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Showing 1 to 10 of 10 for “"Proton uptake"”.
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Studies on the Proton Pumping Mechanism of Aa(3)-Type Cytochrome C Oxidase
… c oxidase was used to detect directly the proton uptake and release events during the F-to-O transition. Absorption change of the pH sensitive dye was monitored spectroscopically. Rapid proton release followed by slow proton uptake was observed. This result indicates that both the pumped …
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Computer simulation of titration behavior in proteins
… success in reproducing its known pH-dependent proton uptake. Mutants in which two critical residues in the secondary quinone binding site of the reaction center are altered are shown to be proton uptake impaired, with respect to the wild type.
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Properties of Conductance and Inhibition of Proton Channels: M2 from Influenza A Virus and Fo from Escherichia coli ATP Synthase
<p>Proton channels are essential for many of the processes of life. The influenza A viral protein M2 is responsible for sensing the conditions necessary for viral RNA release. The proton-translocating FoF1 ATPase (ATP synthase) uses a proton gradient to drive adenosine triphosphate (ATP) synthesis. …
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Biochemical Studies on the Catalytic Cycle of Cytochrome C Oxidase in Rhodobacter Sphaeroides
… from Paracoccus denitrificans reveals two proton channels leading to the binuclear center. (Iwata, S., Ostermeier, C., Ludwig, B., & Michel, H. (1995) Nature 376, 660--669) One channel is referred to as the K-channel after the highly conserved lysine-362 located within it. The other channel …
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Discovery and characterization of pentose-specific transporters in Saccharomyces cerevisiae
… sugars. Additionally, inefficient pentose uptake has been shown to be the limiting step for some D-xylose metabolizing yeast strains. In this thesis, we report the discovery of seven active pentose transporters from pentose assimilating fungal species Pichia stipitis and Neurospora crassa …
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Structure, Function and Dynamics of G-Protein coupled Receptors
… structural and thermodynamical model of coupled protonation of the conserved ERY motif in transmembrane helix 3 of rhodopsin and of helix restructuring in the micro-domain formed at the protein/lipid water phase boundary. Furthermore, synthesized peptides and full length systems were studied by …
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Relationships Between Proton Translocation, Absorption Changes, and Photocurrents in the Bacteriorhodopsin Photocycle
The mechanisms of proton release and uptake in bacteriorhodopsin (bR) were investigated using steady state and kinetic spectrophotometry and photocurrent methods. First, a kinetic model for the formation of the crucial M intermediate of the photocycle was formulated. The proposed model involved the …
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Mutagenesis investigations into proton transfer pathways and control points in respiratory complex I
… from NADH to ubiquinone to transport four protons across an energy transducing membrane. The protons contribute to building the proton-motive force that powers oxidative phosphorylation, and the oxidation of NADH is crucial for NADH/NAD+ homeostasis, positioning complex I at a key control …
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Structure-based theoretical characterisation of the redox-dependent titration behaviour of cytochrome bc1
… from coenzyme Q (CoQ) to cytochrome c to shift protons across the membrane. The chemical energy of reduced CoQ is thus converted into the energy of a proton motive force. The coupling between electron transfer and proton translocation is based on the Q-cycle mechanism. This mechanism comprises …
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Studies on the structure, function and mechanisms of the cbb3 type cytochrome c oxidase from Vibrio cholerae and the cytochrome bo3 ubiquinol oxidase from Escherichia coli
… electrons are transferred into the enzyme while protons are up taken at the same time. Some of the protons participate in the transformation of dioxygen into water as substrate. The rest of the protons are translocated across the plasma membrane, conserving energy in the form of electrochemical …