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Showing 1 to 20 of 25 for “"Pulsed Electric Field"”.
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Pulsed electric field induced stress in plant systems
… Anwendung gepulster elektrischer Felder (engl. Pulsed electric fields PEF) in der Nach-Ernte Lagerung untersucht. Veränderungen im Gesamtpolyphenolgehalt, in der Aktivität und Konzentration ausgewählter Enzyme sowie der Einfluss auf die Permeabilisierung von Zellmembranen wurden in der …
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Investigation of yeast cell responses to pulsed electric field treatment /
… first to show that exposure of yeast cells to electric field pulses with nanosecond duration causes expression of features characteristic to caspase-dependent death. Exposure to PEF was followed by the activation of yeast metacaspases, permeabilization of the plasma membrane, and …
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Pulsed electric field treatment of Arthrospira platensis and Saccharomyces cerevisiae
Pulsed electric field (PEF) can induce irreversible electroporation in the bio-membrane and cause the death of microbial cells without significant thermal effect, which can be used for inactivation of microorganisms and facilitation lysis of microbial cells for lipid extraction. The present study …
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Nanosecond Pulsed Electric Field Effects on Cell Cycle and Apoptosis
… intracellular structures entitled nanosecond pulsed electric fields (nsPEF). NsPEF are high intensity power, low energy, very short pulses (nanosecond) that can provide a new biological tool for modulating intracellular structures and functions. In several cell types nsPEFs have been shown to …
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Pulsed electric field pretreatment of lignocellulose biomass for maximum fermentable sugar yield
… or chemical consumption and waste generation. Pulsed electric field pretreatment (PEF) has emerged as a promising method thought to enhance biomass permeability. In this particular study, a PEF pretreatment system was designed, simulated, constructed, and tested specifically for treating flax …
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Cascaded High Voltage Converter with Variable Control for Pulsed Electric Field Applications
… a pulse output voltage in order to create a pulsed electric field (PEF) to lyse algae. The proposed system is unique from any known PEF systems because it provides the ability to independently adjust peak voltage, pulse width and frequency of the output voltage. This in turn provides great …
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<i>In Vivo</i> Murine Melanoma Tumor Responses to Nanosecond Pulsed Electric Field Treatment
<p>High intensity nanosecond pulsed electric fields (nsPEF) were applied to melanoma tumors to observe functional and structural biological changes and to investigate the possible molecular mechanisms responsible. An animal model was set up by injecting B16F10 mouse melanoma cells into SKH-1 mice. …
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New Types of Morpho-Physiological Changes in Cells Exposed to Nanosecond Pulsed Electric Field
<p>Exposure of cells to a pulsed electric field (PEF) is the basis of multiple techniques and treatments. Nanosecond pulsed electric field (nsPEF) poses unique characteristics to induce subtle cellular effects while preserving cell integrity. Improving understanding of the mechanisms triggered by …
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Tissue Engineered Scaffolds and Three Dimensional Tumor Constructs to Evaluate Pulsed Electric Field Treatments
… ablation therapy. IRE uses short, high-intensity electric pulses to create pores in a cell's membrane and disrupt its stability. At a certain energy level, damage to the cell becomes too great and it leads to cell death. The particular mechanisms that drive this response are still not completely …
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Consumer Willingness-to-pay for Concord Grape Juice Treated by High-pressure Processing and Pulsed Electric Field
… including High-pressured Processing and Pulsed Electric Field have been widely discussed to boost consumption of Concord grape juice. We conducted an online survey to investigate consumers’ willingness to pay designing a discrete choice model experiment. Mixed logit models and …
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Nanosecond Pulsed Electric Field Induction of Programmed Cell Death Is Cell Type Dependent: An <i>in vitro</i> Study
<p>Nanosecond pulsed electric fields (nsPEFs) present a novel and effective method for cancer ablation by eradicating the ubiquitous cancer hallmark of apoptosis evasion and enforcing cancer programmed cell death. To develop nsPEFs as an anticancer method, a comprehensive understanding of cell …
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Determining the mechanism of pulsed electric field for improving meat quality : A thesis submitted in partial fulfilment of the requirements for the Degree of Doctor of Philosophy at Lincoln University
Pulsed electric field (PEF) is a novel non-thermal technology that has recently attracted the attention of meat scientists and technologists due to its ability to modify membrane structure and enhance mass transfer. Several studies have confirmed the potential of PEF for improving meat tenderness …
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Electrofusion of Escherichia coli with Giant Lipid Vesicles and the Compaction of Escherichia coli Nucleoid
… cells and hybrid synthetic lipid vesicles. Electric field-mediated fusion (electrofusion) is one of the most popular fusion methods owing to the simple implementation and quick inducibility of the fusion. In electrofusion, an alternating electric field is applied on cells/vesicles and …
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ELECTRIC FIELD IONIZATION OF SODIUM RYDBERG ATOMS
… bound to the atomic core that a relatively small electric field may cause the electron to be stripped from the atom. The field strength at which this ionization happens is strongly dependent on the state of the Rydberg atom. Pulsed electric field ionization serves, therefore, not only as a means …
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Algae Lysis with Pulsed Electric Fields
… examines a novel method of cell lysis through pulsed electric field (PEF) application that enables cost-effective extraction methods relative to current enzyme and sonication techniques.</p> <p>A theoretical model for cell membrane potential in the presence of electric field was developed, and …
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Modeling of Concentrated High Intensity Electric Field (CHIEF) and Its Comparison with Other Non-thermal Liquid Food Pasteurization Technologies
… nutritional functionalities. High pressure and electric field treatment are two non-thermal food treatment strategies that have been widely studied. Some representatives of non-thermal technologies that utilize high-pressure and electric field to pasteurize food products include High hydrostatic …
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Extraction of bioactive compounds from New Zealand Manuka leaves (Leptospermum scoparium) using emerging technologies
… properties consider the most promising field in which the research area can expand uses of bioactive compounds. Manuka (Leptospermum scoparium) is one of the shrubs that used medicinally by Māori. The high level of bioactive compounds in Manuka leaves is responsible for the powerful …
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Pulse electric field exposure effect on morphological properties of HeLa cells
… thesis is concerned with the investigation of pulsed electric field (PEF) towards biological cells. Biological cells selected in this study are HeLa (cervical cancer) cells. There are two parts of the study, which was involving modeling methods and experimental setup. Modeling method used …
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PEDOT:PSS Enhanced Electroporation for Cell Therapy Manufacturing
… and transduction processes. Although pulsed electric field electroporation effectively permeabilizes cell membranes to deliver payloads without the need for toxic chemical or viral transduction agents, conventional bulk electroporation devices face major challenges with cell viability, …
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