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Showing 1 to 18 of 18 for “"Pyrophosphatase"”.
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A Functional Genomics Approach to the Plant Soluble Pyrophosphatase Family
Although the activities of soluble pyrophosphatases were shown to be essential for a number of prokaryotes and eukaryotes, plants were assumed to lack cytoplasmic soluble pyrophosphatase activity and vacuolar membrane bound proton-pumping pyrophosphatase was accepted as the only enzyme responsible …
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The Role of the C-terminus in Bacillus subtilis inorganic pyrophosphatase activity
Inorganic pyrophosphatases hydrolyse inorganic pyrophosphate ensuring that the pool of phosphate is continuously available in a cell for subsequent metabolic reactions. Bacillus subtilis PPase is the first member of the Family II of PPases, unique not only in terms of physical/biochemical …
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Impact of Genetic Variants in Inosine Triphosphate Pyrophosphatase and Interferon-λ4 on Natural History, Treatment Response and Ribavirin Pharmacology in Hepatitis C Virus Infection
… host genetic variations in inosine triphosphate pyrophosphatase (ITPA) and interferon-λ4 (IFNL4) in relation to cure rates in patient treated with interferon-α and ribavirin as well as ribavirin pharmacology in the setting of chronic HCV infection, and spontaneous resolution of acute HCV …
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Developing New Chemotherapeutic Agents Against Bone Resorption and Parasitic Diseases Through Computer -Aided Drug Design
… as Trypanosoma brucei FPPS, soluble vacuolar pyrophosphatase and phosphoglycerate kinase, all important for the proliferation of Trypanosoma brucei, the causative agent of African sleeping sickness. These predictive models aid in optimizing existing inhibitors and ultimately designing novel …
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Mechanisms of proton translocation in Methanosarcina mazei Gö1
… sowie eine protonentranslozierende Pyrophosphatase.Es konnte gezeigt werden, dass 2-Hydroxyphenazin den Elektronentransport zwischen den Schlüsselenzymen der H2:Heterodisulfid-Oxidoreduktase und der F420H2:Heterodisulfid Oxidoreduktase vermitteln konnte. Dieser Elektronenüberträger …
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Identification of a mitogen-activated protein kinase, p56, which mediates the self-incompatibility response in Papaver rhoeas L.
… phosphorylation of p26, an inorganic pyrophosphatase required for pollen tube growth, depolymerisation of the pollen actin cytoskeleton and ultimately programmed cell-death (PCD). To further understand the mechanisms involved in Papaver rhoeas SI it is important to identify and …
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Studies of the NAD metabolism of Haemophilus influenzae
… precursors. An externally directed nucleotide pyrophosphatase was extracted from the organism and purified 700-fold using ammonium sulfate precipitation, ion-exchange and affinity chromatography. The enzyme was determined to be a periplasmic glycoprotein consisting of a single polypeptide of …
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Nucleotide pirofosfatasi/fosfodiesterasi dal lattice di Euphorbia characias e dai frutti di Opuntia ficus indica: purificazione e caratterizzazione
Thesis reports on two metallo-proteins nucleotide pyrophosphatase/phosphodiesterase purified from Euphorbia characias latex (ELNPP) and from prickly pear (Opuntia ficus indica) fruits (ONPP). ELNPP has a molecular mass of 80 kDa and is formed by two apparently identical subunits, each containing 1 …
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Studies on pyridine nucleotide-dependent processes in Haemophilus influenzae
… Haemophilus influenzae periplasmic nucleotide pyrophosphatase. A cytosolic lactate dehydrogenase, specific for D(-)-lactate was purified to electrophoretic homogeneity 2100-fold with a 14% yield. The purified enzyme was demonstrated to be a tetramer of M, = 135,000. It catalyzes essentially the …
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Dynamics Of Biopolymers On Nanomaterials Studied By Quasielastic Neutron Scattering And Mdsimulations
… specific model proteins, β-Casein and Inorganic Pyrophosphatase (IPPase). </p> <p> RNA and nanodiamond (ND) both are suitable for drug-delivery applications in nano-biotechnology. The architecturally flexible RNA with catalytic functionality forms nanocomposites that can treat life-threatening …
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Charakterisierung der initialen Aufnahme des Kofaktors V (NAD) bei Haemophilus influenzae
… Das periplasmatische Protein NadN wurde als Pyrophosphatase beschrieben, die fähig ist, NAD zu NMN zu hydrolysieren. Weiter wurde eine 5´-Nukleotidase-Aktivität nachgewiesen, mit der NadN NMN zu NR dephosphorylieren kann. Die Relevanz von NadN für die NAD-Aufnahme wurde anhand einer …
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Expression and characterisation of metalloproteins from mycobacterium tuberculosis
… The crystal structure of Rv3628, an inorganic pyrophosphatase (Mtb-PPase), was refined to 2.7 A resolution in space group P3221. Inorganic pyrophosphatases (PPases) are ubiquitous metalloenzymes which belong to the phosphatase superfarnily, and play an essential role in biosynthetic reactions …
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Classical And Ab Initio Qm/mm Simulations Of Bacterial Enzymes
… al.)</p> <p> Chapter 5 discusses the inorganic pyrophosphatase (PPase) mechanism in M. tuberculosis which requires information at the electronic level. The QM/MM hybrid method was used in which the reactive moieties were included in the QM subsystem to achieve accuracy at the electronic level …
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The Golgi Complex of the Hepatocyte: Isolation and Partial Enzomological Characterization
… The Golgi fractions also have high thiamine pyrophosphatase activity. The Golgi fraction contains less than 1% of the cytochrome oxidase specific activity of purified mitochondrial fractions, consistent with our failure to observe mitochondrial contaminants morphologically. The acid …
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Klonierung, Charakterisierung und Überexpression von Pyrophosphatasen aus Beta vulgaris L.
… Arbeit wurden membranständige vakuoläre H+-Pyrophosphatasen (V-PPasen) und lösliche Pyrophosphatasen (S-PPasen) der Zuckerrübe untersucht. Nach der vorherrschenden Meinung erfolgt die Akkumulation von Saccharose in den Vakuolen des Speicherparenchyms durch einen Saccharose/H+-Antiporter …
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Manufacturing GMP-grade mRNA vaccine-associated Enzymes: Bioprocess Development and Economics
… I, Vaccinia Capping Enzyme (VCE), Inorganic Pyrophosphatase (iPPase), and 2’-O-methyltransferase (2OMT), commercially in South Africa under GMP conditions. A four-phase techno-economic methodology was employed, integrating bioprocess simulation in SuperPro Designer with capital and operating …