Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 17 of 17 for “"Psoralen"”.
-
An Exploration of the Feasibility of Combining Radiation Therapy with Psoralen Phototherapy
… initiated simultaneously with RT. X-ray Psoralen Activated Cancer Therapy (X-PACT) is one such approach, where diagnostics-energy kilovoltage (kV) x-ray coupled with energy modulators (phosphors) converts kV photon to ultraviolet (UV) light, which in turn activates psoralen. Radiotherapy …
-
Total synthesis of a Furan-side psoralen-thymidine monoadduct and its biochemical applications
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 1998.
-
The role of the human endonuclease VIII-like 1 and 3 in the repair of DNA replication blocking lesions
… agents. Human NEIL1 (hNEIL1) activity on psoralen generated mono-adducts (MAs) and three-stranded DNA ICLs was previously described and recently NEIL3 from Xenopus laevis was reported to repair psoralen generated MAs and apurinic/apyrimidinic site DNA ICLs. Here, complementary roles of …
-
Nucleotide Excision Repair, Crosslink Repair and Transcriptional Function of Xpa In Human Cells
… damaged DNA following exposure to UVA-activated psoralen, and investigated repair of a triplex forming oligonucleotide (TFO)-directed psoralen ICL. A TFO-directed psoralen DNA ICL was constructed in closed-circular DNA. In NER proficient human cell extracts, incisions were detected on both …
-
Techniques of RNA Isolation and Characterization in Infectious Pancreatic Necrosis Virus-Infected Chinook Salmon Embryo Cells
… A viral genomic RNA probe was labeled with psoralen-biotin and used in an attempt to detect virus-specific RNA species at designated post-infection times. Detection involved separating RNA on formaldehyde denaturing gels, northern transfer and in situ hybridization. The results confirmed the …
-
Chromatin Structure of Schizosaccharomyces Pombe: From the Lowest to the Highest Orders of Folding
… 2 bp. Equivalent results are obtained using a psoralen crosslinking method for measuring the repeat length in viable spheroplasts. That result, together with other control experiments, rules out many possible artifacts. The measured value of 156 $\pm$ 2 bp is smaller than the length of DNA …
-
Covalent end-immobilization of oligonucleotides onto solid surfaces
… oligo surface by hybridization. Photolysis of psoralen crosslinkers covalently immobilized the dsDNA molecules to the oligo surface. The covalently end-attached dsDNA formed brush-like structures where the dsDNA strand could react under conditions resembling the natural solution-state found in …
-
Design and synthesis of new potential anticancer agents and high-selective A2B antagonists
… relationships. Following this approach, psoralen derivatives, thought as DNA G-quadruplex stabilizers, were synthesized. For these compounds no biological assays were performed so far. Finally, the last part of the thesis has been dedicated to the synthesis of …
-
DNA Triplexes in chemistry biology and medicine
… by the binding of a TFO containing MeP and a 5’-Psoralen to a target duplex. This revealed the benefits of the combined modifications on the stability of the resultant triplex. “Soaking” experiments (in vivo) were also performed with this TFO on the organism C. elegans (the worms were soaked in …
-
Defining the role of endonuclease VIII-like 1 and 3 in the repair of interstrand crosslinks in cancer cells
… been found to participate in the resolution of psoralen induced ICLs. Therefore, this project set out to determine whether the modulation of NEIL1 or NEIL3 expression in cell lines lacking FANCD2, of the monoubiquitinated DNA-heterodimer protein complex FANCD2/FANCI, could significantly affect …
-
Investigating the role of NEIL3 in colorectal cancer resistance to oxaliplatin
… glycosylase has been implicated in the repair of psoralen- and AP (apurinic) site-induced interstrand crosslinks (ICLs), thus prompting these studies into the possible contribution of NEIL3 to oxaliplatin resistance in CRC treatment via its ability to unhook ICLs. Previous studies have also …
-
Mechanisms associated with Staphylococcus aureus and Acanthamoeba Interactions, and Therapeutic applications"
… m-Tetra (hydroxyphenyl) chlorin (m-THPC) and psoralen were very effective in eradicating bacteria. In antimicrobial assays, natural product eugenol derivatives demonstrated significant inhibitory activity on the growth of MRSA and exhibited dose-dependent cytotoxic effects on Acanthamoeba …
-
Molecular Mechanisms of Alternative Splicing Regulation: An Investigation of the Spliceosome Repressed by Hnrnp L on Cd45 Exon 4
… ss) to cause the skipping of CD45 exon 4. Using psoralen- and UV-crosslinking analysis, U1 snRNP and other protein components that associate with the sequences around the 5' ss within the hnRNP L-repressed spliceosome complexes were compared with the control complexes. These studies revealed that …
-
Applications of supercritical fluid technologies to the analysis of food components
… 7-methoxycoumarin, 3-phenylcoumarin, and psoralen, the apparent threshold density increased with increasing melting point. The melting or decomposition points of those compounds that were partially extracted ranged between 200 and 240°C. For those compounds that were not extracted, under …
-
Understanding cornea stem cells during homeostasis and wound healing in Xenopus
… CESCs. Here, we utilized a short treatment with Psoralen AMT (a DNA cross-linker), immediately followed by UV treatment (PUV treatment), to establish a novel frog model that recapitulates the characteristics of cornea Stem Cell Deficiency (SCD), such as pigment cell invasion from the periphery, …
-
An in vitro investigation of the effect of Khellin and UVA (KUVA) on normal and transformed human melanocytes
… problematic and numerous side effects of PUVA (psoralen and UVA) and other treatments currently in use for vitiligo, have justified the search for an alternative treatment. In this study, the use of khellin, a naturally occurring furochromone, which is similar in structure to psoralens, is …