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Showing 1 to 20 of 70 for “"Purified Protein"”.
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The dynamics and regulation of actin filaments in vascular endothelial cells and in a reconstituted purified protein system
… and distribution of actin monomer and polymer. Proteins that regulate actin cycling control cellular architecture and movement. One method to measure parameters that characterize actin dynamics is photo activation of fluorescence (PAF), which can simultaneously estimate the fraction of total …
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Purification and Activity of the DnaK Heat Shock Protein of the Emerging Human Pathogen Rhodococcus equi. Optimisation of methods of purifying DnaK from Rhodococcus equi, and the use of the purified protein in assays to demonstrate its activity in isolation and with other heat shock proteins
… and oxygen radicals, the role of heat shock proteins in the pathogenesis of R. equi has not been well documented. In this project we developed and optimised methods to purify the heat shock protein DnaK from R. equi, using a combination of ion-exchange and affinity chromatography. The …
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Purification and Activity of the DnaK Heat Shock Protein of the Emerging Human Pathogen Rhodococcus equi. Optimisation of methods of purifying DnaK from Rhodococcus equi, and the use of the purified protein in assays to demonstrate its activity in isolation and with other heat shock proteins
… and oxygen radicals, the role of heat shock proteins in the pathogenesis of R. equi has not been well documented. In this project we developed and optimised methods to purify the heat shock protein DnaK from R. equi, using a combination of ion-exchange and affinity chromatography. The …
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The Isolation of Native Glutaminyl Cyclase from Saccharomyces cerevisiae
… N-terminal glutamine residues of peptides and proteins. Recent interest in QC has been generated by studies that have linked QC activity in human neuroendocrine tissue to diseases such as Alzheimer's and osteoporosis. While QC has been isolated and characterized in multiple mammalian species, …
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Molecular Analysis of Roseburia Cecicola, a Motile Anaerobe, as a Foundation for Study of Bacterial Motility and Chemotaxis in the Murine Gastrointestinal Ecosystem
… its specific habitat in the mouse cecum. I purified the main protein (flagellin) of the flagellar apparatus of the organism. The purified protein was then used in raising polyclonal and monoclonal antibodies. In addition, the gene encoding the protein was cloned and sequenced.
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Purification of Calmodulin and Quantification of Its Levels in Mammary Tissue and Isolated Cells During Pregnancy and Lactation (Acini, Secretory, Myoepithelial)
Calmodulin has been purified to apparent homogeneity from lactating bovine mammary tissue. Calmodulin was purified by heat treatment and sequential anion exchange and gel exclusion chromatographies. The molecular weight of the purified protein was estimated by gel electrophoresis to be 18,000 …
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Carbonic anhydrase from Methanosarcina thermophila: proposal of a new class of carbonic anhydrases and putative roles for the enzyme in anaerobic acetate catabolism
… Methanosarcina thermophila strain TM-1 was purified> 10,OOO-fold (22% recovery) to apparent homogeneity and a specific activity of 4,900 units mg⁻¹.The gene encoding this CA was isolated fronl a partial genomic library on a 12-kb fragment and sequenced. Comparison of the deduced anlino acid …
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The penicillin-binding proteins of Bacilli
A study of the penicillin-binding proteins (PBPs) in the membranes of various species of Bacilli by dodecylsulphate/polyacrylamide gel electrophoresis followed by detection of the PBPs by fluorography has shown the presence of 3-5 such proteins depending on the species. Labelling of growing cells …
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Novel recombinant protein constructs for improved insulin-like growth factor-1 delivery
Four novel recombinant protein constructs were designed to improve the delivery of insulin-like growth factor-1 (IGF-1). The IGF-1 sequence was subcloned into a HisMax plasmid and expressed at low levels by transient transfection in 293 cells. Site-specific mutagenesis was used to insert all four …
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Recombinant expression, purification and structural characterization of aspergillus niger ring finger domain using nuclear magnetic resonance spectroscopy
e retinoblastoma binding protein 6 (RBBP6) is a 250 kDa nuclear protein which contains an N-terminal DWNN domain and a conserved RING finger domain. Previous studies determined the solution structure of the human RING finger domain using heteronuclear NMR spectroscopy. A bioinformatics analysis …
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Methoxypyrazine removal in grape juice: Development of a removal system using odorant and pheromone binding proteins coupled with bentonite fining
… of this project was to use previously identified proteins with an ability to bind to methoxypyrazines at low pH, and subsequently develop a binding assay to test the ability of these proteins to bind to and remove methoxypyrazines from grape juice. The piglet odorant binding protein (plOBP) and …
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MFSD7C is an ATP Transporter that Supports Bacterial Killing by Alveolar Macrophages in a Lipid-Rich Microenvironment
… killing is abrogated. We then examine the purified protein MFSD7C and perform a candidate substrate screen using a thermostability-shift assay. We identify ATP as a putative substrate for MFSD7C and validate that 32P ATP can be absorbed by E. coli expressing the protein. We also determine …
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Evaluation of granulysin and perforin as candidate biomarkers for protection following vaccination with Mycobacterium bovis BCG or M. bovis DeltaRD1
… of bovine homologues of the antimicrobial protein granulysin (Bo-lysin) and perforin by our group could be used as potential biomarkers for TB vaccination efficacy. In the present study we examined the kinetics of granulysin, perforin, IFNgamma and Fas-L responses to Mycobacterium bovis …
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Investigation of the potential bacterial proteasome homologue Anbu
Anbu is a bacterial protein with significant homology to the sub-units of the 20S proteasome and is predicted to be a novel bacterial proteasome. The goal of this project was to determine if the recombinant Anbu protein from Pseudomonas aeruginosa is a proteasome. Anbu from P. aeruginosa was …
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The role of copper proteins in the ammonia oxidation pathway of Nitrosopumilus maritimus
… oxidising pathways relying predominantly on proteins utilising haem c active sites. However, homologues for these proteins (or indeed genes for any proteins containing the characteristic haem c CXXCH motif) are absent in AOA, suggesting a completely different pathway in AOA. Novel …
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Site-specific labeling of cellular proteins with unnatural substrates of biotin ligases
… develop a general methodology for site-specific protein labeling with biophysical probes. We synthesized an isosteric ketone analog of biotin and discovered that BirA also ligates it to the AP with similar kinetics while retaining the high peptide specificity of the native reaction. Because …
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The Influence of the Diet of the Cow and the Effect of Heat Upon the Antiscorbutic Potency of Milk
… He had tried to feed young animals with diets of purified protein, carbohydrates, fat, and mineral and had met with failure. When the same diet was supplemented with small additions of milk, growth was promoted. It has just been stated how he called the unknown substances furnished by the milk, …
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Protein Dynamics: Studies of Adenylate Kinase Mutants From Escherichia Coli and Characterization of Adenylate Kinase Isoforms From Murine Cells. Applications of Fluorescence Spectroscopy and Microscopy
… with the gene of enhanced green fluorescence protein (EGFP) and then expressed in HeLa cells. Fluorescence images of the transfected cell showed that the AK1-EGFP was homogeneously distributed throughout the cell, while AK1beta-EGFP was mainly localized on the plasma membrane. The diffusion …
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Cloning, High-Level Expression, and Characterization of Escherichia Coli Cytochrome B(562)
… in front of the coding sequences for the mature protein. Localization studies on the overexpressed protein revealed that cytochrome b$\sb{562}$ is translocated to the periplasmic space. Similar experiments on cytochrome b$\sb{562}$-M7L (a point mutant of cytochrome b$\sb{562}$ in which one of the …
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