Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"Protein-RNA interaction"”.
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Engineered mRNA regulation using an inducible protein-RNA aptamer interaction
… of naturally occurring regulation of RNA function in biology is increasingly being recognized. A common regulatory mechanism uses inducible protein-RNA interactions to shape diverse aspects of cellular RNA fate. Recapitulating this using a novel set of protein-RNA interactions is …
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Casting Protein Structure Predictors as Energy-Based Models for Binder Design and Scoring
Protein binder design has been transformed by hallucination-based methods that optimize structure prediction confidence metrics, such as the interface predicted TM-score (ipTM), via backpropagation. However, these metrics are imperfect proxies for binding affinity and do not reflect the statistical …
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Single molecule pull down of double strand RNA binding proteins
RNA molecules are transcribed as single stranded naturally, but with most of them forming in to structures composed of duplex regions, loop, bulge or mismatches. RNAs with double stranded regions, or known as double-strand RNAs (dsRNAs). The class of proteins responsible for processing dsRNAs is …
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Biochemical Analysis of Evolutionary Divergent Vertebrate Larp6 Proteins
The La-related proteins (LARPs) contain a conserved bipartite RNA binding domain, called the La module, which consists of the La motif and an RNA recognition motif (RRM). Across the LARP subfamilies, the La module interacts with a variety of RNAs, allowing this protein superfamily to perform roles …
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Structure determination of a yeast ribosomal protein L30 and pre-mRNA binding site complex by NMR spectroscopy
The yeast (Saccharomyces cerevisiae) ribosomal protein L30 and its auto-regulatory pre-mRNA binding site provide one of the best examples the critical role of protein-RNA interactions in regulation of RNA processing and control of gene translation. A model system for this interaction, which …
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Computational Modeling of Rna-Small Molecule and Rna-Protein Interactions
<p>The past decade has witnessed an era of RNA biology; despite the considerable discoveries nowadays, challenges still remain when one aims to screen RNA-interacting small molecule or RNA-interacting protein. These challenges imply an immediate need for cost-efficient while predictive …
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The Interaction Between Eukaryotic Translation Initiation Factor eIF4G and 3’ Cap Independent Translation Element of Barley Yellow Dwarf Virus Is Affected by Multiple Initiation Factors
… in the 3’ untranslated region of the viral mRNA. BTE (Barley Yellow Dwarf Virus like cap-independent translation element) is one of the well characterized CITEs. BTE mediated translation primarily depends on eukaryotic initiation factor eIF4G. BTE binds to eIF4G; however, the details of BTE …
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Unraveling the interactions between MBNL1 protein and its RNA targets
The muscleblind-like protein 1 (MBNL1) is an RNA binding protein with four conserved zinc fingers that plays an important role in the regulation of alterative splicing. It interacts with pre-mRNA and promotes the inclusion or exclusion of exons to form different mRNA transcripts. Besides its …
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Investigation of the interactions in RNA recognition motif-RNA complexes: small molecule inhibitors and kinetics of dissociation
The RNA recognition motif (RRM) is the most abundant RNA binding domain that is found in all organisms. RRM-containing proteins participate in most steps of gene expression, including translation, splicing, modification and transport of RNA. This dissertation aims to help understand the …
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Apobec-1 Complementation Factor (ACF) Binds and Regulates Multiple RNAs
AU-Rich Elements: ARE) are cis-acting RNA sequences in the 3'UTRs of a wide range of transcripts that function to regulate mRNA stability, localization, and/or translation through interaction with ARE-Binding Proteins. Apobec-1 Complementation Factor: ACF) was originally identified as a co-factor …