Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 41 for “"Proteomics analysis"”.
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Quantitative proteomics analysis in cultured skin fibroblasts from patients with rare genetic disorders
… are used as a cell model for the disease. A proteomics quantification method is applied to study the pathogenic alterations in these cells.
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Quantitative Proteomics Analysis of Differentially Expressed Proteins in Aβ(17-42) Treated Synaptosomes
… treated synaptosomes and control. By using proteomics, we have also identified several biomarkers including aldehyde dehydrogenase, aldolase, α-enolase, heat shock cognate 71, peptidyl-prolyl cis-trans isomerase, and ATP synthase α chain. Our present findings, suggest the role of Aβ(17-42) …
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MATRIX-ASSISTED LASER DESORPTION/IONIZATION (MALDI) TARGET MODIFICATION FOR ENHANCED PROTEOMICS ANALYSIS AND PLASMA POLYMER CHARACTERIZATION BY MALDI MASS SPECTROMETRY
… from complex samples. For applications involving analysis of complex peptide/protein mixtures, fractionation of the mixture on the basis of component pI can reduce MALDI ion suppression effects leading to efficient ionization of larger numbers of mixture components. In the present studies a MALDI …
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Physico-chemical characterization of the native and mutant protein cochlin, and its role in adult-onset hearing and balance loss
… chromatography, the Bradford assay, western blot analysis, and proteomics analysis by mass spectroscopy, I acquired data which suggests that the concentration of cochlin in the perilymph of bovine calf ears is at most on the order of 10- mg/ml. I also determined the total protein concentration of …
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Development of ion mobility mass spectrometry deconvolution strategies for use in data-independent acquisition proteomics.
… powers can limit the success of MS-based proteomics approaches. This work highlights the development of IM-MS deconvolution techniques and their utilization with commercial data processing software to improve results from IM-enhanced data-independent acquisition (HDMSᴱ) proteomics …
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NUTRITION AND IMMUNITY: MOLECULAR APPROACHES
… vitro functional analyses and OMIC technologies (proteomics and miRNAomics) were implemented herein for an integral characterization of the molecules’ impact on the animals’ immune response. Specifically, in this thesis the in vitro impact of the n-6 conjugated linoleic acid (CLA), citrus pectin …
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Nephrotic Syndrome and Glomerular Basement Membrane: Genetic Defect of the Laminin α5 Chain
… using <i>in vitro</i> studies, extensive proteomics analysis and investigation of integrin activation, revealed a possible impact of the causative mutation on protein folding. Data suggest that changes in protein structure lead to a reduced secretion, integrin β1 activation, and agrin …
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A network analysis based proteomic and transcriptomic investigation into HIV-Tat induced neuronal dysfunction and the neuroprotective effect of lithium
… achieve this goal, deep, quantitative proteomic analysis of HIV-Tat treated SILAC-labelled SH-SY5Y neuroblastoma cells was carried out, alongside transcriptomic analysis of the same system in which 3077 proteins were identified and quantified with 407 proteins and 1074 genes being differentially …
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The role of recombinant human laminin-111 in adhesion-signaling and glycosylation in laminin-⍺2 deficient muscle
… to develop therapeutics for LAMA2-CMD. Spatial proteomics analysis of LAMA2-CMD patient biopsies revealed altered biological processes including protein aggregation, oxidative stress, and dysregulated glycolysis. In the LAMA2-CMD mouse model, the dyW, spatial proteomics showed similar altered …
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Twist of messenger Fate: novel mechanisms for TDP43 in modulating mRNA decay and alternative polyadenylation
… specific motor neuron death? Our quantitative proteomics analysis of the TDP43 interactome revealed the interaction with components of the mRNA surveillance pathway, suggesting a still undiscovered function in nonsense-mediated decay. We demonstrated that TDP43 acts translation- and …
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The HV1 proton channel of Lingulodinium polyedrum localizes to the bioluminescent scintillon
… is present in this organelle. In addition, proteomics analysis demonstrated that isolated scintillon preparations contain peptides that map to LpH<sub>V</sub>1, including a portion of the epitope used to raise the antibody. These results indicate that LpH<sub>V</sub>1 is the voltage gated …
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Comprehensive Proteomic Analysis and Characterization of Human Bone Marrow Mesenchymal Stem/Stromal Derived Extracellular Vesicles
… composition of hBM-MSC-EVs using a quantitative proteomics analysis. hBMMSC-EVs were produced from 5 hBM-MSC donors characterized by Nanoparticle Tracking Analysis showed no differences in the hBM-MSC-EV concentration and size among the 5 donors (1.83 x 10¹⁰ ± 3.23 x 10⁹ /mL), with the mode …
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Interaction between Leishmania parasites and mammalian macrophages
… The information obtained from comparative proteomics analysis of the infection confirmed that Leishmania regulates apoptotic processes. On the basis of the results obtained a model was presented to explain how induction of apoptosis allows intracellular amastigotes to spread unrecognised to …
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A proteomic investigation of the rhizomes of the resurrection fern Mohria caffrorum L. (Desv.) in response to desiccation
… and viability studies using electrolyte leakage analysis showed that the rhizomes were desiccation-tolerant throughout the seasons. Quantitative proteomics analysis using isobaric tags for relative and absolute quantification was employed to investigate molecular mechanisms of …
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Uncovering the Role of 5'-Nucleotidases in Pyrimidine Metabolism in Trypanosoma brucei
… between cytosine and uracil pools. Metabolomics analysis revealed depletion of de novo pathway intermediates, including glutamine, UDP-sugars, and CDP-phospholipids, contributing to parasite death. Isotope uptake assays showed deficiency in glutamine uptake, and proteomics analysis identified two …
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Optimization and Validation of the Derivation and Use of HoxB8 Transgenic Murine Phagocytes in In Vitro Studies
… flow cytometry, mass spectrometry-based proteomics analysis, and comparison of key phagosomal parameters (phagocytic index, phagosomal maturation (intra-phagosomal proteolysis), and phagosomal NOX2 activity). I also demonstrated the genetic mutability of macrophages derived from HoxB8 …
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Imagining the Internal Reproductive Environment: Function and Evolution of the Drosophila Melanogaster Female Reproductive Tract
… functions. In my second chapter, I conducted a proteomics analysis of the female reproductive tract that demonstrates the unique composition and response to mating of the extracellular female reproductive tract fluid as compared to the intracellular tissue contents. The distinct characteristics …
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A Proteomics Approach to Investigate Uropathogenic Escherichia coli
… with a focus on ST127, using a quantitative proteomics approach. We employed a classical shotgun proteomics approach to analyse proteins extracted from multiple strains of UPEC following growth in various environments (Lysogeny broth, artificial urine medium and co-culture with uroepithelial …
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Computational Modeling of Planktonic and Biofilm Metabolism
… approaches (e.g., transcriptomics and proteomics analysis). However, these approaches are limited to provide the direct description of intracellular metabolism (e.g., metabolic fluxes) of microorganisms. Therefore, in this study, I have applied computational modeling approaches (i.e., …
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