Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"Purine nucleotides"”.
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An in vitro system for studying the mechanism of action of thyroid hormones
… in the presence of IMP as a precursor of purine nucleotides and necessary substrates, stimulated orotic acid-6-¹⁴C incorporation into nuclear RNA. The stimulatory effect was observed with 10⁻⁹ M triiodo-L-thyronine (L-T₃ )and with 10⁻⁵ M thyroxine. Thyroid hormones had no effect on the …
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Studies on the mechanism of action of thyroid hormones
… monophosphate (IMP) is a precursor of the two purine nucleotides, adenosine monophosphate (AMP) and guanosine monophosphate (GMP)e through DEAE-Scphacex, and the reaction IMP~ XMP is catalyzed by: IMP dehydrogenase which was purified in a manner similar to adenylosuccinate synthetase. …
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Factors Influencing Muscle Purine Nucleotide Metabolism.
… the factors influencing the metabolism of purine nucleotides in human skeletal muscle, plasma and urine. Study one investigated the influence of the number of intermittent sprint bouts and the subsequent accumulation of plasma purines and urinary purine excretion. Study two investigated the …
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Identification and Characterization of Drug Targets in the Pyrimidine and Purine Pathways of Trypanosoma brucei
… drug targets in the parasite pyrimidine and purine pathways. The pyrimidine de novo biosynthetic pathway has been well characterized, however little work had been done to evaluate the importance of pyrimidine salvage enzymes. Specifically, my research validates the essentiality two seemingly …
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Structure and regulatory mechanism of human uncoupling protein 1
… properties of UCP1 are regulated by cytosolic purine nucleotides, which inhibit proton conductance and therefore enable activation only in response to cold environments. This thesis aimed to determine the first high-resolution structure of UCP1 to address several unanswered questions about its …
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Platelet Function and Energy Metabolism During Storage in a Well-Defined Experimental Medium
… synthetic medium and correlates this data with purine nucleotide levels determined by high-performance liquid chromatography (HPLC) and other in vitro measures of platelet quality. Platelets prepared from pooled ABO- and Rh- identical platelet-rich plasma were stored as concentrates in the …
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Metabolic regulation of mammalian cell growth and proliferation
… cofactor NAD+, and that decreased production of purine nucleotides downstream of serine limits proliferation. These findings demonstrate that regeneration of NAD+ can be a limitation for serine and nucleotide synthesis that constrains proliferation. We next determined how cells respond to …
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The metabolic responses of high intensity intermittent exercise in healthy untrained adults
… well as significantly greater plasma and urine purine nucleotides post HIIE compared to CON, potentially resulting in greater energy loss. This thesis aims to measure metabolic profiles for a range of HIIE models to understand the mechanism and determine which may be best at inducing an energy …
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Some in vivo effects of triiodothyronine on purine nucleotide synthesis
… of triiodothyronine on the synthesis of hepatic purine nucleotides was investigated in sulfaguanidine-fed hypothyroid rats. To prevent the rapid destruction of nucleotides caused by anoxia and ischemia an apparatus was designed to maintain ether anesthesia while providing normal levels of oxygen. …
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Interrogation of the human glutamate dehydrogenase antenna to elucidate its role in allosteric regulation and disease
… The chimeric GDH lost allosteric regulation by purine nucleotides. Therefore, the purpose of the antenna was deemed to likely facilitate allosteric regulation. The current antenna-less construct, which does not include bacterial sequence, has unexpected and exciting properties as allosteric …
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Analysis of dirhenium carboxylate : purine dinucleotide adducts
… of cancel cells by coordinating to the purine nucleobases in DNA. To investigate the interaction between dimetal compounds and DNA, dirhenium complexes coordinated to purine dinucleotides were isolated and analyzed. LC/MS, HPLC, <sup>1</sup>H NMR, and UV-Visible spectroscopy were used to …
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Reactivity of Re₂(CH₃COO)₂Cl₄·2H₂O with purine DNA dinucleotides
… of dinuclear metal carboxylate compounds to purine DNA nucleobases has been shown to be a source of anticancer activity, and has resulted in intense research to understand the coordination of metal complexes to DNA. This investigation focuses on the formation of dirhenium metal:dinucleotide …
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Protein O-Kinases in the Archaeon Sulfolobus solfataricus
… membrane protein. This protein kinase utilizes purine nucleotides as phosphoryl donors <i>in vitro</i> with a requirement for a divalent metal ion cofactor, favoring Mn⁺². A preference for NTPs over NDPs and for adenyl nucleotides over the analogous guanyl nucleotides was observed. The enzyme …