Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"Proteolytic Digestion"”.
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Papain-papaya protein interactions : resistance of papaya protein to proteolytic digestion by papain
<p>The proteolytic actjvity of papain was investigated with respect to its action on both natural substrates (native papaya protein and bovine serum albumin) and artificial synthetic substrate (benzoyl~l-arginine ethyl ester). Following removal of 30 percent and 40 percent of the amino acid …
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Tick toxinology : isolation and characterisation of the toxin from the Australian paralysis tick, Ixodes holocyclus
… was restricted to peptides generated by proteolytic digestion with trypsin as direct N-terminal sequencing revealed an amino-terminal blockage. An accurate molecular weight of 5,640 (HT-II) and 5,460 (HT-I) Daltons has been determined by mass spectrometry for two of the neurotoxins. The …
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Understanding the Molecular Level Interactions of Cancer Inhibitor Imatinib with Human Fibroblast Growth Factor-1
… unfolding using steady state fluorescence and proteolytic digestion assay. These data show that imatinib binds to hFGF-1 and enhances its thermal stability and solvent accessibility. In addition, biacore analysis was carried out to determine the binding affinity of imatinib to hFGF-1.</p>
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Identification And Characterization Of The Hexose Transporters In Rat L6 Myoblasts
… kDa) were associated with both HAHT and LAHT. Proteolytic digestion studies indicated that the external surface of the hexose transporter was resistant to trypsin cleavage, and that the CB binding sites might be located on the cytoplasmic side of the hexose transporter. Immunoblotting studies …
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Two-Dimensional and High-Throughput Electrophoretic Separation of Proteins Using Polymeric Microchips
… to mass spectrometry of peptides generated via proteolytic digestion of the proteins. For 2-D separations, microfluidic devices are superior to bench top and capillary-based systems since they potentially provide higher separation efficiencies due to the minimal dead volumes produced during peak …
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Mechanism of action of a tumour derived lipid mobilising factor
… attenuated by co-incubation with 10μM SR59230A. Proteolytic digestion of ZAG and LMF followed by sephadex G50 chromatography yielded active fragments of 6-15kDa, indication the entire molecule was not required for bioactivity. Cachexigenic MAC16 cells demonstrated significant in vitro ZAG …
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Study of zein protein body formation in a heterologous system (<i>Xenopus laevis oocyte</i>)
… 27K zein. This study utilized physical assays of proteolytic digestion (proteinase K) and chemical modification (biotinylation) on isolated protein vesicles from <i>Xenopus</i> oocytes injected with <i>in vitro</i> transcribed 27K zein mRNA. In addition, the transmembrane features were analyzed by …
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Integrating Micro-Scale Separations to Matrix Assisted Laser Desorption and Ioniation Time of Flight Mass Spectrometry (MALDI-TOF-MS) for Protein Analysis
… denaturation, protein separations, proteolytic digestion and chromatographic separations of peptides prior to MALDI TOF MS analysis. Therefore, coupling microfluidics devices to biological mass spectrometry is the first logical step towards developing fully integrated and automated …
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A Characterization of the Effect of Apolipoprotein AII on the Structure and Function of Apolipoprotein AI in Reconstituted High Density Lipoproteins
… take place to allow apoAII binding. Limited proteolytic digestion of AIrHDL with trypsin produced an N-terminal apoAI fragment of approximately 14 kDa, indicating residues in the central region of apoAI are more exposed to proteolysis after apoAII binding. The addition of apoAII to AIrHDL and …
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Molecular approaches to increasing resistance of wheat (Triticum aestivum L.) towards two insect pests; Cereal aphid (Sitobion avenae F.) and Wheat bulb fly (Delia coarctata Fallen).
… endogenous resistance factors in wheat on digestion, nutrition and other insect metabolic processes. The aim was to develop new strategies for crop protection. Digestive biochemistry in S. avenae and D. coarctata was studied to characterise gut proteases and their inhibition by host plant …
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Microfluidic Approaches for Probing Protein Phosphorylation in Cells
… and evaluated a miniaturized strategy for rapid proteolytic digestion and phosphopeptide enrichment; (3) We demonstrated sensitive detection and quantification of phosphopeptides from complex biological samples using multiple reaction monitoring mass spectrometry (MRM-MS) and microfluidic sample …
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The molecular characterisation of pregnancy-associated plasma protein-A (PAPP-A).
… combined with the sequence obtained from limited proteolytic digestion of PAPP-A were used to design oligonucleotide primers specific for PAPP-A. These primers were used in a PCR reaction that produced 500 and >1200 bp fragments using the cDNA library as DNA template; thus demonstrating that …
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Optimized GeLC-MS/MS for Bottom-Up Proteomics
… study focuses as first on the improvement of the proteolytic digestion of proteins in in-gel based proteomic approach (Gel-LCMS). To this end commonly used bovine trypsin (BT) was modified with oligosaccharides in order to overcome its main disadvantages, such as weak thermostability and fast …
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Homology-Based Functional Proteomics By Mass Spectrometry and Advanced Informatic Methods
… by mass spectrometry analysis have employed proteolytic digestion, fragmentation of resultant peptides, and the correlation of acquired tandem mass spectra with database sequences, relying upon exact matching algorithms; i.e. the analyzed peptide had to previously exist in a database in …
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Ceramidsynthese in Saccharomyces cerevisiae
… within a transmembrane domain. A limited proteolytic digestion showed, that the N-terminus of Dgt1p is orientated to the cytosolic side. Together with the in silicio analysis, the proteins of the LAG family have six transmembrane domains, a cytosolic C-terminus and an ER lumenal loop of …
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Some Studies on the Purification and the Properties of Monoamine Oxidase
… still associated with the membranes. The use of proteolytic digestion and DINB-Sepharose showed a similar accessibility to the enzyme from either side of the membrane. The results are discussed in terms of the role of the enzyme environment and the relationship of the enzyme protein to the outer …
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Characterisation of quiescin-sulfhydryl oxidase and nematode astacin mutants using functional studies in caenorhabditis elegans.
… modified, folded into triple helices and proteolytically cleaved at the C- and N- termini. Cross-linking of mature triple helical collagens into higher order structures leads to the generation of a flexible yet robust cuticle. Disulphide bonding is crucial in the formation of the cuticle, …
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AGEing Peptides: Synthesis and Analysis of Peptides Site-Specifically Modified by Advanced Glycation Endproducts
… forming the triple helical structure. Studies of proteolytic digestion using trypsin have revealed the dramatic effect that introduction of lysyl AGEs had on the relative enzymatic digestion rates of the host peptides. These AGEs effectively prevented trypsin from digesting the host telopeptides. …
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Molecular Level Interaction of Human Fibroblast Growth Factor-1 (hFGF-1) With Phloridzin
… like steady state fluorescence, limited trypsin digestion and protein-NMR spectroscopy. hFGF-1 needed for the study was expressed in recombinant Escherichia coli cells. The expressed protein was then purified using heparin sepharose affinity chromatography. Both expression and purification were …
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Multidimensional liquid chromatographic separations for proteomics
… monolithic reversed-phase columns is described. Proteolytic digestion of the fractions followed by rapid liquid chromatography - tandem mass spectrometry was used to complete the analysis. An alternative methodology, relying on direct analysis of the second dimension eluents by top-down (mass …
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