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Showing 1 to 20 of 43 for “"Proteinases"”.
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The roles of proteinases and proteinase inhibitors in plant-nematode interactions
… this study further confirmed the involvement of proteinases and proteinase inhibitors in plant-nematode interactions.
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Isolation and Characterization of Proteinases From the Gastric Juice of the Crayfish Orconectes Virilis (Hagen)
Made available in DSpace on 2014-12-09T23:33:51Z (GMT). No. of bitstreams: 1 6500801.pdf: 4762287 bytes, checksum: cdc7588a99969ef78d08304a85803ffe (MD5) Previous issue date: 1964
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Effects of systemic flunixin meglumine, topical oxytetracycline, and topical prednisolone acetate on tear film proteinases innormal horses
… flunixin meglumine, on the activity of two proteinases, matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9), in equine tear film. The study design consisted of twelve ophthalmically normal horses separated into three groups of four in a cross-over study design. Each …
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Characterization of the insecticidal crystal protein from Bacillus thuringiensis.
… by lepidoteran larvae. It was shown that proteinases adsorb on the surface of the crystalline body lead to proteolysis of the protein crystal especially on solubilization in alkali. Extensive washing of the protein crystal was shown to remove these proteinases and give a stable …
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Primary proteolysis of caseins in cheddar cheese
… solution was hydrolyzed by cell wall-associated proteinases from three strains of Lactococcus lactis; comparison of electrophoretograms of the hydrolyzates with those of Cheddar cheese indicated that no peptides produced by cell wall-associated proteinases were detectable in the cheeses. All the …
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Patterns of proteolysis in Danish blue, Camembert, smear-ripened and Cheddar cheeses
… specificities of Penicillium roqueforti proteinases on the caseins in milk hydrolysates were determined with a total of 91 and 118 cleavage sites identified in s1- and -casein, respectively. Proteolysis in an Irish farm-house Camembert cheese was studied during 10 weeks of ripening, …
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The role of n-linked glycosylation and sialylation in regulating human proteinase-activated receptor 1 and 4 expression and signalling
… many of the diverse biological effects of proteinases on target cells. N-linked glycosylation is a common post-translational modification in the GPCR superfamily that enables the cell to regulate protein function without recourse to the genome. Previous pharmacological studies have found …
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Isolation and characterization of cathepsin Z, a lysosomal cysteine proteinase
… E-64d, a specific inhibitor of cysteine proteinases. Cathepsin Z was not immunoprecipitated by antisera specific for cathepsins B, L, or S. Cathepsin Z has been estimated to be at millimolar concentrations in lysosomes, suggesting that it is a major lysosomal proteinase. The molecular …
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The synthesis, characterization, and use of a protein-cysteine proteinase inhibitor complex for the study of endosome/lysosome fusion
The cysteine proteinases cathepsins B, L, and S are lysosomal enzymes responsible for the degradation of endocytosed proteins. Their presence in human cell monocytic lines THP1 and U937 was detected by the use of the membrane-permeable, irreversible, active-site directed inhibitor Fmoc-(¹²⁵I)Tyr- …
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A biochemical study of some of the hydrolases to be found in the latex of the pawpaw, Carica papaya L
… the isolation and purification of three major proteinases by column adsorption chromatography; a comparative study of certain salient chemical and physical properties of these enzymes is presented. Reference is made to some conflicting results in the literature concerning papain studies, and an …
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Gene Expression and Behaviors Relating to Rotation Resistance in the Western Corn Rootworm, Diabrotica Virgifera Virgifera (Coleoptera: Chrysomelidae)
… herbivory by increasing expression of digestive proteinases is not a trait shared exclusively by rotation-resistant western corn rootworm beetles. Two proteinases, which were cathepsin B-like enzymes, exhibited consistent patterns of expression in response to inhibition of proteinase activity …
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Determining the beneficial properties of bovine amniotic membrane and its efficacy as a therapy for corneal healing
… by complex signaling pathways which involve proteinases, proteinase inhibitors, growth factors, and pro- and anti-inflammatory cytokines. For adequate corneal healing, it is necessary to have a well-balanced interaction between proteinases and their inhibitors, as well as anti-angiogenic and …
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Initial Characterization of Attachment of Infectious Pancreatic Necrosis Virus
… were adsorbed at 4°C than at 23°C or 30°C. Proteinases and neuraminidase had little effect on virus attachment. IPN virus receptors may contain alpha-D-mannosyl-like residues.
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Chemical synthesis, cloning and expression of a gene encoding systemin, a proteinase inhibitor-inducing factor
… elicit a defence mechanism by inactivating the proteinases of insects. This triggers a feedback mechanism causing overproduction of digestive enzymes together with a decrease in appetite, leading to starvation. System in, a polypeptide proteinase inhibitor-inducing factor, when applied to cut …
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Minimization of extracellular enzyme production by Pseudomonas fluorescens in a model milk system by controlled oxygen atmospheres
… 02 level which would minimize the synthesis of proteinases and lipases by P. fluorescens in a model milk system was determined in our study. Two litre volumes of Ultra-High Temperature sterilized milk (2 % m.f.) in 4 litre carboys were equilibrated with a continuous supply of atmospheric oxygen …
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Production of Extracellular Matrix-Degrading Proteases by a Rat B Cell Line.CRL-1631
… production of extracellular matrix-degrading proteinases: matrix metalloproteinases (MMPs) and components of the urokinase plasminogen activator (uP A) system. Our studies include RT-PCR of CRL-1631 eDNA revealing mRNA for MMP-2, MMP-9, TIMP-1, TIMP-2 and uPAR. MMP-2 and MMP-9 activity was …
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Analysis of the molecular interplay between Streptococcus pyogenes and its human host
… IgG4. Moreover, data suggesting that neutrophil proteinases release immunogenic epitopes from IdeS are presented. This is a novel mechanism by which S. pyogenes exploits the human immune system and prevents its virulence factors from being eliminated by opsonizing immunoglobulins.
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Valutazione dell'attività delle metalloproteinasi 2 e 9 nelle urine e nel tessuto renale di cani normali e affetti da nefropatia
Matrix metalloproteinases (MMP) are a large family of proteinases that remodel extracellular matrix (ECM) component. Recent data suggest a role for MMPs in a number of renal pathophysiologies, associated with an imbalance of ECM syntesis and degradation, which may result in an accumulation of ECM …
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Characterization of proteins and tissue remodeling components in porcine aqueous humor
… up-regulation of tissue remodeling matrix metalloproteinases (MMPs). The effects of age and gender on MMP and protein activity in porcine aqueous humor were determined in this study to identify correlations existing between MMP activity and glaucoma risk factors. Gelatin zymography identified MMPs …
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