Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 41 for “"Protein profiling"”.
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Synaptic Protein Profiling in the Mammalian Brain
… through the precise localization of synaptic proteins, yet it has not previously been possible to analyze the protein content of an individual class of synapses. In order to achieve this, we have used the BAC (bacterial artificial chromosome) transgenic approach to target particular neurons …
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Gene and Protein Profiling of the Preeclamptic Placenta
… to screen and profile the placenta, gene and protein expression, for changes related to preeclampsia (PE) and cases with increased resistance in the uterine arteries. Women with increased resistance in the uterine arteries have increased risk of developing PE. Since not all of them develop PE, …
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Kinetic Characterization, Inhibition, and Activity-Based Protein Profiling of Protein Arginine Methyltransferase 1
<p>Protein arginine methyltransferases (PRMTs) are SAM-dependent enzymes that catalyze the mono- and di-methylation of peptidyl arginine residues. Although all PRMTs produce mono-methyl arginine (MMA), type I PRMTs go on to form asymmetrically dimethylated arginine (ADMA), while type II enzymes …
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Mass spectrometry-based protein profiling strategies for biomarker discovery in liver and inflammatory bowel diseases
The study of protein expression profiles for biomarker discovery in serum and in mammalian cell populations needs the continuous improvement and combination of proteins/peptides separation techniques, mass spectrometry, statistical and bioinformatic approaches. In this thesis work two different …
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Activity-Based Protein Profiling Reveals Changes to the Regulation of Enzymatic Activity by the Hepatitis C Virus
… to specific amino acids. Activity-based protein profiling allows the potential for activity of target enzymes to be measured, thereby gaining a more accurate representation of the functional state of the biological system. In this work, profiling differential enzyme activity allows the …
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Synthesis of activity-based protein profiling probes for malaria and hypertension disease models & potential novel ACE inhibitors with an attenuated zinc binding group
Angiotensin-converting enzyme (ACE) and P. falciparum A M1 (PfA-M1) are both zinc metalloproteases implicated in hypertension and malaria, respectively. Hypertension affects approximately 26 % of the world’s population while each year over 300 million cases of malaria occur worldwide resulting in …
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Design and Synthesis of an E3 ligase Activity-Based Probe and its Application for the Discovery of a New Class of E3 Ligase
… yet there are currently no effective tools for profiling their activity. Herein, a new class of E3 activity-based probe (ABP) is presented which is built by re-engineering ubiquitin (Ub)-charged E2 conjugating enzymes. The utility of these probes has been demonstrated by the rapid dissection of …
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PROTEOMICS INSIGHTS INTO MOLECULAR MECHANISMS OF TRIPTOLIDE AGAINST COLORECTAL CANCER
… cytoskeleton, cell migration, cell adhesion, protein synthesis and induce alteration of transcription factors in colorectal cancer cell line HCT 116. In my first project, protein alterations of HCT 116 cells treated with triptolide were profiled at a global level, by using Isobaric Tags for …
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Altered Morphology And Composition Of Zymogen Granules In Acute Pancreatitis
… is known about the specific mechanism and the proteins and lipids that might participate in this process. Here it is reported that in acute pancreatitis, there are specific changes to both the proteome and lipidome of the ZG, contributing to altered ZG morphology and function. Using EM and AFM …
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Quantitative Approaches for Protein Differential Expression Analysis
… were developed to facilitate differential protein expression analysis and biomarker discovery via a two-step sample interrogation strategy: (a) global protein profiling and differential expression analysis by spectral counting; and, (b) biomarker candidate validation by targeted screening, …
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The Use of Proteomic Technologies to Identify Serum Glycoproteins for the Early Detection of Liver and Prostate Cancers
… of proteomic technologies to identify serum glycoproteins is an emerging technique to identify new biomarkers indicative of disease severity. Many of these newly evolving protein-profiling methodologies have evolved from previous global protein expression profiling studies such as those involving …
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Ligand-Driven Development of Pd-Catalyzed Nondirected C–H Activation of Arenes
… (CuAAC) derivatization and activity-based protein profiling (ABPP) studies. Building on the discoveries of the first study, the second project investigates more sustainable reaction conditions for dual-ligand enabled C–H olefination by reducing reliance on silver salts and …
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Mitybos įtakos aerobinėmis sąlygomis kultivuojamų Mus musculus žarnyno bakteriofagų įvairovei tyrimas /
… Bioinformatic analysis of genomic sequences, protein profiling of virions, and transmission electron microscopy (TEM) revealed the isolation of two genetically and morphologically distinct viruses: the siphovirus 50Rn infecting Enterococcus sp. strain MMBS 50 and the myovirus SuR infecting …
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Shedding Light On General Anesthesia: Uncovering The Molecular Mechanisms For Propofol And Volatile Anesthetics
… This work is dedicated to further define the protein interactions of the frequently administered volatile anesthetics sevoflurane and isoflurane, as well as the most commonly used intravenous anesthetic, propofol. A novel photoaffinity ligand for sevoflurane was validated and applied to …
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Towards Early State Disease Detection in Microdevices: Fabrication and Testing of Micro Total Analysis Systems for Bioanalytical Applications
… the characterization of the entire complement of proteins, or proteome. Micro total analysis systems (μTAS) are an emerging promising method, offering rapid, sensitive and low sample consumption separations. I have demonstrated microchip capillary electrophoresis (CE) devices made of CaF2. New …
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Proteomic profiling of Nguni cattle liver tissue using gel and Gel-Free approaches: methodology development and potential applications
… to establish a reliable set of methods for the protein profiling of Nguni cattle livers. To achieve this several alternative technologies were employed and their outcomes compared namely, two-dimensional electrophoresis, fractionation by solution phase iso-electric focusing-reversed phase …
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Chemical and biochemical studies of ubiquitin conjugation machinery
The post-translational modification of proteins is a major mechanism employed in eukaryotic cells to expand the functional diversity of the proteome. Covalent modification of amino acid side chains confers new or altered functionality to the modified protein by creating new recognition surfaces on …
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Insights into the substrate specificities, interactions and regulatory mechanisms of bacterial glycoconjugate biosynthesis enzymes
… production in the cell. However, the protein-protein interactions that comprise these complexes are poorly understood. In Chapter 3, I explore interactions among a subfamily of monoPGTs known as large (Lg) PGTs in near-native membrane mimetic systems. In this work, I characterize LgPGT …
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