Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"Proteorhodopsin"”.
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Physiological effects of heterologous expression of proteorhodopsin photosystems
Proteorhodopsin (PR) phototrophy plays an important role in the marine ecosystem, harvesting energy from sunlight for a diverse community of hetertrophic organisms. The simple proteorhodopsin photosystem (PRPS) composed of six to seven genes is sufficient for producing a functional light-driven …
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Metatranscriptomic and physiological analyses of proteorhodopsin-containing marine flavobacteria
Proteorhodopsin (PR) is a seven-helix integral membrane protein that uses retinal as a chromophore. PRs transport protons from the cytoplasmic (CP) to the extracellular (EC) side of the cell membrane utilizing the energy from light. Since PR was first discovered in marine Gammaproteobacteria, …
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Exploring the effects of proteorhodopsin on the physiology of native and heterologous hosts
Photoheterotrophic microbes use proteorhodopsin (PR) and other types of microbial rhodopsin photosystems to harness energy directly from sunlight. This thesis explores the photophysiology of PR in the context of a natural marine isolate, Dokdonia sp. strain MED 134, and by establishing Escherichia …
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Conformational Dynamics of Rhodopsins Visualized by Time-resolved Wide Angle X-ray Scattering
… light-driven proton pumps bacteriorhodopsin and proteorhodopsin were the first membrane proteins to be characterized using TR-WAXS. The results from these studies indicated that significant α-helical rearrangements precede the primary proton transfer event in bacteriorhodopsin. Comparison with …
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Untangling Genomes from Metagenomes
… and clarifying the evolutionary origin of the proteorhodopsin gene found in numerous marine Bacteria. This genome further enabled identification and metagenomic assembly of five genomes describing the first reported non-extremophilic archaeal viruses. These genomes reveal a dramatic …
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Biohydrogen production in Escherichia coli – a synthetic biology approach
… the heterologous expression of a proton-pumping proteorhodopsin. Finally the bidirectionality of E. coli Hyd-2, i.e. H2 oxidation and H2 production, was demonstrated, suggesting that it may operate as a quinol release valve in vivo.
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Microbial production and consumption of marine dissolved organic matter
… glucosides, increased gene expression related to proteorhodopsin-based phototrophy, and decreased gene expression related to motility. Putative identification of compounds present in Prochlorococcus-derived DOM supported these responses. Collectively, these findings highlight the potential for …
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Microbial production and consumption of marine dissolved organic matter
… glucosides, increased gene expression related to proteorhodopsin-based phototrophy, and decreased gene expression related to motility. Putative identification of compounds present in Prochlorococcus-derived DOM supported these responses. Collectively, these findings highlight the potential for …
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Structural and Biophysical Characterisation of Denatured States and Reversible Unfolding of Sensory Rhodopsin II
… studies have demonstrated successful assembly of proteorhodopsin from two separate fragments consisting of helices A–B and helices C–G via a splicing site in the BC loop. To complement the in vitro unfolding/folding studies, pSRII assembly in vivo was attempted by introducing a splicing site in …