Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 66 for “"Proteolytic cleavage"”.
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Transthyretin Amyloidosis: Proteolytic cleavage accelerates G53A TTR misfolding and aggregation
The proteolytic cleavage of the peptide bond Lys48-Thr49 of the CD loop in TTR pathological mutants S52P and E51_S52dup was previously demonstrated to promote aggregation as a result of tetrameric structure destabilization. Type A and B fibrils were detected in vivo, suggesting alternative TTR …
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Properties of the products of proteolytic cleavage of bovine colostral immunoglobulin G (igG ).
Dept. of Biological Sciences. Paper copy at Leddy Library: Theses & Major Papers - Basement, West Bldg. / Call Number: Thesis1974 .F24. Source: Masters Abstracts International, Volume: 40-07, page: . Thesis (M.Sc.)--University of Windsor (Canada), 1975.
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Investigating the Post-Translational Modification of Notch1 Intracellular Domain: From Proteolytic Cleavage to Mass Spectrometric Profiling
… domain (N1ICD), focusing on its potential proteolytic processing, post-translational modification (PTM) landscape, and associated protein interactors. In the first part of the study, the role of NRDc, a zinc-dependent metallopeptidase, in mediating cryptic cleavage of N1ICD, particularly at …
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The Alpha7beta1 Integrin in Skeletal Muscle and Muscular Dystrophy
… I report the discovery of a species-specific proteolytic cleavage of the alpha7 integrin chain and demonstrate this cleavage regulates integrin mediated cellular functions. The potential involvement of this proteolytic cleavage in muscular dystrophy is also discussed. Together, this …
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The Processing of Amyloid Precursor Protein in the Central Nervous System of Humans and Rhesus Macaques
… will be released first, and with a subsequent cleavage, β-amyloid: Aβ), will be released into the brain where it could sequester into amyloid plaques, which are a major hallmark of AD. The other processing pathway is caused by cleavage of APP by α-secretase. This results in the release of …
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Angiotensin-(1-7) Reduces Prostate Cancer Growth and Metastasis by Altering the Tumor Microenvironment
… is produced in the circulation and in tissues by proteolytic cleavage of angiotensin I or II. The heptapeptide mediates biological responses by activating a unique G-protein-coupled angiotensin-(1-7) [AT(1-7)] receptor mas, thereby providing specific targeted actions when used as a therapeutic …
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The Role of TIMP3 in Models of Inflammation and Immunity
… an important role in regulating these molecules. Proteolytic cleavage can not only destroy some molecules but can activate or shed others, converting local juxtacrine signalling proteins into effectors that act at a distance. Shedding can also convert membrane-bound receptors into soluble …
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Recombinant Expression In Pichia pastoris System Of The Potassium Channel Inhibitor Peptide, Ts6.
… N-terminal was his-tagged and an enterokinase cleavage site was inserted. The X-33 Pichia clones were cultured in increasing concentration of ZeocinTM. After methanol induction, protein expression yielded around 4.7 mg/l Ts6. The purification of the his-tag Ts6 was done in two steps that …
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Determining the mechanism of dichloroacetate-regulated processing of the Alzheimer’s disease-related amyloid precursor protein
… These fragments are formed through the proteolytic cleavage of the amyloid precursor protein (APP) by β- and γ-secretases. However, in an alternate non-amyloidogenic pathway, α-secretase (ADAM10) cleaves APP within the A-domain thereby precluding the formation of intact toxic peptides. …
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Engineering the Nicotiana benthamiana secretory pathway for the production of Lujo virus vaccines and diagnostic tools
… translational modifications glycosylation and proteolytic cleavage in recombinant HEK293T cells and was produced in a conformation that allowed successful purification via a His tag sequence that was inserted into the LUJV GP-C∆TM protein gene sequence. On the other hand, N. benthamiana does …
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Use of the cellulose-binding domain of a cellulase from cellulomonas fimi for affinity purification of fusion preteins
… water elution. The CBD was removed by specific proteolytic cleavage with Factor Xa or by a C. fimi serine protease. CBD fusions with human interleukin 2 (IL2) were constructed, but are predominantly insoluble and extensively degraded on expression in E. coli. However, the fusion polypeptides can …
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Aggrecan fragments as biomarkers in osteoarthritis
… Matrix components released into body fluids by proteolytic cleavage can be used as biomarkers of ongoing processes. This thesis focuses on proteolytic degradation of the proteoglycan aggrecan with the overall aim to study the potential of aggrecan fragments as biomarkers in knee OA. …
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Role of the interaction of proHB-EGF with heparan sulfate proteoglycans
… form of the protein (sHB-EGF), produced after proteolytic cleavage of the transmembrane form (proHB-EGF) from the cell surface. It was hypothesized that HSPGs interact with proHB-EGF in ways that could alter behavior of the transmembrane form of this ligand and consequent processes. Using an …
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Analysis of plasminogen binding to Treponema denticola, a key periopathogen
… FH serves as cofactor in the Factor-I mediated cleavage of C3b and accelerates the decay of the C3 convertase complex, leading to downregulation of C3b production. Several pathogens bind FH, and a number of these bacterial binding proteins have been shown to bind plasminogen. Plasminogen is a …
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Characterization of the Ubiquitin N-end Rule Pathway in Arabidopsis
… of candidate substrates is generated after their proteolytic cleavage by a bacterial effector protein. The transient expression of these candidate N-end rule substrates in tobacco coupled with pathogen inoculation and biochemical methods led to the identification of a group of protein fragments …
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Inhibition of Cell Proliferation through Regulated Intramembrane Proteolysis of CREB3L1
… Nile virus (WNV), and Sendai virus, CREB3L1 was proteolytically cleaved, allowing its NH2 terminus to enter the nucleus and induce multiple genes encoding inhibitors of the cell cycle to block cell proliferation. Consistent with this, we observed a necessity for CREB3L1 expression to be silenced …
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Antimicrobial and immuno-modulatory peptides as a treatment for lung disease and delivery by nebulisation
… revealed the peptides were susceptible to proteolytic cleavage. Cleavage predictions highlighted neutrophil elastase (NE) as most likely responsible. All peptides were susceptible to cleavage by NE, and several peptides retained function following NE-incubation. Mass spectrometric analysis …
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Design and Synthesis of a Novel Neuroprotective Brain-Targeting Amylin Receptor Antagonist
… mass spectrometry and reversed-phase HPLC. The proteolytic stability of R16 was evaluated in human and mouse sera to determine the half-lives and proteolytically labile sites. The half-life of R16 was found to be 8.98 hours and 0.47 hours in human and mouse serum, respectively. The N-terminal of …
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Cloning and characterization of the 3' terminal regions of RNA from select strains of maize dwarf mosaic virus and sugarcane mosaic virus
… viruses. Homology studies identified the site of proteolytic cleavage between the nuclear inclusion II proteins and the coat proteins of these viruses. Additionally, oligodeoxynucleotide directed site specific mutagenesis alteration of the MDMV-B cDNA clone allows excision of the NI$\sb{\rm II}$ …
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Cloning and Characterization of Replication Protein A from Dictyostelium discoideum
… to posttranslational modification, most likely, proteolytic cleavage. The transcription start site for both sizes of DdRPA1 has been identified at 306 bp upstream of the coding sequence by primer extension reaction. A PCR fragment representing 27% of the gene encoding the DdRPA middle size …
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