Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"Protein stabilization"”.
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Elucidating protein stabilization with fluorescence techniques
Insulin is an important protein that controls blood glucose levels, but its function becomes ineffective during protein aggregation. Insulin readily undergoes protein aggregation and it contributes to hyperglycaemia. In long-term hyperglycaemia, glucose can alter the structure and stability of …
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Optogenetic regulation of protein activity in live cell
… phenotypes depending on the dynamic profile of protein activity. Conventional genetic and pharmacological approaches such as gene overexpression, use of growth factors or inhibitors have helped us delineate interaction maps of signaling components, however these techniques provide limited means …
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The identification of the mTOR-regulated phosphoproteome and a mediator of feedback inhibition to P13K-Akt
The mTOR protein kinase nucleates two complexes, mTORC1 and mTORC2. Collectively, the two complexes regulate processes important for cell growth and proliferation, including protein synthesis, autophagy, metabolism, and cytoskeletal maintenance. Despite this diverse array of cellular functions, few …
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THE ROLE OF THE TRANSCRIPTION FACTOR DEAF-1 IN PROSTATE FUNCTION
… and hydrogen peroxide. Increase in DEAF-1 protein levels could be due to post transcriptional mechanisms leading to protein stabilization. DEAF-1 over expression in PC3 cell lines leads to a increase in G2/M arrest suggesting altered levels of DEAF-1 in cancer may lead to disruption of …
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RAR beta trans-repression of AP-1 transcription factor in HeLa cervical cancer cells : consequences on transcription of viral and cellular AP-1 controlled genes
… the phosphorylation of c-Jun is important for protein stabilization, HeLa RARß2 clones were treated with different c-Jun-N-terminal kinase (JNK) stimulators. The treatments resulted in a c-Jun increase at RNA and protein levels, and led to a reconstitution of AP-1 binding similar to …
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Computational Studies of Bio-Inspired Synthetic Random Heteropolymers
Biological heteropolymers, such as proteins and nucleic acids, work together to execute the vast suite of tasks which ultimately enable life. These capabilities, particularly enzymatic function, are incredibly attractive for developing sustainable materials, removing pollutants and contaminants, …
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Protection Mechanisms of Excipients on Lactate Dehydrogenase during Freeze-Thawing and Lyophilization
… and mechanisms of excipients on a labile model protein, lactate dehydrogenase (LDH), during freeze-thawing and lyophilization. Cryoprotectants are excipients that protect labile molecules during freezing. Some investigators believe that preferential exclusion is the predominant mechanism to …
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ATR SIGNALING IN GENOME REPLICATION AND MAINTENANCE
… transcription control and may also involve protein stabilization of specific HR factors. Interestingly, cancer cells seem to exhibit a stronger dependency on ATR signaling for maintaining the levels of HR factors, and I propose that elevated constitutive ATR signaling caused by augmented …
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p53 Functional Interactions: the Study of a New Crosstalk with Estradiol Pathway in Transcriptional Responses to Chemotherapeutics
… factor p53, a critical tumor suppressor protein, specifically, the crosstalk (or functional interactions) with other transcription factors, namely, the estrogen receptors, and the modeling in reconstituted assays of the interaction of p53 with positive and negative cofactors (e.g. MDM4 …
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Mechanisms Regulating Pulmonary Sensitivity to Radiation
… by quantitative real-time PCR and their protein products validated by western blot. Finally, we performed longitudinal analysis of hypoxia-associated gene expression to elucidate the natural history of disease progression in "fibrosis prone" C57BL/6J mice. </p><p>In these studies, we …
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Insulin and TOR pathways regulate cellular and organismal growth through the myc oncogene in Drosophila
… at the mRNA and potein level. We believe dMyc protein accumulation after Insulin signaling activation is conditioned to AKT-dependent GSK3β/sgg inactivation. In fact, we were able to demonstate that dMyc protein stabilization through phosphorylation is a conserved feature between Drosophila and …
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Discovery of Inhibitors of Inhibitors of Apoptosis Proteins and Novel Tubulin Polymerization Inhibitors as Potential Anticancer Agents
… Survivin, a member of the inhibitor of apoptosis proteins (IAPs) family, serves as both an apoptosis suppressor and a pivotal player in cell division. Its significance as a cancer drug target arises from its heightened expression in various human cancers, juxtaposed with its minimal presence in …
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Studies into the receptor-mediated induction of hypoxia inducible factor 1α.
… and facilitates dramatic up-regulation of the protein stability and transcriptional activation capabilities of the HIF a-subunits. HIF also appears to play a role in target gene transcription in a hypoxia-independent manner. This novel, hypoxia-independent function of HIF is poorly …
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Mechanistic approaches towards understanding particle formation in biopharmaceutical formations. The role of sufactant type and level on protein conformational stability, as assessed by calorimetry, and on protein size stability as assessed by dynamic light scattering, micro flow imaging and HIAC
Control and analysis of protein aggregation is an increasing challenge to biopharmaceutical research and development. Therefore it is important to understand the interactions, causes and analysis of particles in order to control protein aggregation to enable successful biopharmaceutical …
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Management strategy of landfill leachate and landfill gas condensate
… species in landfill leachates under various stabilization states were investigated. Although the effect of landfill stabilization state on the characteristics of organic matter and ammonia is well documented, there are few investigations into the landfill leachate organic nitrogen under …