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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 28 for “"Puromycin"”.
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Synthesis of cytidylyl puromycin
Thesis (M.S.)--Michigan State University. Dept. of Biochemistry, 1969
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Klonierung und Charakterisierung der humanen Puromycin-sensitiven Aminopeptidase
… in eine nicht erwartete Richtung. Mit der Puromycin-sensitiven Aminopeptidase war ein Gen aus der Familie der Metallopeptidasen gefunden worden, eine Exopeptidase mit einem Zink-Ion im katalytischen Zentrum. Proteinchemisch schon vor einigen Jahren isoliert, war über die PSA …
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Inhibition of peptide bond formation by nucleotidyl derivatives of puromycin
Thesis (Ph. D.)--Michigan State University. Dept. of Biochemistry, 1971
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Regulation des Na+/Ca2+-Austauschers in kultivierten Podozyten der Maus
… Na+/Ca2+-Austauschers beteiligt zu sein. <br>Die Puromycin-Nephrose ist seit Jahrzehnten Modell für die Minimal-Change-Glomerulonephritis, in deren Verlauf es zunächst zu einer ausgeprägten Schädigung der Podozyten kommt, deren Nukleinsäure- und Proteinstoffwechsel in besonderer Weise gestört …
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The Isolation of Friend Virus Protein by Zonal Centrifugation
… the sucrose was accomplished by ultrafiltration. Puromycin was used to release the nascent viral protein from the polysomes isolated, and the nascant protein was separated from the polysomes by centrifugation and then concentrated using ultrafiltration. Findings. 1480 mg of protein was isolated …
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Expression Analysis and Stem Cell Engineering
… We hypothesized that engineering ES cells with puromycin acetyltransferase gene: PAC) under the control of the Olig2 promoter would allow for selection of Olig2 expressing neural cells with puromycin. Two targeted ES cell lines were generated with PAC inserted into the Olig2 gene. Both lines …
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The Role of Gene Expression Noise In Mammalian Cell Survival
… point, isogenic cells were treated with various Puromycin concentrations. Evolution experiments revealed that noise hurts drug resistance during low drug dosage while facilitating resistance at a high Puromycin concentration. Drug adaptation for the low-noise gene circuit relied on intra-circuit …
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Inhibition of Margination and Diapedesis of Neutrophils by Protein Synthesis Blockade
… protein synthesis inhibitors, a combination of puromycin and anisomycin. Our strategy, if fine-tuned, could form the basis of a new clinical strategy for the prevention of ARDS.
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Development and characterization of cell-free amino acid incorporation systems from <u>Cellvibrio gilvus</u>
… was greatly inhibited by RBAase and puromycin, moderately inhibited by chloramphenicol, but not effected significantly by DNAase and actinomycin D. The optimum pH and temperature for ¹⁴C-leucine incorporation were 7.8 and 300° C, respectively. Polyamiaes (putrescine, spermine, and …
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In vitro studies on the SecA involvement in the assembly of an integral membrane protein with large hydrophilic domain
… ribosome was released from the polypeptide by puromycin. <br>We could show in this thesis that SecA promotes a partial translocation of nascent Momp2 chains even if they are still attached to the ribosome. This partial translocation became manifest after treatment of the membrane-bound nascent …
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Mechanism of Translational Control by the Fragile X Mental Retardation Protein and Creation of the FMRP CTAG Mouse
… increased ribosome runoff after treatment with puromycin, a drug that acts specifically on translocating ribosomes. In addition to genetic loss-of-function models, FMRPdependent relief of ribosome stalling could be induced by acute biochemical removal of FMRP from polysomes, indicating …
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Rapid Alterations in Rat Brain Tryptophan Hydroxylase Activity; Effects of Adrenal Steroids, Drugs and Stressors
… failure of a second protein synthesis inhibitor, puromycin, from producing the same results found with cycloheximide. Evidence was found to suggest that the availability of reduced cofactors may be the controlling factor in regulating rapid changes in TP-hydroxylase activity.</p>
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Studies on the pathogenesis of coeliac disease
… of the LMI test with gluten are unaffected by puromycin and results of the test performed with purified polymorphs are similar to and correlate with results of the standard test. Added T lymphocytes have no effect. These results contrast with those seen with PPD as antigen. Normal leucocytes …
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The study of wheat striate mosaic virus (WSMV) in wheat callus tissue culture
… protein inhibitors (cycloheximide, 100 μg/ml and puromycin, 200 μg/ml), viral RNA and protein synthesis were studied. Assuming that there are no changes in specific activities of the intracellular RNA and protein precursor pools, or in the amount of RNA and protein synthesis in callus cells, …
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Sequenzierung und molekulare Charakterisierung einer Lon-homologen Protease aus Bartonella henselae und heterologe Expression in Escherichia coli
… werden. Die Anwesenheit von 80 Mikrogramm/ml Puromycin führte dagegen zur drastischen Abnahme der Genexpression. <br>Die Lon-Protease von B. henselae wurde durch heterologe Expression als Fusionsprotein an ein Maltosebindeprotein in E. coli überexprimiert. Durch Aufreinigung über …
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Axonal Translation and Links to Neuropathies
… protein synthesis measured by metabolic and puromycin labelling indicates axons experience stage-dependent alterations in translation rate upon Netrin-1 stimulation. Remarkably, Netrin-1 triggers a burst of β-actin synthesis starting within 20 seconds of cue application at multiple …
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Characterization of Phagosome-Lysosome Fusion in Macrophages
… PMA. The effect of PMA was blocked by 10 μg/ml puromycin or 5 μg/ml cycloheximide when cells were preincubated for 2 hr with the protein synthesis inhibitor and exposure to the inhibitor maintained during incubation with PMA and subsequent assay. Similar treatments with puromycin or …
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Positioning Microtissues on the Differentiation Continuum Reveals their Utility for Studying Skeletal Myopathies and Metabolic Disease
… demonstrating improved accuracy over puromycin-based methods. We uncovered that the myofibrillar protein fraction of microtissues is more stable than the sarcoplasmic fraction, a property of native human muscle absent in 2D myotubes. Finally, to evaluate the relevance of microtissues …
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Investigation Of Posttranscriptional Regulation After Global Brain Ischemia And Reperfusion Injury
… neurons, mRNA granules can be induced with puromycin, which promotes early translation termination and dissociation of ribosomes from mRNA. In hippocampal neurons after 10 minutes global brain ischemia and 8 hours reperfusion, HuR remained constant in nuclear and cytoplasmic fractions and …
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Developing High Purity Embryonic Stem Cell-Derived V2a Interneurons for In Vitro Investigation and Transplantation Following Spinal Cord Injury
… (ESC-V2as) by treating differentiated cells with puromycin. ESC-V2a cultures remained free of proliferative cells, matured into normal glutamatergic neurons, and were capable of forming synapses onto motor neurons in adherent monolayer culture. For further in vitro investigation and …
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