Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 14 of 14 for “"Proximity-dependent"”.
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Identification of TbRP2-interacting proteins using proximity-dependent biotinylation (BioID)
… the targeting and function of TbRP2, I employed proximity-dependent biotin identification (BioID), in combination with quantitative proteomic (SILAC) techniques, to identify putative TbRP2-interacting proteins in vivo. A selected cohort of these proteins were subsequently interrogated by …
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Protein interaction studies on the titin A150 domain using proximity-dependent biotinylation
… molecular mechanism behind HMERF. In this study, proximity dependent biotinylation (PDB) was used to screen for protein interactions. PDB was conducted using the biotin-ligase UltraID fused to a titin fragment. This study encompasses the creation of the titin-UltraID fusion genes, the functional …
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Enzyme-mediated labeling of proteins and protein-protein interactions in vitro and in living cells
… two proteins interact do LpIA and LAP come into proximity, allowing probe ligation onto the peptide to occur as a readout of the interaction. We demonstrate that proximity-dependent coumarin ligation detects protein-protein interactions in living mammalian cells with extremely low background, a …
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Post-Translational Regulation of Zeb1 Contributes to Tgfβ-Mediated Emt
… Zeb1 interactions using the BioID method for proximity-dependent labeling of proteins. These results lead us to hypothesize that by targeting factors which mediate Zeb1 post-translational modification we may provide a therapeutic approach for metastasis suppression in NSCLC.</p>
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Proximity-based systems: Incorporating mobility and scalability through proximity sensing
This thesis argues that the concept of spatial proximity offers a viable and practical option for the development of context-aware systems for highly mobile and dynamic environments. Such systems would overcome the shortcomings experienced by today’s location-based and infrastructure dependent …
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The PLK1 and NLRP3 interactome in NLRP3 inflammasome activation
… mechanism of how PLK1 regulates NLRP3. Using proximity-dependent biotin identification (BioID), a method based on fusion of a promiscuous biotin ligase to a protein of interest, I identified 553 and 645 proteins as potential new interacting partners of NLRP3 and PLK1 respectively during …
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Spatially restricted proteomic mapping of excitatory and inhibitory post-synaptic termini through enzymatic labeling
… called APEX, into the synapse to enable specific proximity dependent biotinylation of the proteomes at the glutamatergic (excitatory) and GABAergic (inhibitory) postsynaptic densities. In doing so, we provide the first selective and comprehensive survey of the inhibitory postsynaptic density, …
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Novel detection and evasion mechanisms pertinent to immunity against Salmonella Typhimurium
… approach for the identification of novel DRPs by proximity-dependent biotinylation using the ascorbate peroxidise enzyme APEX. This approach preferentially labels proteins located at damaged endomembranes for subsequent identification by TMT mass spectrometry. Four enriched proteins CCDC50, …
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Enzyme mediated labeling of synaptic proteins for proteomics and imaging
… the application of peroxidase mediated, proximity-dependent biotinylation to map the proteomes of the excitatory and inhibitory synaptic clefts, which mediate distinctively different intercellular signaling between neurons; the former propagates neuronal signals while the latter inhibits …
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A Role for the HSV Tegument Protein pUL16 in Modulating Cellular Energy Metabolism during HSV-2 Infection
… Consistent with these findings, our proximity-dependent biotinylation experiments identified both mitochondrial-associated membrane and mitochondrial matrix components in proximity to HSV-2 pUL16 during virus infection. While the interaction between pUL16 and host cell mitochondria …
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Assessing Helicobacter pylori's HcpE and DsbK's Effects on Immunostimulation in Host Gastric Cells
… host potential interacting partners, BioID (proximity-dependent biotin identification) and Immunoprecipitation (affinity purification) were implemented, and candidates were identified by mass spectrometry. Finally, the subcellular localization of HcpE in host cells was assessed by …
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Discovery and functional analyses of new components of the apical complex and the pellicle of Toxoplasma gondii
… interactions of the T. gondii apical annuli, proximity-dependent biotin identification (BioID) was applied using AAP6 as bait. Two novel annuli proteins, which may be related to vesicle trafficking, named TgSnare and TgSyntaxin were discovered from the BioID study. Collectively, these studies …
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The chromatin landscape of colorectal cancer cells
… space. Transcription of the human genome is proximity-dependent requiring the cooperative engagement of non-coding elements and epigenetic modifiers to create permissive topological chromatin contacts and structures. The repertoire of chromatin contacts at any given time is regulated by the …