Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 83 for “"Protein purification"”.
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PROTEIN PURIFICATION FROM GASTROPODS
… of their tissues by using lectins as glycoprotein-specific antibodies. Purification work started with the screening of 7 different exoglycosidases (α-fucosidase, β-xylosidase, α-mannosidase, β-mannosidase, α-glucosidase, β-glucosidase, β- galactosidase and β-N-acetylglucosaminidase) in 8 …
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Novel internally-staged ultrafiltration for protein purification
… stack has been developed to fractionate proteins closer in molecular weight than conventionally possible. The technique is illustrated here by obtaining a pure protein product from a binary protein mixture. By employing membranes in series using the same membrane without any gaskets or …
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Delipidation Treatments for Large-Scale Protein Purification Processing
… insoluble. Lipid removal is desired prior to protein purification processing to decrease nonspecific fouling of downstream chromatographic matrices. Transgenic pig milk was used as a model system to study delipidation from therapeutic protein sources. The majority of triglycerides was …
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Establishment of enhanced protein purification strategies for recombinant immunotherapeutics
… protocol to produce high quality recombinant proteins play a pivotal role in supporting both lab-scale research and pharmaceutical applications. Although relying on the limited availability of resources, the MB&I research unit successfully employs a single IMAC step to purify mammalian and …
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Particle assisted protein crystal nucleation as a protein purification platform for pharmaceutical manufacturing
Slow protein crystal nucleation is a major barrier to using crystallization as a separation and purification strategy in protein drug manufacturing. This work investigates the use of functionalized nanoparticles in solution to act as in situ templates for initiating crystal nucleation. We used …
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Protein purification and cDNA cloning of suGF1 : a sea urchin nuclear DNA-binding factor
… over this area. A poly( dG).poly( de)-binding protein (suGF1) has previously been identified and purified on a small scale from embryonic nuclear extracts of the sea urchin Parechinus angulosus (1, 2). suGF1 binds in vitro to the H1-H4 intergenic region of the early histone gene battery, and …
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R-RAS41A fusion protein purification and the cloning of a new RAS GEF like gene
This document only includes an excerpt of the corresponding thesis or dissertation. To request a digital scan of the full text, please contact the Ruth Lilly Medical Library's Interlibrary Loan Department (rlmlill@iu.edu).
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Experimental studies of RctB- initiator of Vibrio cholerae chromosome II replication: genetic engineering, protein purification, and protein crystals
… replication is controlled by RctB, a DNA-binding protein that is an exclusive attribute of all Vibrio species. RctB has no homologs among other members of bacteria proteins and can serve as a target for designs of Vibrio-specific antibacterial medications. Elucidation of the molecular structure of …
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Characterization of a Bromoperoxidase From the Marine Algae Penicillus Capitatus
… oxiations while in the presence of peroxide. Protein purification and characterization of this enzyme showed that the prosthetic group is ferric protoporphyrin IX and that the enzyme consists of two equal molecular weight subunits whose combined weight is approximately 100,000. Bromoperoxidase …
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Regulation of the Tubulin Homolog FtsZ in <i>Escherichia coli</i>
… all fields of biological sciences for cloning, protein purification, and as a model for Gram-negative bacterial species. And yet, researchers do not fully understand how this bacterium replicates and divides. Every year additional division proteins are discovered, which adds complexity to how we …
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Therapeutics discovery via glycotechnology : the development of protein engineered enzymes for the study of galactosaminoglycan neuromedicine
… cloning, recombinant genetic expression, and protein purification of glycosaminoglycan-degrading enzymes. To this end, enzymes from the chondroitinase family of Pedobacter heparinus and Proteus vulgaris were developed. This library of enzymatic tools will reveal glycoconjugate …
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High-Throughput Detection of Protein---Protein and Protein---Dna Interactions via Photonic Crystal Biosensors: Applications to Apoptosis Inducing Factor
… Macromolecular interactions including protein---small molecule, protein---nucleic acid, protein---protein, and whole cell interactions can be measured using PC biosensors. Presented herein is the first application of PC biosensors to a protein---nucleic acid interaction and a simplified …
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High-throughput detection of protein—protein and protein—DNA interactions via photonic crystal biosensors: applications to apoptosis inducing factor
… Macromolecular interactions including protein—small molecule, protein—nucleic acid, protein—protein, and whole cell interactions can be measured using PC biosensors. Presented herein is the first application of PC biosensors to a protein—nucleic acid interaction and a simplified …
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Self-organization of Microtubule and Associated Protein Map65
… of biophysics. In this thesis, via a minimal two-protein in vitro system containing cytoskeletal fiber microtubule and associated protein MAP65, I try to address self-organization through passive entropic force mechanisms. I use techniques like protein purification, light microscopy, and image …
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Copper and rheumatoid arthritis - an in vitro study of metal-protein interactions
… apparatus to study metal interactions with blood proteins. The results are compared with equilibrium dialysis. The systems studied using this technique includes, the binding of Cu(ll) to HSA and BSA, ternary Cu(ll)-HSA-amino acid systems, as well as competitive studies of the Cu(ll)-HSA system …
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Characterization of Type IV Pilus System Genes and Their Regulation in Clostridium perfringens
… also provided data to develop a method for protein purification from C. perfringens without induction.
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Identification of Critical Residues in Spliceosomal Protein Dib1
… must occur before translation into functional protein occurs. One important processing event is known as splicing. Splicing is the removal of non-protein coding regions, known as introns, from pre-messenger RNA and the ligation of the protein coding regions, known as exons, to form a mature …
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ENGINEERING WATER-SOLUBLE VARIANTS OF THE SINGLE-SUBUNIT OLIGOSACCHARYLTRANSFERASE
… a key enzyme in the asparagine-linked (N-linked) protein glycosylation pathway. OSTs exist in all domains of life and are capable of transferring a preassembled glycan from lipid carrier to an acceptor peptide. Bacterial OSTs are an single-subunit enzyme that are amenable to recombinant expression …
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Manufacturing of agarose-based chromatographic adsorbents with controlled pore and particle size
… performed using stationary phases designed for protein purification, typically employing pore sizes of about 40 nm. This results into a severe underexploitation of the porous structure of the adsorbent as adsorption of plasmid DNA occurs almost exclusively on the outer surface of the adsorbent. …
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Characterization of Novel Type VI Effectors of Acidovorax citrulli and Their Applicability to Biological Control of Plant Diseases
… A group of novel non-enzymatic effectors, Hyde1 proteins, delivered into prey cells through the T6SS, are responsible for this broad-spectrum antimicrobial activity. Expressing Hyde1 or its N-terminal transmembrane domain shows significant toxicity in both E. coli and AAC00-1, and the toxicity of …
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