Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"Pseudopodia"”.
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Cytopathic Effects of a Pathogenic Naegleria fowleri and an Anti-Nfa1 Antibody on Rat Microglial Cells
… kDa recombinant protein (rNfa1) that showed the pseudopodia specific localization by immunocytochemistry. On the basis of an idea that the pseudopodia-specific Nfa1 protein seems to be involved in the pathogenicity of N. fowleri, the cytopathic activity of N. fowleri trophozoites co-cultured with …
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Role of the Nfa1 Protein in Pathogenic Naegleria fowleri Co-cultured with Target Cells
… (rNfa1). Anti-Nfa1 polyclonal antibody, revealed pseudopodia-specific immunolocalization of Nfa1 protein in a trophozoite of N. fowleri. An anti-Nfa1 antibody showed a neutralizing effect on the cytotoxicity of N. fowleri trophozoites against CHO cells, much like treating an anti-Nfa1 antibody on …
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Production and Characterization of Monoclonal Antibodies against a Recombinant Nfa1 Protein Cloned from Pathogenic Naegleria fowleri
… Nfa1 protein reacted with eight McAbs showed on pseudopodia and food-cups by a fluorescent microscope. CONCLUSION: Eight hybridoma cell lines (A1F, A3C, A6D, A10F, C3A, C6F, C7F, B6B) secreting high titer of McAbs against the recombinant His-tag fusion Nfa1 protein were established. Also, the …
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Inflammation and neutrophil recruitment in ageing subjects and patients with chronic obstructive pulmonary disease
… compared with control groups. They formed less pseudopodia when migrating, and had reduced surface expression of CXCR1 and CXCR2. Inhibitory studies suggested that CXCR2 was the predominant receptor in migration to biological samples. Treating COPD cells with a PI3 Kinase inhibitor …
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A proposed role for the Ca ion in the chemotactic response of Physarum polycephalum
… control such cellular processes as extension of pseudopodia, exocytosis, endocytosis and the direction of movement (chemotactic response) of amoeboid cells. In the specific case of slime mould plasmodia, which best exemplify all of Durham's hypotheses, the simplest hypothesis to explain the …
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The effect of nfa1 accination against Naeglearia fowleri infection
… that is cloned from N. fowleri is located on pseudopodia, especially amoebic food-cups and plays an important role in the pathogenesis of N. fowleri. In this study, the retroviral vector (pQCXIN) and the lentiviral vector (pCDH) cloned with the egfp-nfa1 gene was constructed and characterized …
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Benthic protozoan community attributes in relation to environmental gradients in the Arabian Sea
… Gromia, a large marine protist with filose pseudopodia and an organic test that is abundant in the bathyal Arabian Sea. Deep-water Gromia-like morphospecies were discovered in the 1990’s but their relation to shallow-water species was not established. Little is known about gromiid diversity, …
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Characterisation of the G. mellonella cellular innate immune response and a comparison with the human neutrophil
… oxygen species production, phagocytosis and pseudopodia production. Work in this Thesis documents the separation of the G. mellonella larvae haemocyte population into sub-populations that differ to each other in size and granularity by employing Flow cytometry cell sorting techniques. It is …
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Characterization of an nf-actin gene in the pathogenic Naegleria fowleri
… the Nf-actin was localized in the cytoplasm and pseudopodia, especially, food-cup structure (amoebastome), of N. fowleri trophozoites. When N. fowleri were co-cultured with CHO cells, the Nf-actin was observed to localize around on phagocytic food-cups. When the Nf-actin protein was inhibited …
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Filopodial Dynamics and Synapse Specification in the Drosophila Visual System
How is the synaptic specificity achieved in neural circuits comprised of hundreds of different types of neurons? My dissertation aims to advance our knowledge on this overarching question using the complex visual processing circuitry of D. melanogaster. This system not only provides excellent …
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A molecular approach to questions in the phylogeny of planktonic sarcodines
… superclass Actinopoda based on their specialized pseudopodia called axopodia. Sequences from two orders of Acantharea (Symphyacanthida and Chaunacanthida) and four representatives from the order Spumellarida and the class Polycystinea (one solitary and three colonial spumellaria) were aligned …
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Probing the regulatory mechanisms of the actomyosin system in motile cells
… adopted a round shape, with no of few pseudopodia. With the help of a microfluidic cell squeezer device, I could show that Wiskostatin treated cells display a reduced mechanical stability, comparable to cells treated with the actin disrupting agent Latrunculin A. Furthermore, the WASP …
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Hyperactive Rac1 Drives MAPK-Independent Proliferation in Melanoma by Assembly of a Mechanosensitive Dendritic Actin Network
Cancer cells use a variety of mechanisms to subvert growth regulation and overcome environmental challenges. Often, these same mechanisms enable cancer cells to develop resistance to targeted therapies. Here, we describe how a hyperactivating mutation of the Rac1 GTPase (Rac1P29S) harnesses Rac1's …