Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 84 for “"Protein secretion"”.
-
Molecular Mechanisms of SURF4- mediated Protein Secretion
Protein secretion is an essential process that drives organelle biogenesis, cell growth and communication. Once synthesised and processed in the Endoplasmic Reticulum (ER), secretory proteins are incorporated into transport carriers that are generated by the COPII coat. Efficient and accurate cargo …
-
Investigation of conventional and unconventional protein secretion using galectin-3
Protein secretion is an essential process in cell biology. The conventional secretion pathway is well known, and involves proteins with a signal sequence being directed into the endoplasmic reticulum (ER). Once in the lumen of the ER, proteins are trafficked through the Golgi and remain separate …
-
Adiporedoxin, an upstream modulator of endoplasmic reticulum oxidative folding and protein secretion
… adipocytes Adrx knock down decreased the secretion of numerous adipokines, extracellular matrix, and transmembrane proteins and over expression increased secretion. Together, these results suggest Adrx regulates an early step in protein secretion from the ER. Immunofluorescence and …
-
Characterisation of the putative Type VII protein secretion system of <em>Streptomyces</em> spp
… genes encoding components of putative Type VII protein secretion systems (T7SSs). To investigate the role of these putative T7SSs in S. coelicolor and S. scabies, marked deletions were constructed in genes encoding core components of the machinery. Phenotypic analysis revealed that deletion of …
-
The effects of cis- and trans-acting mutations on recombinant protein secretion in Pichia pastoris
… research and industrial settings for recombinant protein expression due to the ease of genetically modifying its genome, its ability to grow to large densities in inexpensive media, and its capability to perform posttranslational modifications. Multiple tools such as the cis -acting factors MATα …
-
Regulation of Phospholipid Transfer Protein Secretion and Gene Expression in the Hepatoma Cell Line, HepG2
Hep G2 cells were used as a model to study HDL distribution and interconversion corresponding to variations in PLTP activity. An increase in PLTP activity by butyrate treatment was accompanied by increases in the amount of HDL having a hydrated density of 1.10 g/ml, HDL particle size and the …
-
Increasing Heterologous Protein Secretion From the Yeast S. Cerevisiae Through Manipulation of Cellular Redox Factors
… the effect of cellular redox factors on BPTI secretion. Glutathione levels and redox state were altered in an attempt to determine if optimal BPTI secretion requires a certain total glutathione level and/or redox state. Evidence for an optimal glutathione level that results in high BPTI …
-
Characterization of protein secretion in Mycobacterium leprae using phoA fusions in Escherichia coli and Mycobacterium smegmatis
… genome has provided new information related to proteins constituting its hypothetical proteome. Since M. leprae can not be grown in vitro, novel approaches are needed to determine which proteins are expressed during infection and whether these proteins are related to pathogenesis. Secreted …
-
In vitro milk protein secretion by explants of Holstein bull mammary tissue from two different genetic lines
… in organ culture to synthesize and secrete milk proteins was studied. Sixteen bulls from the genetic selection and control lines developed at Virginia Polyteclmic Institute were randomly assigned to either a non-treated or steroid pretreated group. The steroid pretreated group received estradiol …
-
Effect of laminar shear stress on gene regulation, protein synthesis, and protein secretion by cultured human endothelial cells
… cell expression of blood clot dissolving proteins or vasoactive proteins, an in vitro perfusion system was used to expose human umbilical vein endothelial cell monolayers to well defined, laminar fluid flow. Protein production studies utilized immunoassays, while semi-quantitative studies …
-
The structural characterization of the Saccharomyces cerevisiae alpha mating factor secretion signal for recombinant protein secretion in Pichia pastoris
… been used extensively for expressing recombinant proteins because it combines the ease of genetic manipulation with rapid growth to high cell densities and provides complex posttranslational modifications. The most successful and commonly used secretion signal leader in Pichia pastoris has been …
-
Investigating the role of TspA, a novel substrate of the <i>Staphylococcus aureus</i> Type VII protein secretion system
… and hospital-acquired infections. The Type VII Secretion System (T7SS) is widespread in Gram-positive bacteria belonging to both the Actinobacteria and Firmicutes phyla. In commonly-studied strains of <i>S. aureus</i> the T7SS gene cluster is encoded by 12 genes, and it has been shown to play an …
-
Protein-Protein Interaction and Structure-Function Studies of XcpR, a Protein Involved in Extracellular Protein Secretion of Pseudomonas Aeruginosa
… hydrolytic enzymes and toxins. The extracellular secretion of these proteins requires at least eleven xcp gene products (XcpP-Z) and the bifunctional peptidase/methylase, PilD. Although a number of macromolecular translocation mechanisms have been found in a wide range of bacterial species that …
-
A Mutational Analysis of XcpQ, the Outer Membrane Component of the Type II Protein Secretion System From Pseudomonas Aeruginosa
… LipC cryptic lipase are exported via a Type II secretion apparatus. XcpQ is the sole outer membrane protein component of this system and is a member of the superfamily of proteins called secretins. XcpQ forms extremely stable pore-like outer membrane multimers, through which Type II secreted …
-
Characterization of the type VI protein secretion system encoded in the Salmonella pathogenicity island 19 and its role in the pathogenicity of serotypes Gallinarum and Enteritidis
El genero Salmonella comprende a mas 2,500 serotipos conocidos distribuidos en dos especies: enterica y bongori. Estos serotipos difieren mucho en términos de patogenicidad y especificidad hospedero. Dos serotipos de Salmonella entérica son de especial relevancia: los serotipos Gallinarum y …
-
A directed evolution approach to engineering recombinant protein production in S. cerevisiae
The continued success of protein therapeutics has put a strain on industry's ability to meet the large demand. Creating a more productive expression host for the manufacture of these proteins is a potential solution. Although heterologous proteins are frequently made in organisms as disparate as E. …
-
Selenium Effects on the Trabecular Meshwork
… matrix turnover, I examined alterations in protein secretion and intracellular signaling. MSeA treatment (5-10 uM) led to a significant decrease in the secretion of matrix metalloproteinase -2 and its inhibitor after 6-24 hours and to a dose-dependent decrease in kinase signaling. Later, I …
-
IMPROVING GLYCOSYLATION EFFICIENCY IN ESCHERICHIA COLI BY MAMMALIAN-CELL-INSPIRED PROTEIN-PROTEIN INTERACTIONS
… common post-translational modifications found in proteins. The central enzyme in the N-glycosylation pathway responsible for the transfer of glycans onto asparagine residues is called the oligosaccharyltransferase (OST). In mammalian cells, most proteins are N-glycosylated cotranslationally, …
-
Characterizing phenotypes of Pichia pastoris mutants that show enhanced secretion of recombinant proteins
… and isolate genes that are associated with protein secretion, the Lin-Cereghino laboratory at University of the Pacific created mutant strains of Pichia pastoris using the restriction enzyme mediated integration method. The mutants exhibited an unusual ability to supersecrete …
Page 1 of 5