Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 53 for “"Proteomic profiling"”.
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Differential proteomic profiling towards elucidation of TB-IRIS pathogenesis
… (HPLCMS/MS) is well-suited to differentially profiling the ex vivo neutrophil proteome in an unbiased manner, in order to investigate TB-IRIS predisposition and pathogenesis. Methods Applying first principles to existing human literature, the most parsimonious holistic hypothetical model …
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Proteomic Profiling of Serum Derived Exosomes from Prostate Cancer Patients
… exosomes in PCa and a qualitative profile of its proteomic composition. The higher levels of circulating exosomes in sera of PCa patients is directly prostate-derived and could be stress-induced as the non-cancer prostate stroma and the immune system interact with neoplastic cells. This could be …
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Comprehensive proteomic profiling of clinically relevant strains of Mycobacterium tuberculosis
Tuberculosis is an airborne infectious disease caused by the bacillus known as Mycobacterium tuberculosis. Despite limited genetic variability, Mycobacterium tuberculosis strains exhibit vast discrepancies in phenotypic presentation in terms of virulence, elicited immune response and …
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Proteomic Profiling of the mdx Animal Model for Duchenne Muscular Dystrophy
… The aim of this project was to create a detailed proteomic profile of differentially affected dystrophic tissues using the mdx mouse model; from severely dystrophic diaphragm; moderately affected hind limb to naturally protected interosseus muscle were used to investigate the pathogenesis of the …
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Proteomic Profiling of Animal Models of Myotonia and Motor Neuron Disease
… tissues architecture, metabolism and function. Proteomic profiling of two very different diseased states has allowed the identification of muscle transformation occurring in opposite directions. Myotonia a common feature found in myotonic dystrophies is characterized by skeletal muscle membrane …
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Proteomic Profiling of Animal Models of Motor Neuron Disease and Muscular Dystrophy
Proteomic profiling plays a decisive role in the identification of novel biomarkers of neuromuscular disorders and the elucidation of new pathobiochemical mechanisms that underlie these diseases. Detailed mass spectrometryQbased analysis of various diseased muscle groups from animal models of motor …
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Multiplexed, scalable, and functionality compatible platforms for 3D spatially resolved proteomic profiling
… role in the whole biomolecular network. Spatial proteomics serves as an indispensable tool for extracting and deciphering cellular and molecular information of proteins, one of the important type of functional biomolecules, within biological systems. However, compared with high throughput …
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Method development and proteomic profiling of boar seminal plasma using mass spectrometry-based workflows
… develop and optimise a mass spectrometry-based proteomic method suitable for profiling boar seminal plasma. To accomplish this aim, gel-based and in-solution methods were evaluated for depth-of-coverage, reproducibility and throughput. For the gel-based method, one-dimensional sodium dodecyl …
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Oncoproteomic applications for detection of breast cancer. Proteomic profiling of breast cancer models and biopsies
… types of breast cancer. New developments in proteomics have provided the opportunity to look at phenotype-specific breast cancer cell lines and stage-specific liquid biopsies (nipple aspirate fluid [NAF], plasma samples) to identify disease and phenotype specific signature. An 8-plex iTRAQ …
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Comparison of Immune Correlates, Age Related Changes and Proteomic Profiling of Healthy Individuals Receiving Influenza Vaccines
<p>The burden of influenza related infections is substantial, both in terms of illness, lives lost and economic impact on society. The degree of impact of influenza related infections is much higher in the elderly population where it is a leading cause of catastrophic disability; greatly affecting …
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Proteomic profiling of skeletal muscle tissue from the Goto-Kakizaki rat model of type 2 diabetes.
The primary features of type 2 diabetes (T2D) are both insulin resistance and impaired beta cell function. Abnormal glucose handling and variable degrees of peripheral insulin resistance characterise T2D. Skeletal muscle is the largest insulin-regulated glucose sink in the body and represents …
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Proteomic profiling of Nguni cattle liver tissue using gel and Gel-Free approaches: methodology development and potential applications
… breeds under unfavourable conditions. Using proteomics, with mass spectrometry at the core of the analysis, the objective of this study was to establish a reliable set of methods for the protein profiling of Nguni cattle livers. To achieve this several alternative technologies were employed …
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Application and integration of bioinformatic strategies towards central and peripheral proteomic profiling for diagnosis and drug discovery in schizophrenia
Proteomic profiling studies of schizophrenia have the potential to shed further light on this debilitating and poorly understood condition which affects up to 1% of the world’s population. However, recent studies suggest that the field of proteomics in general has been hindered by poor application …
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Unbiased global proteomic profiling of patient-derived meningiomas of all grades to identify molecular signatures of differentially expressed proteins and phosphoproteins.
… of these tumours. In order to elucidate the proteomic profiling of meningiomas and identify proteins involved in their pathogenesis, we completed a comparative mass spectrometry analysis of meningioma tissue of all WHO grades, analysing global proteins, phosphoproteins and phosphopeptides. We …
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Proteomic profiling of matched normal and tumour tongue biopsies from smokers and non-smokers. Oncoproteomic applications for oral tongue squamous cell carcinoma biomarker discovery
… causative agents, specific to smoking. An iTRAQ proteomic approach was used to profile protein changes in matched normal and tumour samples from male non-smoking (n=6) and smoking patients (n=6) with tongue carcinomas revealing identification of potential targets specific to cancer. Samples were …
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Proteomic profiling of matched normal and tumour tongue biopsies from smokers and non-smokers. Oncoproteomic applications for oral tongue squamous cell carcinoma biomarker discovery
… causative agents, specific to smoking. An iTRAQ proteomic approach was used to profile protein changes in matched normal and tumour samples from male non-smoking (n=6) and smoking patients (n=6) with tongue carcinomas revealing identification of potential targets specific to cancer. Samples were …
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Quantitative Proteomic Methodology Use and Development to Characterize Ethanol Modulation of Microglial Function
… cell lines and in vivo enriched microglia. Our proteomic profiling and subsequent validation suggests that microglia do activate in response to ethanol exposure, but the activation falls short of the classical, or M1 state of inflammatory activation, as no downstream markers for reactive species …
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3D chromosome structure and chromatin proteomics
… on developing an approach called "chromatin proteomic profiling" to identify protein factors associated with various active and repressed portions of the genome marked by specific histone modifications. The histone modifications assayed by chromatin proteomic profiling are associated with …
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Proteomic Profiling of Pro and Active Matrix Metalloproteinases using Tandem Mass Spectrometry. Optimization of Affinity Chromatography and nHPLC-MALDI-MS/MS for Proteomic discrimination of Matrix Metalloproteinases in pre-clinical Cancer Model.
… of this project is to develop a chemical proteomics strategy based on Matrix assisted laser desorption ionization tandem mass spectrometry (MALDI-MS/MS) to help identify and discriminate the various MMP forms. Firstly, a triazine dye-based ligand immobilized on chromatography beads was …
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Disrupting Tymp-Mediated Pathways to Block Lymph Node Metastasis in Ovarian Cancer
… Utilizing comparative transcriptomic and proteomic profiling of primary tumors, LN metastases, and omental metastases, we identified significant upregulation of thymidine phosphorylase (TYMP) in LN metastases compared to primary and omental tumor sites. Functional studies in murine models …
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