this is not because of its putative activity as a protein kinase. We additionally characterize a novel …
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Characterization of Mechanisms That Mediate Cancer Metastatic Colonization
… for liver colonization. Liver and red blood cell pyruvate kinase (PKLR) was identified as a driver of liver metastasis in experimental models. In patients, PKLR was found to be expressed at higher levels in liver metastases relative to primary colorectal cancer tumors and also overexpressed in the …
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Exploring Beta Cell NADPH production using Organelle-targeted Apollo-NADP+ Sensors
… Senescence impacts mitochondrial-dependent pyruvate cycling and nicotinamide nucleotide transhydrogenase (NNT) activity, which are important for production of beta cell NADPH to support biomolecule synthesis during cell growth and for the quenching of reactive oxygen species generated during …
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We are what we eat : identifying a regulatory crosstalk between central carbon metabolism and cell division in bacteria
… wherein disrupting glycolysis by deleting the pyruvate kinase (𝘱𝘺𝘬) enzyme was shown to affect the correct positioning of the Z ring at the cell centre, such that 1/3ʳᵈ of the Z rings formed at acentral positions. Interestingly, the addition of pyruvate (the end product of the pyruvate kinase …
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Examining cancer metabolism in vivo
… to test the requirements for isoform-specific pyruvate kinase expression. We applied these methods to three mouse models of cancer (models of human lung, pancreas, and prostate cancer) to better characterize metabolism and identify potential metabolic vulnerabilities in these tumors. Our …
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Synthesis and Biological Evaluation of 2'-Deoxyribonucleotide Analogs
… found to be a substrate for phosphorylation by pyruvate kinase, and the triphosphate was found to be an acceptable replacement for 2'-deoxyadenosine-5'-triphosphate in DNA nick translation by E. coli DNA polymerase I.
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Assessing the Effects of Temperature, Feeding Status, and the Microbiome on the Maximal Activities of Several Enzymes Important to Energy Production and Ammonia Detoxification in the Intestines of Teleost Fish
… of Na+, K+-ATPase (NKA), citrate synthase, pyruvate kinase, and glutamine synthetase (GS). Increased NKA activity following a meal suggested an increase in ATP demand, and amplified GS activity likely assists in enhanced NH3-detoxification processes in the intestinal tract. There was a …
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Comparing Solution-Phase and Gas-Phase Protein Stability Using Ion Mobility and Differential Mobility Mass Spectrometry
… stability using a homo-tetrameric model protein, pyruvate kinase, and four of its point mutants. Efforts to employ a second ion mobility technique differential mobility spectrometry, to determine gas phase stability will also be discussed.
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Studies on mammalian pre-mRNA splicing : connections to transcription and cancer
… and is critical for cancer cell growth. The pyruvate kinase isoform expressed by the cell is a key determinant of glucose usage. Pyruvate kinase in most tissues is produced from the PKM gene, which is alternatively spliced to produce to produce the PKM1 or PKM2 isoforms, which contain exons 9 …
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