Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"Pulsed-field gel electrophoresis (PFGE)"”.
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Conjugation of extrachromosomal replicons of Rhodococcus erythropolis AN12
… potentials but the plasmids they encode. Pulsed-field gel electrophoresis (PFGE) revealed three previously uncharacterized megaplasmids in the genome of Rhodococcus erythropolis AN12. These megaplasmids, pREA400, pREA250 and pREA100, migrate at approximately 400 kb, 250 kb and 100 kb, …
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Virulence potential of enterobacteriaceae isolated from neonatal enternal feeding tubes
… describes the use of DNA finger printing, via pulsed-field gel electrophoresis (PFGE), to determine if the same strains were isolated on different occasions from the NICUs. Therefore indicating whether certain strains have colonised the NICUs leading to increased exposure and risk to the …
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A Genotypic Comparison of Staphylococcus lugdunensis Clinical Isolates of Diverse Geographic Origin
… using molecular typing methods, specifically, pulsed-field gel electrophoresis (PFGE), arbitrarily primed polymerase chain reaction (AP-PCR), inter repetitive sequence polymerase chain reaction (REP-PCR), and southern hybridization.|PFGE together with southern hybridization data proved superior …
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Neonatal enteral feeding tube as loci for Enterobacteriaceae colonisation and risk to neonatal health
… of the same strain. This was achieved using Pulsed-Field Gel Electrophoresis (PFGE). To determine the potential exposure of neonates to these organisms, a range of physiological and virulence related assays were undertaken; heat tolerance to 55°C, biofilm formation, capsule formation and …
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The prevention and diagnosis of central venous catheter-related infection
… surgery patients were characterised by pulsed-field gel electrophoresis (PFGE). The genetic heterogeneity of CoNS isolated from the skin was demonstrated and specific genotypes implicated in catheter colonisation. In addition, phenotypic and genotypic typing techniques were assessed for …
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Prevalence and fate of Clostridium difficile during sewage treatment, land application and composting
… identified by PCR ribotyping, toxinotyping, and pulsed field gel electrophoresis (PFGE). C. difficile was isolated from 92% (108/117) of the primary sludge samples and 96% (106/110) of the digested sludge samples, as well as 73% (43/59) of dewatered biosolids samples and 39% (25/64) of rivers …
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Characterization of Microorganisms of Interest to Homeland Security and Public Health Utilizing Liquid Chromatography/Mass Spectrometry
… protein expression in Escherichia coli and Shigella organisms. Automated charge state deconvolution, spectral subtraction and spectral mirroring were used to reveal subtle differences in the LC/MS data. Reproducible intact protein biomarkers were discovered based on their unique mass, retention …
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GENOMIC DIVERSITY IN Staphylococcus Aureus
… restriction endonucleases and subjected to pulsed-field gel electrophoresis (PFGE). Thirty-nine distinct genomic macrorestriction patterns (GPs) were identified using <em>Sma</em>I; 29 of these patterns are from methicillin-resistant <em>S. aureus</em> (MRSA).</p> <p>A dendrogram showing …
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Diversity of acinetobacter baumannii isolates from Egypt
… blaOXA-78, blaOXA-94, blaOXA-89/100. Typing with Pulsed-field Gel Electrophoresis (PFGE) showed an overall similarity at only 28.69% between the isolates, with variation in pattern for isolates with similar blaOXA-51-like genes. Typing with Multilocus Sequence Typing (MLST) identified 6 new …
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Phenotypic and transcriptomic consequences of ribosomal DNA copy number variation in Caenorhabditis elegans and Saccharomyces cerevisiae
… functional consequences of rDNA CNV have been largely understudied in metazoans and as such rDNA CNV represents a source of poorly understood genomic variation. I undertook this project to investigate possible functional and transcriptomic consequences of rDNA CNV in an animal model, the nematode …
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Pulsed-field gel electrophoresis subtyping of salmonella isolates from carcasses, lymph nodes and fecal samples of cattle from slaughter facilities in Mexico
… relationship between particular serotypes and PFGE patterns, 95 isolates from a Salmonella stock culture collection of the Food Microbiology Laboratory at Texas Tech University were analyzed; isolates were originally isolated from samples collected in Veracruz, Mexico in 2009. In addition, 33 …
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Molecular Studies of Restriction Genes in Enteric Bacteria
… two minutes using a physical mapping technique, pulsed field gel electrophoresis (PFGE).</p> <p>The KpnAI and KpnBI R-M systems were recognized in <em>K. pneumoniae</em> strain M5al and GM236, respectively. A macro-restriction map of <em>Klebsiella pneumoniae</em> GM236 was constructed in this …
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Molecular Characterization of Vancomycin-resistant Enterococci from Clinical and Surveillance Specimens
… of Tn1546-derived primer and IS primer. Pulsed-field gel electrophoresis(PFGE) of SmaI-digested genomic DNA was performed using CHEF-DRII device. The banding patterns were interpreted as described by Tenover et al. The presence of esp gene were determined by PCR for the N-terminal of esp …
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Evaluation, Development and Improvement of Genotypic, Phenotypic and Chemical Microbial Source Tracking Methods and Application to Fecal Pollution at Virginia's Public Beaches
… fluorometry, with the results cross-validated by pulsed-field gel electrophoresis (PFGE). Results at other beaches indicated that birds and/or wildlife were largely responsible for elevated enterococci levels during 2004 and 2005. The application of fluorometry proved difficult in opens waters due …
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Genetic and phenotypic diversity of bloodstream associated Staphylococcus epidermidis isolates obtained from private and public hospitals in South Africa
… cassette chromosome methicillin (SCCmec), pulsed field gel electrophoresis (PFGE) and whole genome sequencing (WGS). All 161 isolates (98 from private and 63 from public hospitals) were identified as S. epidermidis, with 91.9% (148/161) of the isolates being resistant to methicillin (i.e …
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Disinfectant Susceptibility of Mycobacterium avium
… in resistance to chlorine compared to their pulsed-field-gel-electrophoresis- (PFGE) matched environmental isolates. M. avium strains exhibited a mixed response to exposure to monochloramine. The CT99.9% values of three strains (2 clinical, 1 environmental) were between 6.3- and 23.5- times …
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The impact of the leather manufacturing process on bacterial growth
… spp. during the leather processing. The pulsed-field gel electrophoresis (PFGE) method of DNA fingerprinting confirmed that the bacterial species isolated during the leather manufacturing processes were the inoculated B. cereus and P. aeruginosa, and no alteration of the DNA of …
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Phenotypic and genetic characterization of Listeria monocytogenes from the environment of turkey processing plants
… In addition, these isolates were subtyped using pulsed field gel electrophoresis (PFGE). Moreover we investigated the prevalence among these isolates of three different cadmium resistance cadA determinants identified to date in L. monocytogenes: cadA1 (associated with Tn5422), cadA2 (associated …
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Functional analysis of plastid-encoded genes
… A new experimental protocol, based on pulsed-field gel electrophoresis (PFGE), was established to reliably assess the homoplastomic state of transformed plants. 1. Functional analysis of the ycf9 gene product: The inactivation of ycf9 in N. tabacum as well as in Chlamydomonas …
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Microbial Source Tracking in a Mixed Use Watershed in Northern Virginia
… in detergents as human-derived pollution) and pulsed-field gel electrophoresis (PFGE, a DNA fingerprinting technique) of select E. coli isolates. In order to determine the best method to classify the water-sample isolates, variation in antibiotic resistance data representation, known source …