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Showing 1 to 3 of 3 for “"Pyrrolysyl-tRNA Synthetase"”.
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STUDIES OF THE PYRROLYSYL-TRNA SYNTHETASE
… the 22nd amino acid encoded by the UAG codon. Pyrrolysyl-tRNA synthetase (PylS) is the specific enzyme that can attach Pyl to its cognate tRNA, the amber suppressor tRNAPyl. The biosynthesis of pyrrolysine required the function of three genes, pylC, pylB, pylD, which were identified in the same …
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Chemical Tools for Exploring IFITM3 S-Palmitoylation and Mechanism
… in mammalian cells in Chapter 3. Using the pyrrolysyl-tRNA synthetase (PylRS)/Pyl tRNACUA system, I showed that unnatural amino acid can be site-specifically incorporated throughout IFITM3 in mammalian cells. The site-specific labeling of IFITM3 with unnatural amino acids has provided new …
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Genetic Code Expansion in Mammalian Cells
… machinery often importing an aminoacyl-tRNA synthetase (aaRS)/tRNA pair that does not cross-react with the endogenous aaRS/tRNA pairs. This approach has allowed for co-translational incorporation of a variety of non-canonical amino acids, including amino acids for photocrosslinking, …