Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"Proximity Labeling"”.
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Directed evolution of TurboID for efficient proximity labeling in living cells and organisms
… from contaminants. Enzyme-catalyzed proximity labeling (PL) has emerged as a new approach to study the spatial and interaction characteristics of proteins in which a PL enzyme can be genetically targeted to a subcellular region and used to tag surrounding endogenous proteins with a …
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Directed evolution of split APEX peroxidase
… subcellular staining for electron microscopy to proximity biotinylation for spatially restricted proteomics and transcriptomics. While this strategy has provided access to many cellular regions and organelles, there are still many compartments and structures that cannot be accessed; this strategy …
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Development of Oligonucleotide-directed proximity-interactome MAPping (O-MAP), for characterizing RNA-protein interactions and higher order subnuclear architecture in situ
… address these problems, recent work has utilized proximity labeling as a way to study specific RNA-protein interactomes within the subcellular context; however, few methods exist to specifically target and enrich for RNA scaffolded subnuclear bodies. Utilizing existing RNA-fluorescence in situ …
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FROM MICRONUCLEI TO EMT: MOLECULAR MECHANISMS OF GENOMIC INSTABILITY AND CELLULAR PLASTICITY
… Utilizing advanced methodologies, including proximity labeling and mass spectrometry, this study reveals how p62 recognizes micronuclei through ubiquitination and proximity to mitochondria, with ROS-induced homo-oligomerization of p62 driving micronuclear envelope rupture. These findings …
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Investigating the Role of the Lysine-Specific Demethylase 4C in Pancreatic Ductal Adenocarcinoma
… pivotal effector downstream of mutant RAS. Using proximity labeling, we identified the histone deacetylase SIRT1 as a novel interacting protein with KDM4C via the latter’s Tudor reader domain. SIRT1-mediated deacetylation leads to repression of downstream targets, including the dual specificity …
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The Role of HOIL 1 Ubiquitin Ligase Activity in MDA5 Antiviral Signaling
… and more minor capacity. Using a BioID-based proximity-labeling approach, we have identified novel candidate interactors of HOIL1 induced during MDA5 signaling. In addition, we have found that LGP2 is ubiquitinated by HOIL1 during virus infection. Given the central role of LGP2 in MDA5 …
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Identifying and Inhibiting Cholesterol-Protein Interactions for Therapeutic Development
… First, we introduce a new method using Turbo ID proximity labeling paired with site-specific modulation of the cholesterol concentration to identify new cholesterol-protein interactions in the inner plasma membrane in live cells. Surface plasmon resonance is employed to further characterize …
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Comparison of wild-type and hotspot mutant p53 interactomes: The hunt for mutant-specific binding partners & Investigation and characterization of natural killer cell responses in genetically-engineered mouse models of non-small cell lung cancer
… drivers of GOF phenotypes, we used the protein proximity labeling assay BioID to interrogate differences between wild-type and point mutant p53 interactomes. We demonstrate that p53 retains function after addition of the biotin ligase miniTurbo and that known p53 binders can be identified. We …
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Development of Tools to Quantitatively Probe Mycobacterium tuberculosis-induced Host Phagosomal and Signaling Responses
… phagosome through pathogen-mediated proximity labeling. This led to the development of three strategies for chemically conjugating heterologous proteins and enzymes. Future work will apply these strategies to functionalize the surface of Mtb with proximity ligases to identify the host …
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Lysosomes and Ribonucleoprotein Dynamics in Neurons
… neurons. Within this system, we used proximity labeling proteomics to profile the lysosomal surface proteome. We discovered that annexin A11 (ANXA11), a protein linked to familial amyotrophic lateral sclerosis (ALS), is a novel component of the lysosomal surface. ANXA11 is also a …
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INTEGRATED MOLECULAR INVESTIGATIONS OF MYRF AND SFRP2: UNVEILING REGULATORY MECHANISMS OF ER STRESS AND ECM REMODELLING IN PANCREATIC CANCER
… and cleavage. Using enzyme-catalyzed proximity labeling followed by biotin pull-down and mass spectrometry, we identified MYRF proximal interactors in the ER lumen. Currently, we were able to obtain a first validation of MYRF interaction with UGGT1, which could provide quality control …