Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 27 for “"Proteomic techniques"”.
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Utilizing Proteomic Techniques to Discover Host Protein Interactions with the E1 Glycoprotein of Venezuelan Equine Encephalitis Virus (VEEV) for Anti-Viral Discovery
Venezuelan equine encephalitis virus (VEEV) is an alphavirus that causes disease in humans and equines eliciting both an agricultural and public health threat. In humans, the disease typically presents as a febrile illness with common signs of fever and malaise. Four to fourteen percent of …
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Detection and enrichment of cytochrome P450s using bespoke affinity chromatography and proteomic techniques. Development of chemical immobilisation and novel affinity chromatography methods, with subsequent proteomic analysis, for the characterisation of cytochrome P450s important in cancer research.
… develop enrichment procedures for the P450s, a proteomic strategy was developed so that compounds could be screened for their effectiveness as general P450 probes. A standardised work-flow was created, encompassing affinity chromatography, protein concentration/desalting, followed by sodium …
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A methodological investigation into the roots of the resurrection plant, Xerophyta viscosa, for further proteomic analyses
In order to conduct proteomic analysis on the hydrated root tissues of the resurrection plant, Xerophyta viscosa Baker, aeroponically grown plant roots were subjected to various proteomic techniques. Three protein extraction methods were investigated, of which one method, was the most suited in …
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Proteomic analysis of diurnal variation in human skeletal muscle performance
… more sophisticated analysis is required. Proteomic techniques including 2-dimensional gel electrophoresis, high- performance liquid chromatography and peptide mass spectrometry can be used to investigate changes in the abundance of hundreds of proteins simultaneously. To date, such …
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Functional characterization and expression of molluscan detoxification enzymes and transporters involved in dietary allelochemical resistance
… as substrates for these proteins. Molecular and proteomic techniques identified both allelochemically-responsive CYPs, and constitutively expressed GSTs and transporters in Cyphoma digestive glands. Inhibition of Cyphoma GST activity by gorgonian extracts and selected allelochemicals (i.e., …
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Neue Methoden der Proteinanalytik zur Identifikation von diagnostisch relevanten Molekülen für das Harnblasen- und Prostatakarzinom
… respectively, were subjected to different proteomic techniques (2D-SDS-PAGE, MALDI- and SELDI-TOF-MS, MALDI imaging). In particular, analysis of magnetic bead based fractioned serum samples of both entities resulted in sensitivity and specificity values above the standard values when …
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The Role of Proteomics in Colorectal Cancer Biomarker Discovery
… there has been increased expansion in the use of Proteomic technologies to undertake such research. The primary aim of this thesis was to apply proteomic techniques to analyze serum of patients with CRC, and thus develop novel biomarkers to aid in the diagnosis of CRC within a screening …
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Emerging concepts in the pathophysiology of acute pancreatitis
… mesenteric lymph (ML). Using the advanced proteomic techniques of isobaric tags (iTRAQ™) for relative protein quantitation together with liquid chromatography–tandem mass spectrometry for the identification of the component proteins, the first comprehensive description of the normal (fasted …
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Functional characterization and expression of molluscan detoxification enzymes and transporters involved in dietary allelochemical resistance
… as substrates for these proteins. Molecular and proteomic techniques identified both allelochemically-responsive CYPs, and constitutively expressed GSTs and transporters in Cyphoma digestive glands. Inhibition of Cyphoma GST activity by gorgonian extracts and selected allelochemicals (i.e., …
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Characterization of the White-rot Fungus, Phanerochaete carnosa, through Proteomic Methods and Compositional Analysis of Decayed Wood FibreCharacterization of the White-rot Fungus, Phanerochaete carnosa, through Proteomic Methods and Compositional Analysis of Decayed Wood Fibre
… of the corresponding secretome using proteomic techniques. Through this study, cultivation methods for P. carnosa were established and biochemical assays for protein activity and quantification were developed. Analytical methods, including FTIR and ToF-SIMS were used to characterize …
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The role of individual protein turnover in skeletal muscle adaptation
… combining deuterium labelling and advanced proteomic techniques with computational biology, to investigate muscle protein dynamics at the individual protein level. We have used programmed exercise to perturb skeletal muscle in vivo for the purposes of studying two contrasting types of muscle …
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Characterisation and modulation of drug resistance in lung cancer cell lines
… cancer cells while developing siRNA and membrane proteomic techniques and to increase our knowledge of the effect of lapatinib, a newly developed targeted therapy, in these resistant cells. Lapatinib, a growth factor receptor tyrosine kinase inhibitor synergised with P-gp substrate cytotoxics in …
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The identification of biomarkers of chemotherapy resistance in breast cancer using comparative proteomics
… associated with chemotherapy resistance, using proteomic analysis of fresh breast cancer tissue samples.Materials and Methods:Chemotherapy-sensitive (CS) and chemotherapy-resistant (CR) tumour samples were collected from breast cancer patients who received neoadjuvant therapy consisting of …
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Characterisation of novel and complex mechanisms of antobiotic resistance using a proteomics approach
… mechanisms that confer antibiotic resistance. Proteomic techniques allow the global analysis of the expressed proteome, providing a more holistic view of the current physiological state of the bacterial cell. The techniques used in this investigation cover the separation, quantification and …
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Copper as an Antibacterial Agent and Disruptor of Protein Stability
… a variety of biological, spectrometric, and proteomic techniques to study how Cu and a small molecule ionophore called pyrithione (PT) kill bacteria. First, we present antibacterial susceptibility assays that demonstrate PT and a β-lactamase-activated prodrug of PT called PcephPT kill …
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Antibody technology identifies distinct mechanisms controlling the lengths of filopodia-like structures
… and immunostaining approaches with different proteomic techniques and transient expression. The antibody panel was strikingly split into two groups: (1) Antibodies that lengthen FLS and do not recognise specific proteins in their eluates or (2) Antibodies that shorten FLS and show specific …
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Estudio genómico, transcriptómico y proteómico del proceso de infección del hongo fitopatógeno botrytis cinerea
… emphasis is placed on the application of omics techniques, such as genomics and proteomics, to better understand the molecular mechanisms employed by B. cinerea during its infection cycle and the use of endophytic microorganisms for its biological control. In the first chapter, the genes BcPKS2, …
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Investigation of protein biomarkers in HIV positive and HIV negative associated DLBCL
… subtype (p value=1,47738). 10 Conclusion: Using proteomic techniques, we identified and visualized differentially expressed protein in DLBCL subtypes and controls. The majority of these proteins belonged to glycolysis, ATP synthesis, and cellular movement.
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Studies on equine milk and comparative studies on equine and bovine milk systems
… the stabilization of equine casein micelles and proteomic techniques support this view. In this study, equine κ-casein appeared to be resistant to hydrolysis by calf chymosin but equine β-casein was readily hydrolysed. Resolution of equine milk proteins by urea-PAGE showed the …
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Investigation of the intrinsic mechanism of drug resistance in multiple myeloma
… bortezomib resistance in cellular models using proteomic techniques and isolated and identified several novel proteins which may play a role in this phenomenon. Our findings are mechanistically consistent since two of the identified proteins Hsp70 and caspase-3 are known in the literature to be …
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