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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"Protein self-assembly"”.
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Engineering protein self-assembly for biosensing applications
Protein self-assembly is a promising bottom-up approach to create biological materials with programmable functionalities, particularly for biotechnological applications. This approach leverages engineering recombinant protein building blocks, derived from natural or artificially designed proteins, …
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Molecular Mechanisms of Protein Self-Assembly and Aggregation
… we investigate the mechanisms driving the self-assembly of peptides and proteins using computational and theoretical tools, always validating our results with experimental measures when possible. In the first part, Chapters 2-5, we focus on the A$\beta$ system, a peptide whose aggregation …
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Methods for study and manipulation of protein self-assembly
The self-assembly of soluble proteins into amyloid aggregates is a mechanism which can be observed in processes that are functional or disease-associated. The resulting fibrillar structures can be formed from a variety of different proteins, but all share a β-sheet rich architecture and a variety …
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Advances in biophysical characterisation through micron scale flow engineering
Proteins are the chief actor molecules of cells central to the majority of biochemical and biophysical processes that sustain life. Interactions between proteins and other biomolecules are crucial to a faultless execution of biological function, yet it has remained challenging to analyse these …
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Microfluidic formation of hierarchical micro and nano emulsions for biomedical applications
The self-assembly of proteins and peptides into complex supramolecular structures provides an important avenue in the development of materials for biomedical applications. In particular, the design and fabrication of protein and peptide-based hydrogels has emerged as an attractive route toward …
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Probing biomolecular reactions and self-assembly in microfluidic systems for quantitative measurements and therapeutic applications
… is used to enable quantitative measurements of proteins and other biomolecules. In addition, microfluidics is used to create otherwise inaccessible structures, designed to modulate biological systems in a controlled way. More specifically, this thesis studies the effect of shear in the …
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Amyloid β Aggregation is Coupled to Cellular Metabolism and Intramitochondrial Proteostasis
… However, little is known about the dynamics of protein self-assembly, and effective inhibitors have not yet been identified. This lack of success can be associated with limitations affecting the standard techniques employed to study peptide self-assembly. Drawbacks include the inability to …
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Peptide Self-Assembly from the Molecular to the Macroscopic Scale at Standard Conditions
… to address the problem of how to prepare protein-based materials with the same level of order and precision at the molecular level similar to the structures we find in nature. It is divided into two parts focusing on feedstock and processing. Part one is devoted to discussing the use of …
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Microfluidics and chemical kinetics to analyse protein interactions, aggregation, and physicochemical properties
Proteins play a major role in living systems and present a wide spectrum of functionalities. Many different types of proteins are involved into biological processes, such as the catalysis of biochemical reactions, cellular membrane transport, immune system response and DNA replication. However, …
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Molecular structure, hierarchical assembly and stimuli-responsive mechanics of tropoelastin and elastin biomaterials
… associated with mutations therein, and assembly process, to replicate natural function upon impairment. From the materials perspective, elastin-based materials display tunable thermal sensitivity, presenting opportunities to mimic and control these responsive features for biomedical …
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Controlled self-assembly of natural proteins into hierarchically structured functional materials
Protein self-assembly offers key strategies for nature to generate high-performance and multifunctional proteinaceous materials. Inspired by nature’s approach, there is growing interest in the exploitation of self-assembled protein structures as a basis of artificial protein materials. To this end, …
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Hierarchical protein assemblies with biomedical applications
Molecular self-assembly is a key process evolved by nature, through which functional materials are generated. Among these processes, protein self-assembly into hierarchical structures provides an important avenue for the development of materials with a wide range of biomedical applications. …
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Design of Collagen-Mimetic Peptides
… core of the diverse functions of this versatile protein is its remarkable ability to form supramolecular structures through self-assembly and/or through interactions with other biomolecules. One typical example of such supramolecular structure is the fibrils of collagen types I, II and III of the …
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Self-Assembly of Large Amyloid Fibers
… adder' cooperative mechanism for the spontaneous self-assembly of micrometer sized amyloid fibers. A short hydrophobic template peptide induces a conformation change within a highly α-helical adder protein to form β-sheets that continue to assemble into micrometer sized amyloid fibers. This study …
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Development of Polymer-Biomolecule Core-Shell Particles For Biomedical Applications
… chemotherapy. The vehicle was created by the self-assembly of folate-grafted filamentous bacteriophage M13 with poly(caprolactone-b-2-vinylpyridine) while doxorubicin, the antitumor drugs, was successfully loaded in the interior of the vehicles. These particles offer unique properties of being …