Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"Protein-RNA interactions"”.
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Specific Bacteriophage Coat Protein-Rna Interactions
The coat proteins of the group I bacteriophages translationally repress their replicase genes by binding specifically to a region around the initiation codon called the translational operator. A large number of operator variants have been synthesized by using RNA ligase and transcription of …
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High-throughput methods to analyze protein-DNA and protein-RNA interactions
… contains the information to make thousands of RNA and protein molecules. However, a cell typically expresses only a fraction of its genes, with different phenotypes arising because of differentially expressed genes. A cell controls its gene expression at different levels. For example, it can …
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Exploring Protein -Rna Interactions With Site-Directed Mutagenesis and Phage Display
"The importance of RNA as a mediator of genetic information is widely appreciated. RNA molecules also participate in the regulation of various post-transcriptional activities, such as mRNA splicing, editing, RNA stability and transport. Their regulatory roles for these activities are highly …
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Exploring Protein-Rna Interactions With Site-Directed Mutagenesis and Phage Display
The importance of RNA as a mediator of genetic information is widely appreciated. RNA molecules also participate in the regulation of various post-transcriptional activities, such as mRNA splicing, editing, RNA stability and transport. Their regulatory roles for these activities are highly …
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Exploring protein-RNA interactions with site-directed mutagenesis and phage display
The importance of RNA as a mediator of genetic information is widely appreciated. RNA molecules also participate in the regulation of various post-transcriptional activities, such as mRNA splicing, editing, RNA stability and transport. Their regulatory roles for these activities are highly …
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Study Of Protein-Rna Interactions Using Fluorescence Resonance Energy Transfer (fret) And Single-Molecule Fret
<p>In the cell, RNA and protein, interact to form ribonucleoprotein complexes (RNPs) that have vital structural, catalytic and regulatory roles. Despite their functional importance, the mechanistic details and dynamics of RNPs are poorly understood. Single-molecule Fluorescence Resonance Energy …
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Kinetics and thermodynamics of protein-RNA interactions and protein folding in vitro and in cells
This Dissertation was approved for publication on 2015-04-20 at 07:49.
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Protein-Protein and Protein-Rna Interactions in the in Vitro Assembly of the R17 Bacteriophage
… R17 bacteriophage is a positive single stranded RNA virus that specifically infects male strains of E. coli. The role of RNA protein interactions in the in vitro assembly of the virus is examined in order to determine both what features of the RNA are important for its selective packaging by R17 …
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Engineered mRNA regulation using an inducible protein-RNA aptamer interaction
… of naturally occurring regulation of RNA function in biology is increasingly being recognized. A common regulatory mechanism uses inducible protein-RNA interactions to shape diverse aspects of cellular RNA fate. Recapitulating this using a novel set of protein-RNA interactions is …
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DEVELOPMENT OF VIRTUAL SCREENING METHODOLOGIES FOR IDENTIFYING RNA-BASED THERAPIES
… of computational methodologies for predicting protein-RNA binding affinities and designing RNA sequences with specific functional outcomes. The research introduces PANTHER (Protein-Affinity for Nucleic Target-binding, Hybridization, and Energy Regression), a novel machine learning-based scoring …
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Transcriptomics-based study reveals a novel link between arginine methylation and phosphate regulation in Saccharomyces cerevisiae
… regulation, nucleocytoplasmic transport of proteins and mRNAs and RNA splicing. Hmt1 is also known to mediate protein-protein and protein-RNA interactions. Despite its well-studied role in regulatory processes, the effects of Hmt1 knockout on the transcriptome has never been performed. This …
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Investigating the Role of Muscleblind-Like 1 in the Suppression of Breast Cancer Progression
… role in modulating transcriptional output. The interactions between messenger RNA cis-regulatory elements and trans-factors control coordinated gene expression states. Posttranscriptional regulatory programs that enhance metastatic capacity are selected for during cancer progression. In this …
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The Role of Secondary and Tertiary Structure in the Cap-Independent Translation of FGF-9 and HIF-1-alpha
… conditions, eukaryotes initiate translation of RNA through eIF4E recognition of the 5’ cap. However, under cellular stress, eukaryotic translation must be initiated through a 4E-independent, or “cap-independent” mechanism, involving eukaryotic initiation factor 4G (eIF4G) binding directly to the …
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Structure determination of a yeast ribosomal protein L30 and pre-mRNA binding site complex by NMR spectroscopy
The yeast (Saccharomyces cerevisiae) ribosomal protein L30 and its auto-regulatory pre-mRNA binding site provide one of the best examples the critical role of protein-RNA interactions in regulation of RNA processing and control of gene translation. A model system for this interaction, which …
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Engineering control of eukaryotic translation with application to the malaria parasite Plasmodium falciparum
… need, we engineered a new system for control of protein translation in eukarvotes, and applied it to P. falciparum. This system is based on the ligand-regulated interaction between an RNA aptamers and the TetR-repressor protein. Although such protein-RNA interactions are abundant in nature and …
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TDP-43 and its role in neurodegeneration
Ubiquitinated TDP-43 (TAR DNA Binding Protein) inclusions are a hallmark of ALS (amyotrophic lateral sclerosis) and FTLD-TDP-43 (frontotemporal lobar degeneration with ubiquitin inclusions). These diseases share a similar pathology of cytoplasmic ubiquitinated TDP-43 inclusions, which contain …
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RNA Dynamics in T Cell Activation
… tune gene expression programs in response to external signals. Regulation of messenger RNA and microRNA by RNA-binding proteins is critical to the control of this process. Hence, disruptions of RNA regulatory processes result in neurologic diseases, cancer, and immunologic disorders among other …
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Structure determination of bacteriophage [phi]21 N-peptide and boxB RNA complex by NMR spectrocsopy
The antitermination protein N from [delta] and related E. coli bacteriophage interacts with the nut RNA site and host factors to transform RNA polymerase (RNAP) into a termination resistant transcription complex. The [delta], P22 and [phi]21 phage N proteins share a conserved amino-terminal, …
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Discovering non-equilibrium mechanisms that regulatestructure and function of biomolecular condensates usingphase-field modeling
… in living cells that form through cooperative interactions between their constituents such as proteins and RNAs. They are emerging as important organizers of biochemistry in cells and are dysregulated in disease. Understanding the physical principles that shape the form and function of these …
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Co-transcriptional recruitment of the U1 snRNP
… believed that the splicing of most pre-mRNAs occurs, at least in part, co-transcriptionally. In order to validate this principle in yeast and establish an experimental system for monitoring spliceosome assembly in vivo, I have employed the chromatin immunoprecipitation (ChIP) assay to …
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