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Showing 1 to 20 of 161 for “"Protein stability"”.
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Roles of cosolvents on protein stability
The function of a protein is determined by its three-dimensional structure which emerges from the delicate balance of forces involving atoms of the protein and the solvent. This balance can be perturbed by changing temperature, pressure, pH and by adding organic molecules known as cosolvents to the …
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The Effect of HACE1 on RAR Protein Stability
… domain of RARs may be regulated by interacting proteins such as HACE1. In vitro and in vivo studies in our lab have shown that HACE1 interacts with RARα1, - β1, -β2, -β3, and -γ1 at the variable AF-1 domain. Transactivation studies have shown that HACE1 represses RA dependent transcriptional …
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Development and Characterization of a Novel Protein Stability Probe
… has been well-characterized as regulating MYC stability. Current strategies to measure protein half-life are low-throughput or prone to false readouts, and have hampered the discovery of novel regulatory pathways for MYC that could be targeted therapeutically. To overcome this barrier, we …
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Studies of Protein Stability by Hydrogen Exchange-Mass Spectrometry
With the rapid growth in using proteins as therapeutics, studying the stability of therapeutic proteins has become an essential part in the manufacturing of therapeutics to ensure the quality, safety, and efficacy of a drug product. Proteins can get exposed to various stresses and undergo various …
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Regulation mechanisms of Drosophila retinoblastoma protein stability and repression activity
The retinoblastoma (RB) family proteins are pivotal transcriptional corepressors involved in diverse cellular events. As a potent tumor suppressor, RB plays a variety of important roles including regulating cell cycle, promoting cellular differentiation, inducing cell senescence and balancing …
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Copper as an Antibacterial Agent and Disruptor of Protein Stability
… metal-shuttling ionophores. Next, proteome-wide protein expression level and stability measurements were used to probe treatment-induced cellular changes after E. coli were exposed to Cu in the absence and presence of PT or PcephPT. The stability-based study identified key protein targets such as …
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A study on human IFIT3 modulation of IFIT1 protein stability
Interferon-induced proteins with tetratricopeptide repeats (IFITs) are expressed in both interferon-dependent and interferon-independent manners in cells after pathogen-associated molecular pattern recognition by the pathogen recognition receptors. Four IFITs (IFIT1, IFIT2, IFIT3 and IFIT5) have …
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Deubiquitinating Enzymes Promote Cancer Progression and Metastasis Via Regulating Protein Stability
… or polyubiquitin chains from target proteins. DUBs have critical roles in cell homeostasis and signal transduction, as they regulate protein degradation, subcellular localization, and protein-protein interaction. Deregulation of DUBs contributes substantially to tumor formation and …
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Protein stability: Impact of formulation excipients and manufacturing processes in protein-based pharmaceuticals
Presently, over 300 proteins or peptide based therapeutic medicines have been approved by the FDA owing to advances in protein engineering and technology. However, majority of these protein-based medications are unstable or have limited shelf life when in aqueous form. During pre-formulation and …
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Development of a single-cell fluorescence-based sensor to investigate Myc protein stability
… to cancer have made Myc a thoroughly studied protein and an extremely desirable drug target, over fifty years of research have failed to produce an inhibitor that has successfully reached the clinic. Drug design against Myc has been limited by its lack of a defined structure with exploitable …
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A study of novel tools to measure and perturb Aurora Kinase A protein stability
… classes in biology, we have been taught that proteins are the ‘molecular machinery’ of the cell, and that they carry out the cellular functions within the cell. At a top-down level, the amount of proteins present within a cell is a balance between its synthesis and degradation, the dynamic …
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Comparing Solution-Phase and Gas-Phase Protein Stability Using Ion Mobility and Differential Mobility Mass Spectrometry
A critical quality attribute for many proteins concerning biopharmaceutical products is the proteins stability. Current stability assays are lengthy but some quick stability assays to determine the proteins melting temperature include thermal ramps of a protein using a fluorescent dye to monitor …
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Role of Sub-Cellular Localization and Cellular Factors In Regulation of HIV-1 Tat Protein Stability
<p>Human Immunodeficiency Virus (HIV) -1 Tat protein plays an indispensable role in viral gene transcription. Tat is reported to produce a favorable environment for viral replication by modulating a variety of cellular processes. A vast amount of literature is available on the function and effects …
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Advancing fast relaxation imaging to determine protein stability and folding kinetics on poly(n-isopropyl acrylamide) films
… polymer PNIPAM to capture and release cells or proteins for medical purposes; however, this assumes that PNIPAM does not perturb the stability of biomolecules. Previous analytical and spectroscopic techniques have not been able to confirm this assumption due to limitations in detection of …
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Synthesis of doxorubicin-albumin conjugates via cobalt coordination chemistry: The effect of reaction conditions on overall protein stability
… while increasing therapeutic effect. Protein-drug conjugates are a rapidly expanding family of therapeutics that hold potential for ameliorating off-target toxic effects observed in chemotherapy. In this context, human serum albumin could act as a nanoscale delivery vector to alter the …
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